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Infection and transmission dynamics of rKSHV.219 in primary endothelial cells

Kaposi's sarcoma-associated herpesvirus (KSHV) is the aetiologic agent of Kaposi's sarcoma (KS), a tumour of endothelial cell origin. The study of KS development was aided by the generation of a recombinant GFP (latent)/RFP (lytic)-expressing KSHV (rKSHV.219) by Vieira and O’Hearn (2004)....

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Autores principales: Jeffery, Hannah C., Wheat, Rachel L., Blackbourn, David J., Nash, Gerard B., Butler, Lynn M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier/North-Holland Biomedical Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147964/
https://www.ncbi.nlm.nih.gov/pubmed/23764419
http://dx.doi.org/10.1016/j.jviromet.2013.06.001
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author Jeffery, Hannah C.
Wheat, Rachel L.
Blackbourn, David J.
Nash, Gerard B.
Butler, Lynn M.
author_facet Jeffery, Hannah C.
Wheat, Rachel L.
Blackbourn, David J.
Nash, Gerard B.
Butler, Lynn M.
author_sort Jeffery, Hannah C.
collection PubMed
description Kaposi's sarcoma-associated herpesvirus (KSHV) is the aetiologic agent of Kaposi's sarcoma (KS), a tumour of endothelial cell origin. The study of KS development was aided by the generation of a recombinant GFP (latent)/RFP (lytic)-expressing KSHV (rKSHV.219) by Vieira and O’Hearn (2004). In this study the first data characterising primary endothelial cell infection and transmission with this virus is presented. Infection was predominantly latent and the percentage of GFP-positive cells increased over time. Neither horizontal transmission of infection, nor cellular proliferation, explained this increase. Analysis of latency-associated nuclear antigen (LANA-1) expression revealed that a threshold level of infection was required for GFP expression early post infection. At later time points GFP correlated more closely with LANA-1 expression, likely due to the accumulation of GFP over time. This study provides methodological guidance for the use of rKSHV.21. In addition, it highlights potential problems associated with the use of fluorescent proteins as markers of viral infection.
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spelling pubmed-41479642014-09-01 Infection and transmission dynamics of rKSHV.219 in primary endothelial cells Jeffery, Hannah C. Wheat, Rachel L. Blackbourn, David J. Nash, Gerard B. Butler, Lynn M. J Virol Methods Article Kaposi's sarcoma-associated herpesvirus (KSHV) is the aetiologic agent of Kaposi's sarcoma (KS), a tumour of endothelial cell origin. The study of KS development was aided by the generation of a recombinant GFP (latent)/RFP (lytic)-expressing KSHV (rKSHV.219) by Vieira and O’Hearn (2004). In this study the first data characterising primary endothelial cell infection and transmission with this virus is presented. Infection was predominantly latent and the percentage of GFP-positive cells increased over time. Neither horizontal transmission of infection, nor cellular proliferation, explained this increase. Analysis of latency-associated nuclear antigen (LANA-1) expression revealed that a threshold level of infection was required for GFP expression early post infection. At later time points GFP correlated more closely with LANA-1 expression, likely due to the accumulation of GFP over time. This study provides methodological guidance for the use of rKSHV.21. In addition, it highlights potential problems associated with the use of fluorescent proteins as markers of viral infection. Elsevier/North-Holland Biomedical Press 2013-10 /pmc/articles/PMC4147964/ /pubmed/23764419 http://dx.doi.org/10.1016/j.jviromet.2013.06.001 Text en © 2013 Elsevier B.V. All rights reserved. https://creativecommons.org/licenses/by/3.0/This work is licensed under a Creative Commons Attribution 3.0 Unported License (https://creativecommons.org/licenses/by/3.0/) .
spellingShingle Article
Jeffery, Hannah C.
Wheat, Rachel L.
Blackbourn, David J.
Nash, Gerard B.
Butler, Lynn M.
Infection and transmission dynamics of rKSHV.219 in primary endothelial cells
title Infection and transmission dynamics of rKSHV.219 in primary endothelial cells
title_full Infection and transmission dynamics of rKSHV.219 in primary endothelial cells
title_fullStr Infection and transmission dynamics of rKSHV.219 in primary endothelial cells
title_full_unstemmed Infection and transmission dynamics of rKSHV.219 in primary endothelial cells
title_short Infection and transmission dynamics of rKSHV.219 in primary endothelial cells
title_sort infection and transmission dynamics of rkshv.219 in primary endothelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147964/
https://www.ncbi.nlm.nih.gov/pubmed/23764419
http://dx.doi.org/10.1016/j.jviromet.2013.06.001
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