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Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis
Gastric cancer is the second leading cause of cancer-related death worldwide. Herein, we investigated the role of transcription factor Yin Yang 1 (YY1), a multi-functional protein, in tumorigenesis of gastric cancer cells. Results showed that YY1 contributed to gastric carcinogenesis of SC-M1 cells...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148117/ https://www.ncbi.nlm.nih.gov/pubmed/24970812 |
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author | Wang, An-Ming Huang, Tzu-Ting Hsu, Kai-Wen Huang, Kuo-Hung Fang, Wen-Liang Yang, Muh-Hwa Lo, Su-Shun Chi, Chin-Wen Lin, Jing-Jer Yeh, Tien-Shun |
author_facet | Wang, An-Ming Huang, Tzu-Ting Hsu, Kai-Wen Huang, Kuo-Hung Fang, Wen-Liang Yang, Muh-Hwa Lo, Su-Shun Chi, Chin-Wen Lin, Jing-Jer Yeh, Tien-Shun |
author_sort | Wang, An-Ming |
collection | PubMed |
description | Gastric cancer is the second leading cause of cancer-related death worldwide. Herein, we investigated the role of transcription factor Yin Yang 1 (YY1), a multi-functional protein, in tumorigenesis of gastric cancer cells. Results showed that YY1 contributed to gastric carcinogenesis of SC-M1 cells including growth, viability, and abilities of colony formation, migration, invasion, and tumorsphere formation. Levels of pluripotency genes CD44, Oct4, SOX-2, and Nanog were also up-regulated by YY1 in SC-M1 cells. Additionally, the 3'-untranslated region (3'-UTR) of YY1 mRNA was the target of microRNA-34 (miR-34) family consisting of miR-34a, miR-34b, and miR-34c. Overexpression of miR-34 family suppressed carcinogenesis through down-regulation of YY1 in NUGC-3 gastric cancer cells scarcely expressing miR-34 family. Alternatively, knockdown of miR-34 family promoted tumorigenesis via up-regulation of YY1 in SC-M1 and AZ521 gastric cancer cells with higher levels of miR-34 family. The miR-34 family also affected tumorsphere ultra-structure and inhibited the xenografted tumor growth as well as lung metastasis of SC-M1 cells through YY1. Expressions of miR-34a and miR-34c in gastric cancer tissues of patients were lower than those in normal tissues. Taken together, these results suggest that miR-34 family-YY1 axis plays an important role in the control of gastric carcinogenesis. |
format | Online Article Text |
id | pubmed-4148117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-41481172014-08-29 Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis Wang, An-Ming Huang, Tzu-Ting Hsu, Kai-Wen Huang, Kuo-Hung Fang, Wen-Liang Yang, Muh-Hwa Lo, Su-Shun Chi, Chin-Wen Lin, Jing-Jer Yeh, Tien-Shun Oncotarget Research Paper Gastric cancer is the second leading cause of cancer-related death worldwide. Herein, we investigated the role of transcription factor Yin Yang 1 (YY1), a multi-functional protein, in tumorigenesis of gastric cancer cells. Results showed that YY1 contributed to gastric carcinogenesis of SC-M1 cells including growth, viability, and abilities of colony formation, migration, invasion, and tumorsphere formation. Levels of pluripotency genes CD44, Oct4, SOX-2, and Nanog were also up-regulated by YY1 in SC-M1 cells. Additionally, the 3'-untranslated region (3'-UTR) of YY1 mRNA was the target of microRNA-34 (miR-34) family consisting of miR-34a, miR-34b, and miR-34c. Overexpression of miR-34 family suppressed carcinogenesis through down-regulation of YY1 in NUGC-3 gastric cancer cells scarcely expressing miR-34 family. Alternatively, knockdown of miR-34 family promoted tumorigenesis via up-regulation of YY1 in SC-M1 and AZ521 gastric cancer cells with higher levels of miR-34 family. The miR-34 family also affected tumorsphere ultra-structure and inhibited the xenografted tumor growth as well as lung metastasis of SC-M1 cells through YY1. Expressions of miR-34a and miR-34c in gastric cancer tissues of patients were lower than those in normal tissues. Taken together, these results suggest that miR-34 family-YY1 axis plays an important role in the control of gastric carcinogenesis. Impact Journals LLC 2014-06-07 /pmc/articles/PMC4148117/ /pubmed/24970812 Text en Copyright: © 2014 Wang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Wang, An-Ming Huang, Tzu-Ting Hsu, Kai-Wen Huang, Kuo-Hung Fang, Wen-Liang Yang, Muh-Hwa Lo, Su-Shun Chi, Chin-Wen Lin, Jing-Jer Yeh, Tien-Shun Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis |
title | Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis |
title_full | Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis |
title_fullStr | Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis |
title_full_unstemmed | Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis |
title_short | Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis |
title_sort | yin yang 1 is a target of microrna-34 family and contributes to gastric carcinogenesis |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148117/ https://www.ncbi.nlm.nih.gov/pubmed/24970812 |
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