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Self-processing of a barley subtilase expressed in E. coli
The barley protease BAJ93208 belongs to the subtilase family of serine proteases. We have expressed BAJ93208 in the cytoplasm of the Escherichiacoli strain SHuffle C3030 using a rhamnose-inducible promoter. The expression construct included a (His)(6)-tag at the N-terminus and a strep-tag at the C-t...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Academic Press
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148201/ https://www.ncbi.nlm.nih.gov/pubmed/24927642 http://dx.doi.org/10.1016/j.pep.2014.05.014 |