Cargando…

Transcription factor regulation and chromosome dynamics during pseudohyphal growth

Pseudohyphal growth is a developmental pathway seen in some strains of yeast in which cells form multicellular filaments in response to environmental stresses. We used multiplexed transposon “Calling Cards” to record the genome-wide binding patterns of 28 transcription factors (TFs) in nitrogen-star...

Descripción completa

Detalles Bibliográficos
Autores principales: Mayhew, David, Mitra, Robi D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148256/
https://www.ncbi.nlm.nih.gov/pubmed/25009286
http://dx.doi.org/10.1091/mbc.E14-04-0871
_version_ 1782332587752030208
author Mayhew, David
Mitra, Robi D.
author_facet Mayhew, David
Mitra, Robi D.
author_sort Mayhew, David
collection PubMed
description Pseudohyphal growth is a developmental pathway seen in some strains of yeast in which cells form multicellular filaments in response to environmental stresses. We used multiplexed transposon “Calling Cards” to record the genome-wide binding patterns of 28 transcription factors (TFs) in nitrogen-starved yeast. We identified TF targets relevant for pseudohyphal growth, producing a detailed map of its regulatory network. Using tools from graph theory, we identified 14 TFs that lie at the center of this network, including Flo8, Mss11, and Mfg1, which bind as a complex. Surprisingly, the DNA-binding preferences for these key TFs were unknown. Using Calling Card data, we predicted the in vivo DNA-binding motif for the Flo8-Mss11-Mfg1 complex and validated it using a reporter assay. We found that this complex binds several important targets, including FLO11, at both their promoter and termination sequences. We demonstrated that this binding pattern is the result of DNA looping, which regulates the transcription of these targets and is stabilized by an interaction with the nuclear pore complex. This looping provides yeast cells with a transcriptional memory, enabling them more rapidly to execute the filamentous growth program when nitrogen starved if they had been previously exposed to this condition.
format Online
Article
Text
id pubmed-4148256
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-41482562014-11-16 Transcription factor regulation and chromosome dynamics during pseudohyphal growth Mayhew, David Mitra, Robi D. Mol Biol Cell Articles Pseudohyphal growth is a developmental pathway seen in some strains of yeast in which cells form multicellular filaments in response to environmental stresses. We used multiplexed transposon “Calling Cards” to record the genome-wide binding patterns of 28 transcription factors (TFs) in nitrogen-starved yeast. We identified TF targets relevant for pseudohyphal growth, producing a detailed map of its regulatory network. Using tools from graph theory, we identified 14 TFs that lie at the center of this network, including Flo8, Mss11, and Mfg1, which bind as a complex. Surprisingly, the DNA-binding preferences for these key TFs were unknown. Using Calling Card data, we predicted the in vivo DNA-binding motif for the Flo8-Mss11-Mfg1 complex and validated it using a reporter assay. We found that this complex binds several important targets, including FLO11, at both their promoter and termination sequences. We demonstrated that this binding pattern is the result of DNA looping, which regulates the transcription of these targets and is stabilized by an interaction with the nuclear pore complex. This looping provides yeast cells with a transcriptional memory, enabling them more rapidly to execute the filamentous growth program when nitrogen starved if they had been previously exposed to this condition. The American Society for Cell Biology 2014-09-01 /pmc/articles/PMC4148256/ /pubmed/25009286 http://dx.doi.org/10.1091/mbc.E14-04-0871 Text en © 2014 Mayhew and Mitra. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Mayhew, David
Mitra, Robi D.
Transcription factor regulation and chromosome dynamics during pseudohyphal growth
title Transcription factor regulation and chromosome dynamics during pseudohyphal growth
title_full Transcription factor regulation and chromosome dynamics during pseudohyphal growth
title_fullStr Transcription factor regulation and chromosome dynamics during pseudohyphal growth
title_full_unstemmed Transcription factor regulation and chromosome dynamics during pseudohyphal growth
title_short Transcription factor regulation and chromosome dynamics during pseudohyphal growth
title_sort transcription factor regulation and chromosome dynamics during pseudohyphal growth
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148256/
https://www.ncbi.nlm.nih.gov/pubmed/25009286
http://dx.doi.org/10.1091/mbc.E14-04-0871
work_keys_str_mv AT mayhewdavid transcriptionfactorregulationandchromosomedynamicsduringpseudohyphalgrowth
AT mitrarobid transcriptionfactorregulationandchromosomedynamicsduringpseudohyphalgrowth