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Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System

Designer nucleases such as TALENS and Cas9 have opened new opportunities to scarlessly edit the mammalian genome. Here we explored several parameters that influence Cas9-mediated scarless genome editing efficiency in murine embryonic stem cells. Optimization of transfection conditions and enriching...

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Detalles Bibliográficos
Autores principales:	Li, Kai, Wang, Gang, Andersen, Troels, Zhou, Pingzhu, Pu, William T.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148324/ https://www.ncbi.nlm.nih.gov/pubmed/25166277 http://dx.doi.org/10.1371/journal.pone.0105779

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