Cargando…
Combined effect of nerve growth factor and brain-derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms
Neural stem cells (NSCs) are important pluripotent stem cells, which have potential applications in cell replacement therapy. Brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) have been demonstrated to exert a marked impact on the proliferation and differentiation of NSCs. The e...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148384/ https://www.ncbi.nlm.nih.gov/pubmed/25051506 http://dx.doi.org/10.3892/mmr.2014.2393 |
_version_ | 1782332616473575424 |
---|---|
author | LIU, FEIFEI XUAN, AIGUO CHEN, YAN ZHANG, JUNDU XU, LIPING YAN, QIJIANG LONG, DAHONG |
author_facet | LIU, FEIFEI XUAN, AIGUO CHEN, YAN ZHANG, JUNDU XU, LIPING YAN, QIJIANG LONG, DAHONG |
author_sort | LIU, FEIFEI |
collection | PubMed |
description | Neural stem cells (NSCs) are important pluripotent stem cells, which have potential applications in cell replacement therapy. Brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) have been demonstrated to exert a marked impact on the proliferation and differentiation of NSCs. The effects of NGF, BDNF, and BDNF combined with NGF on NSC neuronal differentiation and the possible mechanisms for these effects were investigated in this study. An adherent monolayer culture was employed to obtain highly homogeneous NSCs. The cells were divided into four groups: Control, NGF, BDNF and combination (BDNF + NGF) groups. Neuron differentiation was examined using immunocytochemistry and phospho-extracellular signal-regulated kinase (p-ERK) levels were analyzed using western blotting. Reverse transcription polymerase chain reaction was used to measure the mRNA expression levels of the HES1, HES5, MASH1, NGN1 and NeuroD transcription factors at different time intervals following neurotrophin-induced differentiation. NGF and BDNF were observed to induce NSC neuronal differentiation, and β-tubulin III-positive cells and p-ERK expression levels were highest in the NGF + BDNF combination group at all time points. The proportion of β-tubulin III-positive neurons in each group was associated with the expression levels of MASH1, NGN1 and NeuroD in the group. In conclusion, BDNF combined with NGF significantly improved NSC neuronal differentiation, which may provide support for the practical application of NSCs in neurodegenerative diseases. |
format | Online Article Text |
id | pubmed-4148384 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-41483842014-08-29 Combined effect of nerve growth factor and brain-derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms LIU, FEIFEI XUAN, AIGUO CHEN, YAN ZHANG, JUNDU XU, LIPING YAN, QIJIANG LONG, DAHONG Mol Med Rep Articles Neural stem cells (NSCs) are important pluripotent stem cells, which have potential applications in cell replacement therapy. Brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) have been demonstrated to exert a marked impact on the proliferation and differentiation of NSCs. The effects of NGF, BDNF, and BDNF combined with NGF on NSC neuronal differentiation and the possible mechanisms for these effects were investigated in this study. An adherent monolayer culture was employed to obtain highly homogeneous NSCs. The cells were divided into four groups: Control, NGF, BDNF and combination (BDNF + NGF) groups. Neuron differentiation was examined using immunocytochemistry and phospho-extracellular signal-regulated kinase (p-ERK) levels were analyzed using western blotting. Reverse transcription polymerase chain reaction was used to measure the mRNA expression levels of the HES1, HES5, MASH1, NGN1 and NeuroD transcription factors at different time intervals following neurotrophin-induced differentiation. NGF and BDNF were observed to induce NSC neuronal differentiation, and β-tubulin III-positive cells and p-ERK expression levels were highest in the NGF + BDNF combination group at all time points. The proportion of β-tubulin III-positive neurons in each group was associated with the expression levels of MASH1, NGN1 and NeuroD in the group. In conclusion, BDNF combined with NGF significantly improved NSC neuronal differentiation, which may provide support for the practical application of NSCs in neurodegenerative diseases. D.A. Spandidos 2014-10 2014-07-18 /pmc/articles/PMC4148384/ /pubmed/25051506 http://dx.doi.org/10.3892/mmr.2014.2393 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles LIU, FEIFEI XUAN, AIGUO CHEN, YAN ZHANG, JUNDU XU, LIPING YAN, QIJIANG LONG, DAHONG Combined effect of nerve growth factor and brain-derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms |
title | Combined effect of nerve growth factor and brain-derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms |
title_full | Combined effect of nerve growth factor and brain-derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms |
title_fullStr | Combined effect of nerve growth factor and brain-derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms |
title_full_unstemmed | Combined effect of nerve growth factor and brain-derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms |
title_short | Combined effect of nerve growth factor and brain-derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms |
title_sort | combined effect of nerve growth factor and brain-derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148384/ https://www.ncbi.nlm.nih.gov/pubmed/25051506 http://dx.doi.org/10.3892/mmr.2014.2393 |
work_keys_str_mv | AT liufeifei combinedeffectofnervegrowthfactorandbrainderivedneurotrophicfactoronneuronaldifferentiationofneuralstemcellsandthepotentialmolecularmechanisms AT xuanaiguo combinedeffectofnervegrowthfactorandbrainderivedneurotrophicfactoronneuronaldifferentiationofneuralstemcellsandthepotentialmolecularmechanisms AT chenyan combinedeffectofnervegrowthfactorandbrainderivedneurotrophicfactoronneuronaldifferentiationofneuralstemcellsandthepotentialmolecularmechanisms AT zhangjundu combinedeffectofnervegrowthfactorandbrainderivedneurotrophicfactoronneuronaldifferentiationofneuralstemcellsandthepotentialmolecularmechanisms AT xuliping combinedeffectofnervegrowthfactorandbrainderivedneurotrophicfactoronneuronaldifferentiationofneuralstemcellsandthepotentialmolecularmechanisms AT yanqijiang combinedeffectofnervegrowthfactorandbrainderivedneurotrophicfactoronneuronaldifferentiationofneuralstemcellsandthepotentialmolecularmechanisms AT longdahong combinedeffectofnervegrowthfactorandbrainderivedneurotrophicfactoronneuronaldifferentiationofneuralstemcellsandthepotentialmolecularmechanisms |