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Paracrine effect of the bone morphogeneticprotein-2 at the experimental site on healing of the adjacent control site: a study in the rabbit calvarial defect model

PURPOSE: The aim of this study was to assess the possible paracrine effect of bone morphogeneticprotein-2 (BMP-2) at the experimental site on the adjacent control site for validating a rabbit calvarial defect model as a means of verifying the effect of BMP-2. METHODS: Sixteen rabbits were divided in...

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Autores principales: Lee, Jin-Wook, Lim, Hyun-Chang, Lee, Eun-Ung, Park, Jin-Young, Lee, Jung-Seok, Lee, Dong-Woon, Jung, Ui-Won, Choi, Seong-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Academy of Periodontology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148630/
https://www.ncbi.nlm.nih.gov/pubmed/25177519
http://dx.doi.org/10.5051/jpis.2014.44.4.178
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author Lee, Jin-Wook
Lim, Hyun-Chang
Lee, Eun-Ung
Park, Jin-Young
Lee, Jung-Seok
Lee, Dong-Woon
Jung, Ui-Won
Choi, Seong-Ho
author_facet Lee, Jin-Wook
Lim, Hyun-Chang
Lee, Eun-Ung
Park, Jin-Young
Lee, Jung-Seok
Lee, Dong-Woon
Jung, Ui-Won
Choi, Seong-Ho
author_sort Lee, Jin-Wook
collection PubMed
description PURPOSE: The aim of this study was to assess the possible paracrine effect of bone morphogeneticprotein-2 (BMP-2) at the experimental site on the adjacent control site for validating a rabbit calvarial defect model as a means of verifying the effect of BMP-2. METHODS: Sixteen rabbits were divided into two groups (n=8 in each) according to whether or not BMP-2 would be used. Two circular defects (8 mm in diameter) were created side by side, 2 mm apart, in the calvarium of all of the rabbits. In each animal, one of the defects was grafted with either BMP-2-loaded carrier or carrier material alone. The control defects adjacent to these grafted defects, designated CB (the nongrafted defect adjacent BMP-2-loaded carrier-grafted defect) and CC (the nongrafted defect adjacent to carrier only-grafted defect), respectively, were the focus of this study, and were filled only with a blood clot in all of the animals. Histologic observation and histomorphometric analysis were performed at 2 and 8 weeks (n=4 animals per point in time) after surgery. RESULTS: There was no noteworthy difference in the healing pattern, and no statistically significant differences in histomorphometric parameters such as the defect closure, new bone area, or total augmented area between the CC and CB groups. CONCLUSIONS: The results of this study suggest that rabbit calvarial defects separated by a distance of 2 mm are suitable for evaluating the effects of BMP-2 and the control defect can be regarded not to be affected by BMP-2 applied defect. GRAPHICAL ABSTRACT: [Image: see text]
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spelling pubmed-41486302014-08-29 Paracrine effect of the bone morphogeneticprotein-2 at the experimental site on healing of the adjacent control site: a study in the rabbit calvarial defect model Lee, Jin-Wook Lim, Hyun-Chang Lee, Eun-Ung Park, Jin-Young Lee, Jung-Seok Lee, Dong-Woon Jung, Ui-Won Choi, Seong-Ho J Periodontal Implant Sci Research Article PURPOSE: The aim of this study was to assess the possible paracrine effect of bone morphogeneticprotein-2 (BMP-2) at the experimental site on the adjacent control site for validating a rabbit calvarial defect model as a means of verifying the effect of BMP-2. METHODS: Sixteen rabbits were divided into two groups (n=8 in each) according to whether or not BMP-2 would be used. Two circular defects (8 mm in diameter) were created side by side, 2 mm apart, in the calvarium of all of the rabbits. In each animal, one of the defects was grafted with either BMP-2-loaded carrier or carrier material alone. The control defects adjacent to these grafted defects, designated CB (the nongrafted defect adjacent BMP-2-loaded carrier-grafted defect) and CC (the nongrafted defect adjacent to carrier only-grafted defect), respectively, were the focus of this study, and were filled only with a blood clot in all of the animals. Histologic observation and histomorphometric analysis were performed at 2 and 8 weeks (n=4 animals per point in time) after surgery. RESULTS: There was no noteworthy difference in the healing pattern, and no statistically significant differences in histomorphometric parameters such as the defect closure, new bone area, or total augmented area between the CC and CB groups. CONCLUSIONS: The results of this study suggest that rabbit calvarial defects separated by a distance of 2 mm are suitable for evaluating the effects of BMP-2 and the control defect can be regarded not to be affected by BMP-2 applied defect. GRAPHICAL ABSTRACT: [Image: see text] Korean Academy of Periodontology 2014-08 2014-08-28 /pmc/articles/PMC4148630/ /pubmed/25177519 http://dx.doi.org/10.5051/jpis.2014.44.4.178 Text en Copyright © 2014 Korean Academy of Periodontology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/).
spellingShingle Research Article
Lee, Jin-Wook
Lim, Hyun-Chang
Lee, Eun-Ung
Park, Jin-Young
Lee, Jung-Seok
Lee, Dong-Woon
Jung, Ui-Won
Choi, Seong-Ho
Paracrine effect of the bone morphogeneticprotein-2 at the experimental site on healing of the adjacent control site: a study in the rabbit calvarial defect model
title Paracrine effect of the bone morphogeneticprotein-2 at the experimental site on healing of the adjacent control site: a study in the rabbit calvarial defect model
title_full Paracrine effect of the bone morphogeneticprotein-2 at the experimental site on healing of the adjacent control site: a study in the rabbit calvarial defect model
title_fullStr Paracrine effect of the bone morphogeneticprotein-2 at the experimental site on healing of the adjacent control site: a study in the rabbit calvarial defect model
title_full_unstemmed Paracrine effect of the bone morphogeneticprotein-2 at the experimental site on healing of the adjacent control site: a study in the rabbit calvarial defect model
title_short Paracrine effect of the bone morphogeneticprotein-2 at the experimental site on healing of the adjacent control site: a study in the rabbit calvarial defect model
title_sort paracrine effect of the bone morphogeneticprotein-2 at the experimental site on healing of the adjacent control site: a study in the rabbit calvarial defect model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148630/
https://www.ncbi.nlm.nih.gov/pubmed/25177519
http://dx.doi.org/10.5051/jpis.2014.44.4.178
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