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Influence of RNA extraction methods and library selection schemes on RNA-seq data
BACKGROUND: Gene expression analysis by RNA sequencing is now widely used in a number of applications surveying the whole transcriptomes of cells and tissues. The recent introduction of ribosomal RNA depletion protocols, such as RiboZero, has extended the view of the polyadenylated transcriptome to...
Autores principales: | Sultan, Marc, Amstislavskiy, Vyacheslav, Risch, Thomas, Schuette, Moritz, Dökel, Simon, Ralser, Meryem, Balzereit, Daniela, Lehrach, Hans, Yaspo, Marie-Laure |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148917/ https://www.ncbi.nlm.nih.gov/pubmed/25113896 http://dx.doi.org/10.1186/1471-2164-15-675 |
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