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High-quality draft genome sequence of nematocidal Bacillus thuringiensis Sbt003
Bacillus thuringiensis represents one of the six species of "Bacillus cereus group" in the genus Bacillus within the family Bacillaceae. Strain Sbt003 was isolated from soil and identified as B. thuringiensis. It harbors at least seven plasmids and produces three shapes of parasporal cryst...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Michigan State University
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148969/ https://www.ncbi.nlm.nih.gov/pubmed/25197449 http://dx.doi.org/10.4056/sigs.4738557 |
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author | Liu, Yingying Ye, Weixing Zheng, Jinshui Fang, Lei Peng, Donghai Ruan, Lifang Sun, Ming |
author_facet | Liu, Yingying Ye, Weixing Zheng, Jinshui Fang, Lei Peng, Donghai Ruan, Lifang Sun, Ming |
author_sort | Liu, Yingying |
collection | PubMed |
description | Bacillus thuringiensis represents one of the six species of "Bacillus cereus group" in the genus Bacillus within the family Bacillaceae. Strain Sbt003 was isolated from soil and identified as B. thuringiensis. It harbors at least seven plasmids and produces three shapes of parasporal crystals including oval, bipyramidal and rice. SDS-PAGE analysis of spore-crystal suspension of this strain reveals six major protein bands, which implies the presence of multiple parasporal crystal genes. Bioassay of this strain reveals that it shows specific activity against nematodes and human cancer cells. In this study, we report the whole genomic shotgun sequences of Sbt003. The high-quality draft of the genome is 6,175,670 bp long (including chromosome and plasmids) with 6,372 protein-coding and 80 RNA genes. |
format | Online Article Text |
id | pubmed-4148969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Michigan State University |
record_format | MEDLINE/PubMed |
spelling | pubmed-41489692014-09-05 High-quality draft genome sequence of nematocidal Bacillus thuringiensis Sbt003 Liu, Yingying Ye, Weixing Zheng, Jinshui Fang, Lei Peng, Donghai Ruan, Lifang Sun, Ming Stand Genomic Sci Short Genome Reports Bacillus thuringiensis represents one of the six species of "Bacillus cereus group" in the genus Bacillus within the family Bacillaceae. Strain Sbt003 was isolated from soil and identified as B. thuringiensis. It harbors at least seven plasmids and produces three shapes of parasporal crystals including oval, bipyramidal and rice. SDS-PAGE analysis of spore-crystal suspension of this strain reveals six major protein bands, which implies the presence of multiple parasporal crystal genes. Bioassay of this strain reveals that it shows specific activity against nematodes and human cancer cells. In this study, we report the whole genomic shotgun sequences of Sbt003. The high-quality draft of the genome is 6,175,670 bp long (including chromosome and plasmids) with 6,372 protein-coding and 80 RNA genes. Michigan State University 2014-03-01 /pmc/articles/PMC4148969/ /pubmed/25197449 http://dx.doi.org/10.4056/sigs.4738557 Text en Copyright © retained by original authors http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Genome Reports Liu, Yingying Ye, Weixing Zheng, Jinshui Fang, Lei Peng, Donghai Ruan, Lifang Sun, Ming High-quality draft genome sequence of nematocidal Bacillus thuringiensis Sbt003 |
title | High-quality draft genome sequence of nematocidal Bacillus thuringiensis Sbt003 |
title_full | High-quality draft genome sequence of nematocidal Bacillus thuringiensis Sbt003 |
title_fullStr | High-quality draft genome sequence of nematocidal Bacillus thuringiensis Sbt003 |
title_full_unstemmed | High-quality draft genome sequence of nematocidal Bacillus thuringiensis Sbt003 |
title_short | High-quality draft genome sequence of nematocidal Bacillus thuringiensis Sbt003 |
title_sort | high-quality draft genome sequence of nematocidal bacillus thuringiensis sbt003 |
topic | Short Genome Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148969/ https://www.ncbi.nlm.nih.gov/pubmed/25197449 http://dx.doi.org/10.4056/sigs.4738557 |
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