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Triglycerides in the Human Kidney Cortex: Relationship with Body Size

Obesity is associated with increased risk for kidney disease and uric acid nephrolithiasis, but the pathophysiological mechanisms underpinning these associations are incompletely understood. Animal experiments have suggested that renal lipid accumulation and lipotoxicity may play a role, but whether...

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Autores principales: Bobulescu, Ion Alexandru, Lotan, Yair, Zhang, Jianning, Rosenthal, Tara R., Rogers, John T., Adams-Huet, Beverley, Sakhaee, Khashayar, Moe, Orson W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4149342/
https://www.ncbi.nlm.nih.gov/pubmed/25170827
http://dx.doi.org/10.1371/journal.pone.0101285
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author Bobulescu, Ion Alexandru
Lotan, Yair
Zhang, Jianning
Rosenthal, Tara R.
Rogers, John T.
Adams-Huet, Beverley
Sakhaee, Khashayar
Moe, Orson W.
author_facet Bobulescu, Ion Alexandru
Lotan, Yair
Zhang, Jianning
Rosenthal, Tara R.
Rogers, John T.
Adams-Huet, Beverley
Sakhaee, Khashayar
Moe, Orson W.
author_sort Bobulescu, Ion Alexandru
collection PubMed
description Obesity is associated with increased risk for kidney disease and uric acid nephrolithiasis, but the pathophysiological mechanisms underpinning these associations are incompletely understood. Animal experiments have suggested that renal lipid accumulation and lipotoxicity may play a role, but whether lipid accumulation occurs in humans with increasing body mass index (BMI) is unknown. The association between obesity and abnormal triglyceride accumulation in non-adipose tissues (steatosis) has been described in the liver, heart, skeletal muscle and pancreas, but not in the human kidney. We used a quantitative biochemical assay to quantify triglyceride in normal kidney cortex samples from 54 patients undergoing nephrectomy for localized renal cell carcinoma. In subsets of the study population we evaluated the localization of lipid droplets by Oil Red O staining and measured 16 common ceramide species by mass spectrometry. There was a positive correlation between kidney cortex trigyceride content and BMI (Spearman R = 0.27, P = 0.04). Lipid droplets detectable by optical microscopy had a sporadic distribution but were generally more prevalent in individuals with higher BMI, with predominant localization in proximal tubule cells and to a lesser extent in glomeruli. Total ceramide content was inversely correlated with triglycerides. We postulate that obesity is associated with abnormal triglyceride accumulation (steatosis) in the human kidney. In turn, steatosis and lipotoxicity may contribute to the pathogenesis of obesity-associated kidney disease and nephrolithiasis.
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spelling pubmed-41493422014-09-03 Triglycerides in the Human Kidney Cortex: Relationship with Body Size Bobulescu, Ion Alexandru Lotan, Yair Zhang, Jianning Rosenthal, Tara R. Rogers, John T. Adams-Huet, Beverley Sakhaee, Khashayar Moe, Orson W. PLoS One Research Article Obesity is associated with increased risk for kidney disease and uric acid nephrolithiasis, but the pathophysiological mechanisms underpinning these associations are incompletely understood. Animal experiments have suggested that renal lipid accumulation and lipotoxicity may play a role, but whether lipid accumulation occurs in humans with increasing body mass index (BMI) is unknown. The association between obesity and abnormal triglyceride accumulation in non-adipose tissues (steatosis) has been described in the liver, heart, skeletal muscle and pancreas, but not in the human kidney. We used a quantitative biochemical assay to quantify triglyceride in normal kidney cortex samples from 54 patients undergoing nephrectomy for localized renal cell carcinoma. In subsets of the study population we evaluated the localization of lipid droplets by Oil Red O staining and measured 16 common ceramide species by mass spectrometry. There was a positive correlation between kidney cortex trigyceride content and BMI (Spearman R = 0.27, P = 0.04). Lipid droplets detectable by optical microscopy had a sporadic distribution but were generally more prevalent in individuals with higher BMI, with predominant localization in proximal tubule cells and to a lesser extent in glomeruli. Total ceramide content was inversely correlated with triglycerides. We postulate that obesity is associated with abnormal triglyceride accumulation (steatosis) in the human kidney. In turn, steatosis and lipotoxicity may contribute to the pathogenesis of obesity-associated kidney disease and nephrolithiasis. Public Library of Science 2014-08-29 /pmc/articles/PMC4149342/ /pubmed/25170827 http://dx.doi.org/10.1371/journal.pone.0101285 Text en © 2014 Bobulescu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bobulescu, Ion Alexandru
Lotan, Yair
Zhang, Jianning
Rosenthal, Tara R.
Rogers, John T.
Adams-Huet, Beverley
Sakhaee, Khashayar
Moe, Orson W.
Triglycerides in the Human Kidney Cortex: Relationship with Body Size
title Triglycerides in the Human Kidney Cortex: Relationship with Body Size
title_full Triglycerides in the Human Kidney Cortex: Relationship with Body Size
title_fullStr Triglycerides in the Human Kidney Cortex: Relationship with Body Size
title_full_unstemmed Triglycerides in the Human Kidney Cortex: Relationship with Body Size
title_short Triglycerides in the Human Kidney Cortex: Relationship with Body Size
title_sort triglycerides in the human kidney cortex: relationship with body size
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4149342/
https://www.ncbi.nlm.nih.gov/pubmed/25170827
http://dx.doi.org/10.1371/journal.pone.0101285
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