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Novel Strategy of Using Methyl Esters as Slow Release Methanol Source during Lipase Expression by mut(+) Pichia pastoris X33
One of the major issues with heterologous production of proteins in Pichia pastoris X33 under AOX1 promoter is repeated methanol induction. To obviate repeated methanol induction, methyl esters were used as a slow release source of methanol in lipase expressing mut(+) recombinant. Experimental desig...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4149357/ https://www.ncbi.nlm.nih.gov/pubmed/25170843 http://dx.doi.org/10.1371/journal.pone.0104272 |