Dielectric Barrier Discharge Ionization in Characterization of Organic Compounds Separated on Thin-Layer Chromatography Plates

A new method for on-spot detection and characterization of organic compounds resolved on thin layer chromatography (TLC) plates has been proposed. This method combines TLC with dielectric barrier discharge ionization (DBDI), which produces stable low-temperature plasma. At first, the compounds were...

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Detalles Bibliográficos
Autores principales: Cegłowski, Michał, Smoluch, Marek, Babij, Michał, Gotszalk, Teodor, Silberring, Jerzy, Schroeder, Grzegorz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4149504/
https://www.ncbi.nlm.nih.gov/pubmed/25170762
http://dx.doi.org/10.1371/journal.pone.0106088
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author Cegłowski, Michał
Smoluch, Marek
Babij, Michał
Gotszalk, Teodor
Silberring, Jerzy
Schroeder, Grzegorz
author_facet Cegłowski, Michał
Smoluch, Marek
Babij, Michał
Gotszalk, Teodor
Silberring, Jerzy
Schroeder, Grzegorz
author_sort Cegłowski, Michał
collection PubMed
description A new method for on-spot detection and characterization of organic compounds resolved on thin layer chromatography (TLC) plates has been proposed. This method combines TLC with dielectric barrier discharge ionization (DBDI), which produces stable low-temperature plasma. At first, the compounds were separated on TLC plates and then their mass spectra were directly obtained with no additional sample preparation. To obtain good quality spectra the center of a particular TLC spot was heated from the bottom to increase volatility of the compound. MS/MS analyses were also performed to additionally characterize all analytes. The detection limit of proposed method was estimated to be 100 ng/spot of compound.
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spelling pubmed-41495042014-09-03 Dielectric Barrier Discharge Ionization in Characterization of Organic Compounds Separated on Thin-Layer Chromatography Plates Cegłowski, Michał Smoluch, Marek Babij, Michał Gotszalk, Teodor Silberring, Jerzy Schroeder, Grzegorz PLoS One Research Article A new method for on-spot detection and characterization of organic compounds resolved on thin layer chromatography (TLC) plates has been proposed. This method combines TLC with dielectric barrier discharge ionization (DBDI), which produces stable low-temperature plasma. At first, the compounds were separated on TLC plates and then their mass spectra were directly obtained with no additional sample preparation. To obtain good quality spectra the center of a particular TLC spot was heated from the bottom to increase volatility of the compound. MS/MS analyses were also performed to additionally characterize all analytes. The detection limit of proposed method was estimated to be 100 ng/spot of compound. Public Library of Science 2014-08-29 /pmc/articles/PMC4149504/ /pubmed/25170762 http://dx.doi.org/10.1371/journal.pone.0106088 Text en © 2014 et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cegłowski, Michał
Smoluch, Marek
Babij, Michał
Gotszalk, Teodor
Silberring, Jerzy
Schroeder, Grzegorz
Dielectric Barrier Discharge Ionization in Characterization of Organic Compounds Separated on Thin-Layer Chromatography Plates
title Dielectric Barrier Discharge Ionization in Characterization of Organic Compounds Separated on Thin-Layer Chromatography Plates
title_full Dielectric Barrier Discharge Ionization in Characterization of Organic Compounds Separated on Thin-Layer Chromatography Plates
title_fullStr Dielectric Barrier Discharge Ionization in Characterization of Organic Compounds Separated on Thin-Layer Chromatography Plates
title_full_unstemmed Dielectric Barrier Discharge Ionization in Characterization of Organic Compounds Separated on Thin-Layer Chromatography Plates
title_short Dielectric Barrier Discharge Ionization in Characterization of Organic Compounds Separated on Thin-Layer Chromatography Plates
title_sort dielectric barrier discharge ionization in characterization of organic compounds separated on thin-layer chromatography plates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4149504/
https://www.ncbi.nlm.nih.gov/pubmed/25170762
http://dx.doi.org/10.1371/journal.pone.0106088
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