Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse

To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression...

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Autores principales: Bao, Wenlei, Yin, Jianxin, Liang, Yan, Guo, Zhixin, Wang, Yanfeng, Liu, Dongjun, Wang, Xiao, Wang, Zhigang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150203/
https://www.ncbi.nlm.nih.gov/pubmed/25178380
http://dx.doi.org/10.5713/ajas.2014.14046
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author Bao, Wenlei
Yin, Jianxin
Liang, Yan
Guo, Zhixin
Wang, Yanfeng
Liu, Dongjun
Wang, Xiao
Wang, Zhigang
author_facet Bao, Wenlei
Yin, Jianxin
Liang, Yan
Guo, Zhixin
Wang, Yanfeng
Liu, Dongjun
Wang, Xiao
Wang, Zhigang
author_sort Bao, Wenlei
collection PubMed
description To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C. Recombinant goat VEGF164 (rgVEGF164) was purified and identi ed by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice.
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spelling pubmed-41502032014-09-02 Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse Bao, Wenlei Yin, Jianxin Liang, Yan Guo, Zhixin Wang, Yanfeng Liu, Dongjun Wang, Xiao Wang, Zhigang Asian-Australas J Anim Sci Article To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C. Recombinant goat VEGF164 (rgVEGF164) was purified and identi ed by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2014-09 /pmc/articles/PMC4150203/ /pubmed/25178380 http://dx.doi.org/10.5713/ajas.2014.14046 Text en Copyright © 2014 by Asian-Australasian Journal of Animal Sciences This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/ which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Bao, Wenlei
Yin, Jianxin
Liang, Yan
Guo, Zhixin
Wang, Yanfeng
Liu, Dongjun
Wang, Xiao
Wang, Zhigang
Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse
title Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse
title_full Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse
title_fullStr Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse
title_full_unstemmed Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse
title_short Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse
title_sort recombinant goat vegf164 increases hair growth by painting process on the skin of shaved mouse
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150203/
https://www.ncbi.nlm.nih.gov/pubmed/25178380
http://dx.doi.org/10.5713/ajas.2014.14046
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