Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse
To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST)
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150203/ https://www.ncbi.nlm.nih.gov/pubmed/25178380 http://dx.doi.org/10.5713/ajas.2014.14046 |
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author | Bao, Wenlei Yin, Jianxin Liang, Yan Guo, Zhixin Wang, Yanfeng Liu, Dongjun Wang, Xiao Wang, Zhigang |
author_facet | Bao, Wenlei Yin, Jianxin Liang, Yan Guo, Zhixin Wang, Yanfeng Liu, Dongjun Wang, Xiao Wang, Zhigang |
author_sort | Bao, Wenlei |
collection | PubMed |
description | To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C. Recombinant goat VEGF164 (rgVEGF164) was purified and identi ed by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice. |
format | Online Article Text |
id | pubmed-4150203 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) |
record_format | MEDLINE/PubMed |
spelling | pubmed-41502032014-09-02 Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse Bao, Wenlei Yin, Jianxin Liang, Yan Guo, Zhixin Wang, Yanfeng Liu, Dongjun Wang, Xiao Wang, Zhigang Asian-Australas J Anim Sci Article To detect goat vascular endothelial growth factor (VEGF)-mediated regrowth of hair, full-length VEGF164 cDNA was cloned from Inner Mongolia cashmere goat (Capra hircus) into the pET-his prokaryotic expression vector, and the recombinant plasmid was transferred into E. coli BL21 cells. The expression of recombinant 6×his-gVEGF164 protein was induced by 0.5 mM isopropyl thio-β-D-galactoside at 32°C. Recombinant goat VEGF164 (rgVEGF164) was purified and identi ed by western blot using monoclonal anti-his and anti-VEGF antibodies. The rgVEGF164 was smeared onto the dorsal area of a shaved mouse, and we noted that hair regrowth in this area was faster than in the control group. Thus, rgVEGF164 increases hair growth in mice. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2014-09 /pmc/articles/PMC4150203/ /pubmed/25178380 http://dx.doi.org/10.5713/ajas.2014.14046 Text en Copyright © 2014 by Asian-Australasian Journal of Animal Sciences This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/ which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Bao, Wenlei Yin, Jianxin Liang, Yan Guo, Zhixin Wang, Yanfeng Liu, Dongjun Wang, Xiao Wang, Zhigang Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse |
title | Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse |
title_full | Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse |
title_fullStr | Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse |
title_full_unstemmed | Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse |
title_short | Recombinant Goat VEGF164 Increases Hair Growth by Painting Process on the Skin of Shaved Mouse |
title_sort | recombinant goat vegf164 increases hair growth by painting process on the skin of shaved mouse |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150203/ https://www.ncbi.nlm.nih.gov/pubmed/25178380 http://dx.doi.org/10.5713/ajas.2014.14046 |
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