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Oxidative Stress and NO Signalling in the Root Apex as an Early Response to Changes in Gravity Conditions
Oxygen influx showed an asymmetry in the transition zone of the root apex when roots were placed horizontally on ground. The influx increased only in the upper side, while no changes were detected in the division and in the elongation zone. Nitric oxide (NO) was also monitored after gravistimulation...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150467/ https://www.ncbi.nlm.nih.gov/pubmed/25197662 http://dx.doi.org/10.1155/2014/834134 |
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author | Mugnai, Sergio Pandolfi, Camilla Masi, Elisa Azzarello, Elisa Monetti, Emanuela Comparini, Diego Voigt, Boris Volkmann, Dieter Mancuso, Stefano |
author_facet | Mugnai, Sergio Pandolfi, Camilla Masi, Elisa Azzarello, Elisa Monetti, Emanuela Comparini, Diego Voigt, Boris Volkmann, Dieter Mancuso, Stefano |
author_sort | Mugnai, Sergio |
collection | PubMed |
description | Oxygen influx showed an asymmetry in the transition zone of the root apex when roots were placed horizontally on ground. The influx increased only in the upper side, while no changes were detected in the division and in the elongation zone. Nitric oxide (NO) was also monitored after gravistimulation, revealing a sudden burst only in the transition zone. In order to confirm these results in real microgravity conditions, experiments have been set up by using parabolic flights and drop tower. The production of reactive oxygen species (ROS) was also monitored. Oxygen, NO, and ROS were continuously monitored during normal and hyper- and microgravity conditions in roots of maize seedlings. A distinct signal in oxygen and NO fluxes was clearly detected only in the apex zone during microgravity, with no significant changes in normal and in hypergravity conditions. The same results were obtained by ROS measurement. The detrimental effect of D'orenone, disrupting the polarised auxin transport, on the onset of the oxygen peaks during the microgravity period was also evaluated. Results indicates an active role of NO and ROS as messengers during the gravitropic response, with probable implications in the auxin redistribution. |
format | Online Article Text |
id | pubmed-4150467 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41504672014-09-07 Oxidative Stress and NO Signalling in the Root Apex as an Early Response to Changes in Gravity Conditions Mugnai, Sergio Pandolfi, Camilla Masi, Elisa Azzarello, Elisa Monetti, Emanuela Comparini, Diego Voigt, Boris Volkmann, Dieter Mancuso, Stefano Biomed Res Int Research Article Oxygen influx showed an asymmetry in the transition zone of the root apex when roots were placed horizontally on ground. The influx increased only in the upper side, while no changes were detected in the division and in the elongation zone. Nitric oxide (NO) was also monitored after gravistimulation, revealing a sudden burst only in the transition zone. In order to confirm these results in real microgravity conditions, experiments have been set up by using parabolic flights and drop tower. The production of reactive oxygen species (ROS) was also monitored. Oxygen, NO, and ROS were continuously monitored during normal and hyper- and microgravity conditions in roots of maize seedlings. A distinct signal in oxygen and NO fluxes was clearly detected only in the apex zone during microgravity, with no significant changes in normal and in hypergravity conditions. The same results were obtained by ROS measurement. The detrimental effect of D'orenone, disrupting the polarised auxin transport, on the onset of the oxygen peaks during the microgravity period was also evaluated. Results indicates an active role of NO and ROS as messengers during the gravitropic response, with probable implications in the auxin redistribution. Hindawi Publishing Corporation 2014 2014-08-17 /pmc/articles/PMC4150467/ /pubmed/25197662 http://dx.doi.org/10.1155/2014/834134 Text en Copyright © 2014 Sergio Mugnai et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Mugnai, Sergio Pandolfi, Camilla Masi, Elisa Azzarello, Elisa Monetti, Emanuela Comparini, Diego Voigt, Boris Volkmann, Dieter Mancuso, Stefano Oxidative Stress and NO Signalling in the Root Apex as an Early Response to Changes in Gravity Conditions |
title | Oxidative Stress and NO Signalling in the Root Apex as an Early Response to Changes in Gravity Conditions |
title_full | Oxidative Stress and NO Signalling in the Root Apex as an Early Response to Changes in Gravity Conditions |
title_fullStr | Oxidative Stress and NO Signalling in the Root Apex as an Early Response to Changes in Gravity Conditions |
title_full_unstemmed | Oxidative Stress and NO Signalling in the Root Apex as an Early Response to Changes in Gravity Conditions |
title_short | Oxidative Stress and NO Signalling in the Root Apex as an Early Response to Changes in Gravity Conditions |
title_sort | oxidative stress and no signalling in the root apex as an early response to changes in gravity conditions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150467/ https://www.ncbi.nlm.nih.gov/pubmed/25197662 http://dx.doi.org/10.1155/2014/834134 |
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