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A fully enzymatic method for site-directed spin labeling of long RNA

Site-directed spin labeling is emerging as an essential tool to investigate the structural and dynamical features of RNA. We propose here an enzymatic method, which allows the insertion of a paramagnetic center at a specific position in an RNA molecule. The technique is based on a segmental approach...

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Detalles Bibliográficos
Autores principales:	Lebars, Isabelle, Vileno, Bertrand, Bourbigot, Sarah, Turek, Philippe, Wolff, Philippe, Kieffer, Bruno
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150755/ https://www.ncbi.nlm.nih.gov/pubmed/24981512 http://dx.doi.org/10.1093/nar/gku553

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