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The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription
Bud27, the yeast orthologue of human URI/RMP, is a member of the prefoldin-like family of ATP-independent molecular chaperones. It has recently been shown to mediate the assembly of the three RNA polymerases in an Rpb5-dependent manner. In this work, we present evidence of Bud27 modulating RNA pol I...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150788/ https://www.ncbi.nlm.nih.gov/pubmed/25081216 http://dx.doi.org/10.1093/nar/gku685 |
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author | Mirón-García, María Carmen Garrido-Godino, Ana Isabel Martínez-Fernández, Verónica Fernández-Pevida, Antonio Cuevas-Bermúdez, Abel Martín-Expósito, Manuel Chávez, Sebastián de la Cruz, Jesús Navarro, Francisco |
author_facet | Mirón-García, María Carmen Garrido-Godino, Ana Isabel Martínez-Fernández, Verónica Fernández-Pevida, Antonio Cuevas-Bermúdez, Abel Martín-Expósito, Manuel Chávez, Sebastián de la Cruz, Jesús Navarro, Francisco |
author_sort | Mirón-García, María Carmen |
collection | PubMed |
description | Bud27, the yeast orthologue of human URI/RMP, is a member of the prefoldin-like family of ATP-independent molecular chaperones. It has recently been shown to mediate the assembly of the three RNA polymerases in an Rpb5-dependent manner. In this work, we present evidence of Bud27 modulating RNA pol II transcription elongation. We show that Bud27 associates with RNA pol II phosphorylated forms (CTD-Ser5P and CTD-Ser2P), and that its absence affects RNA pol II occupancy of transcribed genes. We also reveal that Bud27 associates in vivo with the Sth1 component of the chromatin remodeling complex RSC and mediates its association with RNA pol II. Our data suggest that Bud27, in addition of contributing to Rpb5 folding within the RNA polymerases, also participates in the correct assembly of other chromatin-associated protein complexes, such as RSC, thereby modulating their activity. |
format | Online Article Text |
id | pubmed-4150788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-41507882014-12-01 The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription Mirón-García, María Carmen Garrido-Godino, Ana Isabel Martínez-Fernández, Verónica Fernández-Pevida, Antonio Cuevas-Bermúdez, Abel Martín-Expósito, Manuel Chávez, Sebastián de la Cruz, Jesús Navarro, Francisco Nucleic Acids Res Gene regulation, Chromatin and Epigenetics Bud27, the yeast orthologue of human URI/RMP, is a member of the prefoldin-like family of ATP-independent molecular chaperones. It has recently been shown to mediate the assembly of the three RNA polymerases in an Rpb5-dependent manner. In this work, we present evidence of Bud27 modulating RNA pol II transcription elongation. We show that Bud27 associates with RNA pol II phosphorylated forms (CTD-Ser5P and CTD-Ser2P), and that its absence affects RNA pol II occupancy of transcribed genes. We also reveal that Bud27 associates in vivo with the Sth1 component of the chromatin remodeling complex RSC and mediates its association with RNA pol II. Our data suggest that Bud27, in addition of contributing to Rpb5 folding within the RNA polymerases, also participates in the correct assembly of other chromatin-associated protein complexes, such as RSC, thereby modulating their activity. Oxford University Press 2014-09-02 2014-07-31 /pmc/articles/PMC4150788/ /pubmed/25081216 http://dx.doi.org/10.1093/nar/gku685 Text en © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Gene regulation, Chromatin and Epigenetics Mirón-García, María Carmen Garrido-Godino, Ana Isabel Martínez-Fernández, Verónica Fernández-Pevida, Antonio Cuevas-Bermúdez, Abel Martín-Expósito, Manuel Chávez, Sebastián de la Cruz, Jesús Navarro, Francisco The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription |
title | The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription |
title_full | The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription |
title_fullStr | The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription |
title_full_unstemmed | The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription |
title_short | The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription |
title_sort | yeast prefoldin-like uri-orthologue bud27 associates with the rsc nucleosome remodeler and modulates transcription |
topic | Gene regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150788/ https://www.ncbi.nlm.nih.gov/pubmed/25081216 http://dx.doi.org/10.1093/nar/gku685 |
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