Cargando…
Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue
BACKGROUND: Intestinal bladder augmentation has more disadvantages. One of the most promising alternative methods is tissue engineering in combination with surgical construction. Small intestine submucosa (SIS) is commonly used materials in tissue engineer. The aim of this study is determine the his...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150863/ https://www.ncbi.nlm.nih.gov/pubmed/25148849 http://dx.doi.org/10.1186/1471-2490-14-69 |
_version_ | 1782332962521481216 |
---|---|
author | Wang, Yiming Liao, Limin |
author_facet | Wang, Yiming Liao, Limin |
author_sort | Wang, Yiming |
collection | PubMed |
description | BACKGROUND: Intestinal bladder augmentation has more disadvantages. One of the most promising alternative methods is tissue engineering in combination with surgical construction. Small intestine submucosa (SIS) is commonly used materials in tissue engineer. The aim of this study is determine the histologic and functional characteristics of SIS as bladder wall replacement in a rabbit augmentation model. METHODS: 18 New Zealand adult male rabbits, weight 2.5 ± 0.5Kg, were used in this study. The rabbits were divided into 3 groups of 6 based on the number of days post-operative (A, 4 weeks; B, 12 weeks; C, 24 weeks). All of the animals underwent urodynamic testing under anesthesia before cystoplasty with SIS patch. The cystometrograms were repeated 4, 12, and 24 weeks after surgery with the same method. SIS-regenerated bladder strips (10 × 3 × 3 mm) and normal bladder strips (10 × 3 × 3 mm) from the same bladder were obtained at 4, 12, and 24 weeks for in vitro detrusor strip study. The frequency and amplitude of the strip over 15 min was recorded. The regenerated tissue and normal tissue underwent histologic and immunocytochemical analysis. The results were quantified as optical density (OD) values. RESULTS: Histologically, the SIS-regenerated bladders of group C (24 weeks post-operation) resembled normal bladder in that all 3 layers (mucosa with submucosa, smooth muscle, and serosa) were present. In the in vitro detrusor strip study, there were no significant differences in autorhythmicity and contractility between regenerated and normal tissues in group C (p > 0.05). Immunohistochemical analysis indicated that the quantity of A-actin grew to a normal level. Urodynamic testing showed that compliance remained stable in all groups post-operatively, and the volume increased 24 weeks post-operatively. CONCLUSION: Regenerated tissue has similar histologic and functional characteristics. SIS seems to be a viable material in the reconstruction of the rabbit urinary bladder. |
format | Online Article Text |
id | pubmed-4150863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41508632014-09-03 Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue Wang, Yiming Liao, Limin BMC Urol Research Article BACKGROUND: Intestinal bladder augmentation has more disadvantages. One of the most promising alternative methods is tissue engineering in combination with surgical construction. Small intestine submucosa (SIS) is commonly used materials in tissue engineer. The aim of this study is determine the histologic and functional characteristics of SIS as bladder wall replacement in a rabbit augmentation model. METHODS: 18 New Zealand adult male rabbits, weight 2.5 ± 0.5Kg, were used in this study. The rabbits were divided into 3 groups of 6 based on the number of days post-operative (A, 4 weeks; B, 12 weeks; C, 24 weeks). All of the animals underwent urodynamic testing under anesthesia before cystoplasty with SIS patch. The cystometrograms were repeated 4, 12, and 24 weeks after surgery with the same method. SIS-regenerated bladder strips (10 × 3 × 3 mm) and normal bladder strips (10 × 3 × 3 mm) from the same bladder were obtained at 4, 12, and 24 weeks for in vitro detrusor strip study. The frequency and amplitude of the strip over 15 min was recorded. The regenerated tissue and normal tissue underwent histologic and immunocytochemical analysis. The results were quantified as optical density (OD) values. RESULTS: Histologically, the SIS-regenerated bladders of group C (24 weeks post-operation) resembled normal bladder in that all 3 layers (mucosa with submucosa, smooth muscle, and serosa) were present. In the in vitro detrusor strip study, there were no significant differences in autorhythmicity and contractility between regenerated and normal tissues in group C (p > 0.05). Immunohistochemical analysis indicated that the quantity of A-actin grew to a normal level. Urodynamic testing showed that compliance remained stable in all groups post-operatively, and the volume increased 24 weeks post-operatively. CONCLUSION: Regenerated tissue has similar histologic and functional characteristics. SIS seems to be a viable material in the reconstruction of the rabbit urinary bladder. BioMed Central 2014-08-23 /pmc/articles/PMC4150863/ /pubmed/25148849 http://dx.doi.org/10.1186/1471-2490-14-69 Text en Copyright © 2014 Wang and Liao; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Wang, Yiming Liao, Limin Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue |
title | Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue |
title_full | Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue |
title_fullStr | Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue |
title_full_unstemmed | Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue |
title_short | Histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue |
title_sort | histologic and functional outcomes of small intestine submucosa-regenerated bladder tissue |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150863/ https://www.ncbi.nlm.nih.gov/pubmed/25148849 http://dx.doi.org/10.1186/1471-2490-14-69 |
work_keys_str_mv | AT wangyiming histologicandfunctionaloutcomesofsmallintestinesubmucosaregeneratedbladdertissue AT liaolimin histologicandfunctionaloutcomesofsmallintestinesubmucosaregeneratedbladdertissue |