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MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma

BACKGROUND: The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line. METHODS: Transwell chamber...

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Autores principales: Sun, Zhao, Meng, Changting, Wang, Shihua, Zhou, Na, Guan, Mei, Bai, Chunmei, Lu, Shan, Han, Qin, Zhao, Robert Chunhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150976/
https://www.ncbi.nlm.nih.gov/pubmed/25159494
http://dx.doi.org/10.1186/1471-2407-14-616
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author Sun, Zhao
Meng, Changting
Wang, Shihua
Zhou, Na
Guan, Mei
Bai, Chunmei
Lu, Shan
Han, Qin
Zhao, Robert Chunhua
author_facet Sun, Zhao
Meng, Changting
Wang, Shihua
Zhou, Na
Guan, Mei
Bai, Chunmei
Lu, Shan
Han, Qin
Zhao, Robert Chunhua
author_sort Sun, Zhao
collection PubMed
description BACKGROUND: The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line. METHODS: Transwell chambers (8-uM pore size; Costar) were used in the in vitro migration and invison anssay. Dual luciferase reporter gene construct and Dual luciferase reporter assay to identify the target of miR-1246. CADM1 expression was evaluated by immunohistochemistric staining. The clinical manifestations, treatments and survival were collected for statistical analysis. RESULTS: Inhibition of miR-1246 effectively reduced migration and invasion of hepatocellular carcinoma cell lines. Bioinformatics and luciferase reporter assay revealed that miR-1246 specifically targeted the 3′-UTR of Cell adhesion molecule 1 and regulated its expression. Down-regulation of CADM1 enhanced migration and invasion of HCC cell lines. Furthermore, in tumor tissues obtained from liver cancer patients, the expression of miR-1246 was negatively correlated with CADM1 and the high expression of miR-1246 combined with low expression of CADM1 might serve as a risk factor for stage1 liver cancer patients. CONCLUSIONS: Our study showed that miR-1246, by down-regulation CADM1, enhances migration and invasion in HCC cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2407-14-616) contains supplementary material, which is available to authorized users.
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spelling pubmed-41509762014-09-03 MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma Sun, Zhao Meng, Changting Wang, Shihua Zhou, Na Guan, Mei Bai, Chunmei Lu, Shan Han, Qin Zhao, Robert Chunhua BMC Cancer Research Article BACKGROUND: The aberrant expression of microRNAs has been demonstrated to play a crucial role in the initiation and progression of hepatocarcinoma. miR-1246 expression in High invasive ability cell line than significantly higher than that in low invasive ability cell line. METHODS: Transwell chambers (8-uM pore size; Costar) were used in the in vitro migration and invison anssay. Dual luciferase reporter gene construct and Dual luciferase reporter assay to identify the target of miR-1246. CADM1 expression was evaluated by immunohistochemistric staining. The clinical manifestations, treatments and survival were collected for statistical analysis. RESULTS: Inhibition of miR-1246 effectively reduced migration and invasion of hepatocellular carcinoma cell lines. Bioinformatics and luciferase reporter assay revealed that miR-1246 specifically targeted the 3′-UTR of Cell adhesion molecule 1 and regulated its expression. Down-regulation of CADM1 enhanced migration and invasion of HCC cell lines. Furthermore, in tumor tissues obtained from liver cancer patients, the expression of miR-1246 was negatively correlated with CADM1 and the high expression of miR-1246 combined with low expression of CADM1 might serve as a risk factor for stage1 liver cancer patients. CONCLUSIONS: Our study showed that miR-1246, by down-regulation CADM1, enhances migration and invasion in HCC cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2407-14-616) contains supplementary material, which is available to authorized users. BioMed Central 2014-08-27 /pmc/articles/PMC4150976/ /pubmed/25159494 http://dx.doi.org/10.1186/1471-2407-14-616 Text en © Sun et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Sun, Zhao
Meng, Changting
Wang, Shihua
Zhou, Na
Guan, Mei
Bai, Chunmei
Lu, Shan
Han, Qin
Zhao, Robert Chunhua
MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma
title MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma
title_full MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma
title_fullStr MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma
title_full_unstemmed MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma
title_short MicroRNA-1246 enhances migration and invasion through CADM1 in hepatocellular carcinoma
title_sort microrna-1246 enhances migration and invasion through cadm1 in hepatocellular carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150976/
https://www.ncbi.nlm.nih.gov/pubmed/25159494
http://dx.doi.org/10.1186/1471-2407-14-616
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