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Taking Snapshots of Photosynthetic Water Oxidation Using Femtosecond X-ray Diffraction and Spectroscopy
The dioxygen we breathe is formed from water by its light-induced oxidation in photosystem II. O(2) formation takes place at a catalytic manganese cluster within milliseconds after the photosystem II reaction center is excited by three single-turnover flashes. Here we present combined X-ray emission...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4151126/ https://www.ncbi.nlm.nih.gov/pubmed/25006873 http://dx.doi.org/10.1038/ncomms5371 |
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| author | Kern, Jan Tran, Rosalie Alonso-Mori, Roberto Koroidov, Sergey Echols, Nathaniel Hattne, Johan Ibrahim, Mohamed Gul, Sheraz Laksmono, Hartawan Sierra, Raymond G. Gildea, Richard J. Han, Guangye Hellmich, Julia Lassalle-Kaiser, Benedikt Chatterjee, Ruchira Brewster, Aaron S. Stan, Claudiu A. Glöckner, Carina Lampe, Alyssa DiFiore, Dörte Milathianaki, Despina Fry, Alan R. Seibert, M. Marvin Koglin, Jason E. Gallo, Erik Uhlig, Jens Sokaras, Dimosthenis Weng, Tsu-Chien Zwart, Petrus H. Skinner, David E. Bogan, Michael J. Messerschmidt, Marc Glatzel, Pieter Williams, Garth J. Boutet, Sébastien Adams, Paul D. Zouni, Athina Messinger, Johannes Sauter, Nicholas K. Bergmann, Uwe Yano, Junko Yachandra, Vittal K. |
| author_facet | Kern, Jan Tran, Rosalie Alonso-Mori, Roberto Koroidov, Sergey Echols, Nathaniel Hattne, Johan Ibrahim, Mohamed Gul, Sheraz Laksmono, Hartawan Sierra, Raymond G. Gildea, Richard J. Han, Guangye Hellmich, Julia Lassalle-Kaiser, Benedikt Chatterjee, Ruchira Brewster, Aaron S. Stan, Claudiu A. Glöckner, Carina Lampe, Alyssa DiFiore, Dörte Milathianaki, Despina Fry, Alan R. Seibert, M. Marvin Koglin, Jason E. Gallo, Erik Uhlig, Jens Sokaras, Dimosthenis Weng, Tsu-Chien Zwart, Petrus H. Skinner, David E. Bogan, Michael J. Messerschmidt, Marc Glatzel, Pieter Williams, Garth J. Boutet, Sébastien Adams, Paul D. Zouni, Athina Messinger, Johannes Sauter, Nicholas K. Bergmann, Uwe Yano, Junko Yachandra, Vittal K. |
| author_sort | Kern, Jan |
| collection | PubMed |
| description | The dioxygen we breathe is formed from water by its light-induced oxidation in photosystem II. O(2) formation takes place at a catalytic manganese cluster within milliseconds after the photosystem II reaction center is excited by three single-turnover flashes. Here we present combined X-ray emission spectra and diffraction data of 2 flash (2F) and 3 flash (3F) photosystem II samples, and of a transient 3F′ state (250 μs after the third flash), collected under functional conditions using an X-ray free electron laser. The spectra show that the initial O-O bond formation, coupled to Mn-reduction, does not yet occur within 250 μs after the third flash. Diffraction data of all states studied exhibit an anomalous scattering signal from Mn but show no significant structural changes at the present resolution of 4.5 Å. This study represents the initial frames in a molecular movie of the structural changes during the catalytic reaction in photosystem II. |
| format | Online Article Text |
| id | pubmed-4151126 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2014 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-41511262015-01-09 Taking Snapshots of Photosynthetic Water Oxidation Using Femtosecond X-ray Diffraction and Spectroscopy Kern, Jan Tran, Rosalie Alonso-Mori, Roberto Koroidov, Sergey Echols, Nathaniel Hattne, Johan Ibrahim, Mohamed Gul, Sheraz Laksmono, Hartawan Sierra, Raymond G. Gildea, Richard J. Han, Guangye Hellmich, Julia Lassalle-Kaiser, Benedikt Chatterjee, Ruchira Brewster, Aaron S. Stan, Claudiu A. Glöckner, Carina Lampe, Alyssa DiFiore, Dörte Milathianaki, Despina Fry, Alan R. Seibert, M. Marvin Koglin, Jason E. Gallo, Erik Uhlig, Jens Sokaras, Dimosthenis Weng, Tsu-Chien Zwart, Petrus H. Skinner, David E. Bogan, Michael J. Messerschmidt, Marc Glatzel, Pieter Williams, Garth J. Boutet, Sébastien Adams, Paul D. Zouni, Athina Messinger, Johannes Sauter, Nicholas K. Bergmann, Uwe Yano, Junko Yachandra, Vittal K. Nat Commun Article The dioxygen we breathe is formed from water by its light-induced oxidation in photosystem II. O(2) formation takes place at a catalytic manganese cluster within milliseconds after the photosystem II reaction center is excited by three single-turnover flashes. Here we present combined X-ray emission spectra and diffraction data of 2 flash (2F) and 3 flash (3F) photosystem II samples, and of a transient 3F′ state (250 μs after the third flash), collected under functional conditions using an X-ray free electron laser. The spectra show that the initial O-O bond formation, coupled to Mn-reduction, does not yet occur within 250 μs after the third flash. Diffraction data of all states studied exhibit an anomalous scattering signal from Mn but show no significant structural changes at the present resolution of 4.5 Å. This study represents the initial frames in a molecular movie of the structural changes during the catalytic reaction in photosystem II. 2014-07-09 /pmc/articles/PMC4151126/ /pubmed/25006873 http://dx.doi.org/10.1038/ncomms5371 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Kern, Jan Tran, Rosalie Alonso-Mori, Roberto Koroidov, Sergey Echols, Nathaniel Hattne, Johan Ibrahim, Mohamed Gul, Sheraz Laksmono, Hartawan Sierra, Raymond G. Gildea, Richard J. Han, Guangye Hellmich, Julia Lassalle-Kaiser, Benedikt Chatterjee, Ruchira Brewster, Aaron S. Stan, Claudiu A. Glöckner, Carina Lampe, Alyssa DiFiore, Dörte Milathianaki, Despina Fry, Alan R. Seibert, M. Marvin Koglin, Jason E. Gallo, Erik Uhlig, Jens Sokaras, Dimosthenis Weng, Tsu-Chien Zwart, Petrus H. Skinner, David E. Bogan, Michael J. Messerschmidt, Marc Glatzel, Pieter Williams, Garth J. Boutet, Sébastien Adams, Paul D. Zouni, Athina Messinger, Johannes Sauter, Nicholas K. Bergmann, Uwe Yano, Junko Yachandra, Vittal K. Taking Snapshots of Photosynthetic Water Oxidation Using Femtosecond X-ray Diffraction and Spectroscopy |
| title | Taking Snapshots of Photosynthetic Water Oxidation Using Femtosecond X-ray Diffraction and Spectroscopy |
| title_full | Taking Snapshots of Photosynthetic Water Oxidation Using Femtosecond X-ray Diffraction and Spectroscopy |
| title_fullStr | Taking Snapshots of Photosynthetic Water Oxidation Using Femtosecond X-ray Diffraction and Spectroscopy |
| title_full_unstemmed | Taking Snapshots of Photosynthetic Water Oxidation Using Femtosecond X-ray Diffraction and Spectroscopy |
| title_short | Taking Snapshots of Photosynthetic Water Oxidation Using Femtosecond X-ray Diffraction and Spectroscopy |
| title_sort | taking snapshots of photosynthetic water oxidation using femtosecond x-ray diffraction and spectroscopy |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4151126/ https://www.ncbi.nlm.nih.gov/pubmed/25006873 http://dx.doi.org/10.1038/ncomms5371 |
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