Cargando…

Enamel Matrix Derivative has No Effect on the Chondrogenic Differentiation of Mesenchymal Stem Cells

Background: Treatment of large bone defects due to trauma, tumor resection, or congenital abnormalities is challenging. Bone tissue engineering using mesenchymal stem cells (MSCs) represents a promising treatment option. However, the quantity and quality of engineered bone tissue are not sufficient...

Descripción completa

Detalles Bibliográficos
Autores principales: Groeneveldt, Lisanne C., Knuth, Callie, Witte-Bouma, Janneke, O’Brien, Fergal J., Wolvius, Eppo B., Farrell, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4151337/
https://www.ncbi.nlm.nih.gov/pubmed/25229057
http://dx.doi.org/10.3389/fbioe.2014.00029
_version_ 1782333012604616704
author Groeneveldt, Lisanne C.
Knuth, Callie
Witte-Bouma, Janneke
O’Brien, Fergal J.
Wolvius, Eppo B.
Farrell, Eric
author_facet Groeneveldt, Lisanne C.
Knuth, Callie
Witte-Bouma, Janneke
O’Brien, Fergal J.
Wolvius, Eppo B.
Farrell, Eric
author_sort Groeneveldt, Lisanne C.
collection PubMed
description Background: Treatment of large bone defects due to trauma, tumor resection, or congenital abnormalities is challenging. Bone tissue engineering using mesenchymal stem cells (MSCs) represents a promising treatment option. However, the quantity and quality of engineered bone tissue are not sufficient to fill large bone defects. The aim of this study was to determine if the addition of enamel matrix derivative (EMD) improves in vitro chondrogenic priming of MSCs to ultimately improve in vivo MSC mediated endochondral bone formation. Methods: MSCs were chondrogenically differentiated in 2.0 × 10(5) cell pellets in medium supplemented with TGFβ3 in the absence or presence of 1, 10, or 100 μg/mL EMD. Samples were analyzed for gene expression of RUNX2, Col II, Col X, and Sox9. Protein and glycoaminoglycan (GAG) production were also investigated via DMB assays, histology, and immunohistochemistry. Osteogenic and adipogenic differentiation capacity were also assessed. Results: The addition of EMD did not negatively affect chondrogenic differentiation of adult human MSCs. EMD did not appear to alter GAG production or expression of chondrogenic genes. Osteogenic and adipogenic differentiation were also unaffected though a trend toward decreased adipogenic gene expression was observed. Conclusion: EMD does not affect chondrogenic differentiation of adult human MSCs. As such the use of EMD in combination with chondrogenically primed MSCs for periodontal bone tissue repair is unlikely to have negative effects on MSC differentiation.
format Online
Article
Text
id pubmed-4151337
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-41513372014-09-16 Enamel Matrix Derivative has No Effect on the Chondrogenic Differentiation of Mesenchymal Stem Cells Groeneveldt, Lisanne C. Knuth, Callie Witte-Bouma, Janneke O’Brien, Fergal J. Wolvius, Eppo B. Farrell, Eric Front Bioeng Biotechnol Bioengineering and Biotechnology Background: Treatment of large bone defects due to trauma, tumor resection, or congenital abnormalities is challenging. Bone tissue engineering using mesenchymal stem cells (MSCs) represents a promising treatment option. However, the quantity and quality of engineered bone tissue are not sufficient to fill large bone defects. The aim of this study was to determine if the addition of enamel matrix derivative (EMD) improves in vitro chondrogenic priming of MSCs to ultimately improve in vivo MSC mediated endochondral bone formation. Methods: MSCs were chondrogenically differentiated in 2.0 × 10(5) cell pellets in medium supplemented with TGFβ3 in the absence or presence of 1, 10, or 100 μg/mL EMD. Samples were analyzed for gene expression of RUNX2, Col II, Col X, and Sox9. Protein and glycoaminoglycan (GAG) production were also investigated via DMB assays, histology, and immunohistochemistry. Osteogenic and adipogenic differentiation capacity were also assessed. Results: The addition of EMD did not negatively affect chondrogenic differentiation of adult human MSCs. EMD did not appear to alter GAG production or expression of chondrogenic genes. Osteogenic and adipogenic differentiation were also unaffected though a trend toward decreased adipogenic gene expression was observed. Conclusion: EMD does not affect chondrogenic differentiation of adult human MSCs. As such the use of EMD in combination with chondrogenically primed MSCs for periodontal bone tissue repair is unlikely to have negative effects on MSC differentiation. Frontiers Media S.A. 2014-09-02 /pmc/articles/PMC4151337/ /pubmed/25229057 http://dx.doi.org/10.3389/fbioe.2014.00029 Text en Copyright © 2014 Groeneveldt, Knuth, Witte-Bouma, O’Brien, Wolvius and Farrell. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Groeneveldt, Lisanne C.
Knuth, Callie
Witte-Bouma, Janneke
O’Brien, Fergal J.
Wolvius, Eppo B.
Farrell, Eric
Enamel Matrix Derivative has No Effect on the Chondrogenic Differentiation of Mesenchymal Stem Cells
title Enamel Matrix Derivative has No Effect on the Chondrogenic Differentiation of Mesenchymal Stem Cells
title_full Enamel Matrix Derivative has No Effect on the Chondrogenic Differentiation of Mesenchymal Stem Cells
title_fullStr Enamel Matrix Derivative has No Effect on the Chondrogenic Differentiation of Mesenchymal Stem Cells
title_full_unstemmed Enamel Matrix Derivative has No Effect on the Chondrogenic Differentiation of Mesenchymal Stem Cells
title_short Enamel Matrix Derivative has No Effect on the Chondrogenic Differentiation of Mesenchymal Stem Cells
title_sort enamel matrix derivative has no effect on the chondrogenic differentiation of mesenchymal stem cells
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4151337/
https://www.ncbi.nlm.nih.gov/pubmed/25229057
http://dx.doi.org/10.3389/fbioe.2014.00029
work_keys_str_mv AT groeneveldtlisannec enamelmatrixderivativehasnoeffectonthechondrogenicdifferentiationofmesenchymalstemcells
AT knuthcallie enamelmatrixderivativehasnoeffectonthechondrogenicdifferentiationofmesenchymalstemcells
AT witteboumajanneke enamelmatrixderivativehasnoeffectonthechondrogenicdifferentiationofmesenchymalstemcells
AT obrienfergalj enamelmatrixderivativehasnoeffectonthechondrogenicdifferentiationofmesenchymalstemcells
AT wolviuseppob enamelmatrixderivativehasnoeffectonthechondrogenicdifferentiationofmesenchymalstemcells
AT farrelleric enamelmatrixderivativehasnoeffectonthechondrogenicdifferentiationofmesenchymalstemcells