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The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease

Interleukin- (IL-) 35 and IL-37 are newly discovered immune-suppressing cytokines. They have been described in inflammatory diseases such as collagen-induced arthritis and asthma. However, their expressions in inflammatory bowel disease (IBD) patients have not been yet explored. Our aim was to evalu...

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Autores principales: Li, Yanmei, Wang, Yanan, Liu, Ying, Wang, Yatian, Zuo, Xiuli, Li, Yanqing, Lu, Xuefeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4151582/
https://www.ncbi.nlm.nih.gov/pubmed/25214710
http://dx.doi.org/10.1155/2014/136329
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author Li, Yanmei
Wang, Yanan
Liu, Ying
Wang, Yatian
Zuo, Xiuli
Li, Yanqing
Lu, Xuefeng
author_facet Li, Yanmei
Wang, Yanan
Liu, Ying
Wang, Yatian
Zuo, Xiuli
Li, Yanqing
Lu, Xuefeng
author_sort Li, Yanmei
collection PubMed
description Interleukin- (IL-) 35 and IL-37 are newly discovered immune-suppressing cytokines. They have been described in inflammatory diseases such as collagen-induced arthritis and asthma. However, their expressions in inflammatory bowel disease (IBD) patients have not been yet explored. Our aim was to evaluate serum and inflamed mucosal levels in IBD patients. In 20 ulcerative colitis (UC) patients, 7 Crohn's disease (CD) patients, and 15 healthy subjects, cytokine levels in serum were determined using ELISA and mucosal expression studies were performed by immunohistochemistry, quantitative real-time PCR, and Western blot. The results showed that serums IL-35 and IL-37 levels were significantly decreased in UC and CD patients compared with healthy subjects. The cytokines levels correlated inversely with UC activity. IL-35 was expressed in infiltrating immune cells while IL-37 in intestinal epithelial cells as well as inflammatory cells. IBD patients had significantly higher Ebi3, p35 (two subunits of IL-35), and IL-37b gene expressions; IL-35 and IL-37 protein expressions were higher in IBD patients compared with controls. The study showed that serums IL-35 and IL-37 might be potentially novel biomarkers for IBD. Intestinal IL-35 and IL-37 proteins are upregulated, suggesting that regulating the expression of the two cytokines may provide a new possible target for the treatment of IBD.
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spelling pubmed-41515822014-09-11 The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease Li, Yanmei Wang, Yanan Liu, Ying Wang, Yatian Zuo, Xiuli Li, Yanqing Lu, Xuefeng Mediators Inflamm Research Article Interleukin- (IL-) 35 and IL-37 are newly discovered immune-suppressing cytokines. They have been described in inflammatory diseases such as collagen-induced arthritis and asthma. However, their expressions in inflammatory bowel disease (IBD) patients have not been yet explored. Our aim was to evaluate serum and inflamed mucosal levels in IBD patients. In 20 ulcerative colitis (UC) patients, 7 Crohn's disease (CD) patients, and 15 healthy subjects, cytokine levels in serum were determined using ELISA and mucosal expression studies were performed by immunohistochemistry, quantitative real-time PCR, and Western blot. The results showed that serums IL-35 and IL-37 levels were significantly decreased in UC and CD patients compared with healthy subjects. The cytokines levels correlated inversely with UC activity. IL-35 was expressed in infiltrating immune cells while IL-37 in intestinal epithelial cells as well as inflammatory cells. IBD patients had significantly higher Ebi3, p35 (two subunits of IL-35), and IL-37b gene expressions; IL-35 and IL-37 protein expressions were higher in IBD patients compared with controls. The study showed that serums IL-35 and IL-37 might be potentially novel biomarkers for IBD. Intestinal IL-35 and IL-37 proteins are upregulated, suggesting that regulating the expression of the two cytokines may provide a new possible target for the treatment of IBD. Hindawi Publishing Corporation 2014 2014-08-18 /pmc/articles/PMC4151582/ /pubmed/25214710 http://dx.doi.org/10.1155/2014/136329 Text en Copyright © 2014 Yanmei Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Yanmei
Wang, Yanan
Liu, Ying
Wang, Yatian
Zuo, Xiuli
Li, Yanqing
Lu, Xuefeng
The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease
title The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease
title_full The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease
title_fullStr The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease
title_full_unstemmed The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease
title_short The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease
title_sort possible role of the novel cytokines il-35 and il-37 in inflammatory bowel disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4151582/
https://www.ncbi.nlm.nih.gov/pubmed/25214710
http://dx.doi.org/10.1155/2014/136329
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