Cargando…
The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease
Interleukin- (IL-) 35 and IL-37 are newly discovered immune-suppressing cytokines. They have been described in inflammatory diseases such as collagen-induced arthritis and asthma. However, their expressions in inflammatory bowel disease (IBD) patients have not been yet explored. Our aim was to evalu...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4151582/ https://www.ncbi.nlm.nih.gov/pubmed/25214710 http://dx.doi.org/10.1155/2014/136329 |
_version_ | 1782333031323795456 |
---|---|
author | Li, Yanmei Wang, Yanan Liu, Ying Wang, Yatian Zuo, Xiuli Li, Yanqing Lu, Xuefeng |
author_facet | Li, Yanmei Wang, Yanan Liu, Ying Wang, Yatian Zuo, Xiuli Li, Yanqing Lu, Xuefeng |
author_sort | Li, Yanmei |
collection | PubMed |
description | Interleukin- (IL-) 35 and IL-37 are newly discovered immune-suppressing cytokines. They have been described in inflammatory diseases such as collagen-induced arthritis and asthma. However, their expressions in inflammatory bowel disease (IBD) patients have not been yet explored. Our aim was to evaluate serum and inflamed mucosal levels in IBD patients. In 20 ulcerative colitis (UC) patients, 7 Crohn's disease (CD) patients, and 15 healthy subjects, cytokine levels in serum were determined using ELISA and mucosal expression studies were performed by immunohistochemistry, quantitative real-time PCR, and Western blot. The results showed that serums IL-35 and IL-37 levels were significantly decreased in UC and CD patients compared with healthy subjects. The cytokines levels correlated inversely with UC activity. IL-35 was expressed in infiltrating immune cells while IL-37 in intestinal epithelial cells as well as inflammatory cells. IBD patients had significantly higher Ebi3, p35 (two subunits of IL-35), and IL-37b gene expressions; IL-35 and IL-37 protein expressions were higher in IBD patients compared with controls. The study showed that serums IL-35 and IL-37 might be potentially novel biomarkers for IBD. Intestinal IL-35 and IL-37 proteins are upregulated, suggesting that regulating the expression of the two cytokines may provide a new possible target for the treatment of IBD. |
format | Online Article Text |
id | pubmed-4151582 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41515822014-09-11 The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease Li, Yanmei Wang, Yanan Liu, Ying Wang, Yatian Zuo, Xiuli Li, Yanqing Lu, Xuefeng Mediators Inflamm Research Article Interleukin- (IL-) 35 and IL-37 are newly discovered immune-suppressing cytokines. They have been described in inflammatory diseases such as collagen-induced arthritis and asthma. However, their expressions in inflammatory bowel disease (IBD) patients have not been yet explored. Our aim was to evaluate serum and inflamed mucosal levels in IBD patients. In 20 ulcerative colitis (UC) patients, 7 Crohn's disease (CD) patients, and 15 healthy subjects, cytokine levels in serum were determined using ELISA and mucosal expression studies were performed by immunohistochemistry, quantitative real-time PCR, and Western blot. The results showed that serums IL-35 and IL-37 levels were significantly decreased in UC and CD patients compared with healthy subjects. The cytokines levels correlated inversely with UC activity. IL-35 was expressed in infiltrating immune cells while IL-37 in intestinal epithelial cells as well as inflammatory cells. IBD patients had significantly higher Ebi3, p35 (two subunits of IL-35), and IL-37b gene expressions; IL-35 and IL-37 protein expressions were higher in IBD patients compared with controls. The study showed that serums IL-35 and IL-37 might be potentially novel biomarkers for IBD. Intestinal IL-35 and IL-37 proteins are upregulated, suggesting that regulating the expression of the two cytokines may provide a new possible target for the treatment of IBD. Hindawi Publishing Corporation 2014 2014-08-18 /pmc/articles/PMC4151582/ /pubmed/25214710 http://dx.doi.org/10.1155/2014/136329 Text en Copyright © 2014 Yanmei Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Li, Yanmei Wang, Yanan Liu, Ying Wang, Yatian Zuo, Xiuli Li, Yanqing Lu, Xuefeng The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease |
title | The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease |
title_full | The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease |
title_fullStr | The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease |
title_full_unstemmed | The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease |
title_short | The Possible Role of the Novel Cytokines IL-35 and IL-37 in Inflammatory Bowel Disease |
title_sort | possible role of the novel cytokines il-35 and il-37 in inflammatory bowel disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4151582/ https://www.ncbi.nlm.nih.gov/pubmed/25214710 http://dx.doi.org/10.1155/2014/136329 |
work_keys_str_mv | AT liyanmei thepossibleroleofthenovelcytokinesil35andil37ininflammatoryboweldisease AT wangyanan thepossibleroleofthenovelcytokinesil35andil37ininflammatoryboweldisease AT liuying thepossibleroleofthenovelcytokinesil35andil37ininflammatoryboweldisease AT wangyatian thepossibleroleofthenovelcytokinesil35andil37ininflammatoryboweldisease AT zuoxiuli thepossibleroleofthenovelcytokinesil35andil37ininflammatoryboweldisease AT liyanqing thepossibleroleofthenovelcytokinesil35andil37ininflammatoryboweldisease AT luxuefeng thepossibleroleofthenovelcytokinesil35andil37ininflammatoryboweldisease AT liyanmei possibleroleofthenovelcytokinesil35andil37ininflammatoryboweldisease AT wangyanan possibleroleofthenovelcytokinesil35andil37ininflammatoryboweldisease AT liuying possibleroleofthenovelcytokinesil35andil37ininflammatoryboweldisease AT wangyatian possibleroleofthenovelcytokinesil35andil37ininflammatoryboweldisease AT zuoxiuli possibleroleofthenovelcytokinesil35andil37ininflammatoryboweldisease AT liyanqing possibleroleofthenovelcytokinesil35andil37ininflammatoryboweldisease AT luxuefeng possibleroleofthenovelcytokinesil35andil37ininflammatoryboweldisease |