Cargando…

Polyethylenimine Carbon Nanotube Fiber Electrodes for Enhanced Detection of Neurotransmitters

[Image: see text] Carbon nanotube (CNT)-based microelectrodes have been investigated as alternatives to carbon-fiber microelectrodes for the detection of neurotransmitters because they are sensitive, exhibit fast electron transfer kinetics, and are more resistant to surface fouling. Wet spinning CNT...

Descripción completa

Detalles Bibliográficos
Autores principales: Zestos, Alexander G., Jacobs, Christopher B., Trikantzopoulos, Elefterios, Ross, Ashley E., Venton, B. Jill
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4151793/
https://www.ncbi.nlm.nih.gov/pubmed/25117550
http://dx.doi.org/10.1021/ac5003273
_version_ 1782333057667170304
author Zestos, Alexander G.
Jacobs, Christopher B.
Trikantzopoulos, Elefterios
Ross, Ashley E.
Venton, B. Jill
author_facet Zestos, Alexander G.
Jacobs, Christopher B.
Trikantzopoulos, Elefterios
Ross, Ashley E.
Venton, B. Jill
author_sort Zestos, Alexander G.
collection PubMed
description [Image: see text] Carbon nanotube (CNT)-based microelectrodes have been investigated as alternatives to carbon-fiber microelectrodes for the detection of neurotransmitters because they are sensitive, exhibit fast electron transfer kinetics, and are more resistant to surface fouling. Wet spinning CNTs into fibers using a coagulating polymer produces a thin, uniform fiber that can be fabricated into an electrode. CNT fibers formed in poly(vinyl alcohol) (PVA) have been used as microelectrodes to detect dopamine, serotonin, and hydrogen peroxide. In this study, we characterize microelectrodes with CNT fibers made in polyethylenimine (PEI), which have much higher conductivity than PVA-CNT fibers. PEI-CNT fibers have lower overpotentials and higher sensitivities than PVA-CNT fiber microelectrodes, with a limit of detection of 5 nM for dopamine. The currents for dopamine were adsorption controlled at PEI-CNT fiber microelectrodes, independent of scan repetition frequency, and stable for over 10 h. PEI-CNT fiber microelectrodes were resistant to surface fouling by serotonin and the metabolite interferant 5-hydroxyindoleacetic acid (5-HIAA). No change in sensitivity was observed for detection of serotonin after 30 flow injection experiments or after 2 h in 5-HIAA for PEI-CNT electrodes. The antifouling properties were maintained in brain slices when serotonin was exogenously applied multiple times or after bathing the slice in 5-HIAA. Thus, PEI-CNT fiber electrodes could be useful for the in vivo monitoring of neurochemicals.
format Online
Article
Text
id pubmed-4151793
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-41517932015-08-13 Polyethylenimine Carbon Nanotube Fiber Electrodes for Enhanced Detection of Neurotransmitters Zestos, Alexander G. Jacobs, Christopher B. Trikantzopoulos, Elefterios Ross, Ashley E. Venton, B. Jill Anal Chem [Image: see text] Carbon nanotube (CNT)-based microelectrodes have been investigated as alternatives to carbon-fiber microelectrodes for the detection of neurotransmitters because they are sensitive, exhibit fast electron transfer kinetics, and are more resistant to surface fouling. Wet spinning CNTs into fibers using a coagulating polymer produces a thin, uniform fiber that can be fabricated into an electrode. CNT fibers formed in poly(vinyl alcohol) (PVA) have been used as microelectrodes to detect dopamine, serotonin, and hydrogen peroxide. In this study, we characterize microelectrodes with CNT fibers made in polyethylenimine (PEI), which have much higher conductivity than PVA-CNT fibers. PEI-CNT fibers have lower overpotentials and higher sensitivities than PVA-CNT fiber microelectrodes, with a limit of detection of 5 nM for dopamine. The currents for dopamine were adsorption controlled at PEI-CNT fiber microelectrodes, independent of scan repetition frequency, and stable for over 10 h. PEI-CNT fiber microelectrodes were resistant to surface fouling by serotonin and the metabolite interferant 5-hydroxyindoleacetic acid (5-HIAA). No change in sensitivity was observed for detection of serotonin after 30 flow injection experiments or after 2 h in 5-HIAA for PEI-CNT electrodes. The antifouling properties were maintained in brain slices when serotonin was exogenously applied multiple times or after bathing the slice in 5-HIAA. Thus, PEI-CNT fiber electrodes could be useful for the in vivo monitoring of neurochemicals. American Chemical Society 2014-08-13 2014-09-02 /pmc/articles/PMC4151793/ /pubmed/25117550 http://dx.doi.org/10.1021/ac5003273 Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Zestos, Alexander G.
Jacobs, Christopher B.
Trikantzopoulos, Elefterios
Ross, Ashley E.
Venton, B. Jill
Polyethylenimine Carbon Nanotube Fiber Electrodes for Enhanced Detection of Neurotransmitters
title Polyethylenimine Carbon Nanotube Fiber Electrodes for Enhanced Detection of Neurotransmitters
title_full Polyethylenimine Carbon Nanotube Fiber Electrodes for Enhanced Detection of Neurotransmitters
title_fullStr Polyethylenimine Carbon Nanotube Fiber Electrodes for Enhanced Detection of Neurotransmitters
title_full_unstemmed Polyethylenimine Carbon Nanotube Fiber Electrodes for Enhanced Detection of Neurotransmitters
title_short Polyethylenimine Carbon Nanotube Fiber Electrodes for Enhanced Detection of Neurotransmitters
title_sort polyethylenimine carbon nanotube fiber electrodes for enhanced detection of neurotransmitters
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4151793/
https://www.ncbi.nlm.nih.gov/pubmed/25117550
http://dx.doi.org/10.1021/ac5003273
work_keys_str_mv AT zestosalexanderg polyethyleniminecarbonnanotubefiberelectrodesforenhanceddetectionofneurotransmitters
AT jacobschristopherb polyethyleniminecarbonnanotubefiberelectrodesforenhanceddetectionofneurotransmitters
AT trikantzopouloselefterios polyethyleniminecarbonnanotubefiberelectrodesforenhanceddetectionofneurotransmitters
AT rossashleye polyethyleniminecarbonnanotubefiberelectrodesforenhanceddetectionofneurotransmitters
AT ventonbjill polyethyleniminecarbonnanotubefiberelectrodesforenhanceddetectionofneurotransmitters