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Characterization and Plasmid Elimination of NDM-1-Producing Acinetobacter calcoaceticus from China
The presence of multidrug-resistant bacterial pathogens in the environment poses a serious threat to public health. The opportunistic Acinetobacter spp. are among the most prevalent causes of nosocomial infections. Here, we performed complete genome sequencing of the Acinetobacter calcoaceticus stra...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4152304/ https://www.ncbi.nlm.nih.gov/pubmed/25181293 http://dx.doi.org/10.1371/journal.pone.0106555 |
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author | Sun, Yang Liu, Qi Chen, Shuo Song, Yang Liu, Jun Guo, Xuejun Zhu, Lingwei Ji, Xue Xu, Lizhi Zhou, Wei Qian, Jun Feng, Shuzhang |
author_facet | Sun, Yang Liu, Qi Chen, Shuo Song, Yang Liu, Jun Guo, Xuejun Zhu, Lingwei Ji, Xue Xu, Lizhi Zhou, Wei Qian, Jun Feng, Shuzhang |
author_sort | Sun, Yang |
collection | PubMed |
description | The presence of multidrug-resistant bacterial pathogens in the environment poses a serious threat to public health. The opportunistic Acinetobacter spp. are among the most prevalent causes of nosocomial infections. Here, we performed complete genome sequencing of the Acinetobacter calcoaceticus strain XM1570, which was originally cultivated from the sputum of a patient diagnosed with pneumonia in Xiamen in 2010. We identified carbapenem resistance associated gene bla (NDM-1) located on a 47.3-kb plasmid. Three methods – natural reproduction, sodium dodecyl sulfate treatment and nalidixic acid treatment – were used to eliminate the bla (NDM-1)-encoding plasmid, which achieved elimination rates of 3.32% (10/301), 83.78% (278/332), and 84.17% (298/354), respectively. Plasmid elimination dramatically increased antibiotic sensitivity, reducing the minimum bacteriostatic concentration of meropenem from 256 µg/ml in the clinical strain to 0.125 µg/ml in the plasmid-eliminated strain. Conjugation transfer assays showed that the bla (NDM-1)-containing plasmid could be transferred into Escherichia coli DH5α:pBR322 in vitro as well as in vivo in mice. The bla (NDM-1) genetic environment was in accordance with that of other bla (NDM-1) genes identified from India, Japan, and Hong-Kong. The multilocus sequence type of the isolate was identified as ST-70. Two novel genes encoding intrinsic OXA and ADC were identified and named as OXA-417 and ADC-72. The finding of bla (NDM-1) in species like A. calcoaceticus demonstrates the wide spread of this gene in gram-negative bacteria which is possible by conjugative plasmid transfer. The results of this study may help in the development of a treatment strategy for controlling NDM-1 bacterial infection and transmission. |
format | Online Article Text |
id | pubmed-4152304 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41523042014-09-05 Characterization and Plasmid Elimination of NDM-1-Producing Acinetobacter calcoaceticus from China Sun, Yang Liu, Qi Chen, Shuo Song, Yang Liu, Jun Guo, Xuejun Zhu, Lingwei Ji, Xue Xu, Lizhi Zhou, Wei Qian, Jun Feng, Shuzhang PLoS One Research Article The presence of multidrug-resistant bacterial pathogens in the environment poses a serious threat to public health. The opportunistic Acinetobacter spp. are among the most prevalent causes of nosocomial infections. Here, we performed complete genome sequencing of the Acinetobacter calcoaceticus strain XM1570, which was originally cultivated from the sputum of a patient diagnosed with pneumonia in Xiamen in 2010. We identified carbapenem resistance associated gene bla (NDM-1) located on a 47.3-kb plasmid. Three methods – natural reproduction, sodium dodecyl sulfate treatment and nalidixic acid treatment – were used to eliminate the bla (NDM-1)-encoding plasmid, which achieved elimination rates of 3.32% (10/301), 83.78% (278/332), and 84.17% (298/354), respectively. Plasmid elimination dramatically increased antibiotic sensitivity, reducing the minimum bacteriostatic concentration of meropenem from 256 µg/ml in the clinical strain to 0.125 µg/ml in the plasmid-eliminated strain. Conjugation transfer assays showed that the bla (NDM-1)-containing plasmid could be transferred into Escherichia coli DH5α:pBR322 in vitro as well as in vivo in mice. The bla (NDM-1) genetic environment was in accordance with that of other bla (NDM-1) genes identified from India, Japan, and Hong-Kong. The multilocus sequence type of the isolate was identified as ST-70. Two novel genes encoding intrinsic OXA and ADC were identified and named as OXA-417 and ADC-72. The finding of bla (NDM-1) in species like A. calcoaceticus demonstrates the wide spread of this gene in gram-negative bacteria which is possible by conjugative plasmid transfer. The results of this study may help in the development of a treatment strategy for controlling NDM-1 bacterial infection and transmission. Public Library of Science 2014-09-02 /pmc/articles/PMC4152304/ /pubmed/25181293 http://dx.doi.org/10.1371/journal.pone.0106555 Text en © 2014 Sun et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Sun, Yang Liu, Qi Chen, Shuo Song, Yang Liu, Jun Guo, Xuejun Zhu, Lingwei Ji, Xue Xu, Lizhi Zhou, Wei Qian, Jun Feng, Shuzhang Characterization and Plasmid Elimination of NDM-1-Producing Acinetobacter calcoaceticus from China |
title | Characterization and Plasmid Elimination of NDM-1-Producing Acinetobacter calcoaceticus from China |
title_full | Characterization and Plasmid Elimination of NDM-1-Producing Acinetobacter calcoaceticus from China |
title_fullStr | Characterization and Plasmid Elimination of NDM-1-Producing Acinetobacter calcoaceticus from China |
title_full_unstemmed | Characterization and Plasmid Elimination of NDM-1-Producing Acinetobacter calcoaceticus from China |
title_short | Characterization and Plasmid Elimination of NDM-1-Producing Acinetobacter calcoaceticus from China |
title_sort | characterization and plasmid elimination of ndm-1-producing acinetobacter calcoaceticus from china |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4152304/ https://www.ncbi.nlm.nih.gov/pubmed/25181293 http://dx.doi.org/10.1371/journal.pone.0106555 |
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