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Expression of THOP1 and Its Relationship to Prognosis in Non-Small Cell Lung Cancer
BACKGROUND: The study was designed to detect the expression level of thimet oligopeptidase (THOP1) protein in non-small cell lung cancer (NSCLC) and investigate its correlation with clinicopathologic features and prognosis. METHODS: Immunohistochemical staining was used to determine the expression o...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4152321/ https://www.ncbi.nlm.nih.gov/pubmed/25180910 http://dx.doi.org/10.1371/journal.pone.0106665 |
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author | Qi, Lei Li, Shu-hai Si, Li-bo Lu, Ming Tian, Hui |
author_facet | Qi, Lei Li, Shu-hai Si, Li-bo Lu, Ming Tian, Hui |
author_sort | Qi, Lei |
collection | PubMed |
description | BACKGROUND: The study was designed to detect the expression level of thimet oligopeptidase (THOP1) protein in non-small cell lung cancer (NSCLC) and investigate its correlation with clinicopathologic features and prognosis. METHODS: Immunohistochemical staining was used to determine the expression of THOP1 protein in 120 NSCLC specimens and 53 distant normal lung tissues. Quantitative real-time PCR and western blotting were employed to measure the expression of THOP1 in 16 pairs of primary NSCLC and corresponding normal tissues. RESULTS: Analysis of immunohistochemical staining suggested low THOP1 expression was found in 71 (59.2%) of the 120 NSCLC specimens and significantly correlated with positive lymph node metastasis (P = 0.048). However, low THOP1 expression was found in 22 (41.5%) of the 53 normal lung tissues. Chi-square test suggested that the expression of THOP1 was significantly higher in the normal lung tissues than that in the NSCLC specimens (P = 0.032). Real-Time PCR and western blotting showed that NSCLC specimens had decreased THOP1 mRNA and protein expression compared to corresponding normal tissues. Univariate analysis demonstrated that low THOP1 expression significantly predicted decreased 5-year disease-free survival (P = 0.038) and overall survival (P = 0.017). In addition, positive lymph node metastasis (P = 0.025) and advanced TNM stage (P = 0.009) significantly predicted decreased 5-year overall survival. However, multivariate Cox regression analysis showed that only low THOP1 expression retained its significance as an independent prognostic factor for unfavorable 5-year disease-free survival (P = 0.046) and overall survival (P = 0.021). CONCLUSIONS: THOP1 may have clinical potentials to be employed as a promising biomarker to identify individuals with better prognosis and a novel antitumor agent for therapy of patients with NSCLC. |
format | Online Article Text |
id | pubmed-4152321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41523212014-09-05 Expression of THOP1 and Its Relationship to Prognosis in Non-Small Cell Lung Cancer Qi, Lei Li, Shu-hai Si, Li-bo Lu, Ming Tian, Hui PLoS One Research Article BACKGROUND: The study was designed to detect the expression level of thimet oligopeptidase (THOP1) protein in non-small cell lung cancer (NSCLC) and investigate its correlation with clinicopathologic features and prognosis. METHODS: Immunohistochemical staining was used to determine the expression of THOP1 protein in 120 NSCLC specimens and 53 distant normal lung tissues. Quantitative real-time PCR and western blotting were employed to measure the expression of THOP1 in 16 pairs of primary NSCLC and corresponding normal tissues. RESULTS: Analysis of immunohistochemical staining suggested low THOP1 expression was found in 71 (59.2%) of the 120 NSCLC specimens and significantly correlated with positive lymph node metastasis (P = 0.048). However, low THOP1 expression was found in 22 (41.5%) of the 53 normal lung tissues. Chi-square test suggested that the expression of THOP1 was significantly higher in the normal lung tissues than that in the NSCLC specimens (P = 0.032). Real-Time PCR and western blotting showed that NSCLC specimens had decreased THOP1 mRNA and protein expression compared to corresponding normal tissues. Univariate analysis demonstrated that low THOP1 expression significantly predicted decreased 5-year disease-free survival (P = 0.038) and overall survival (P = 0.017). In addition, positive lymph node metastasis (P = 0.025) and advanced TNM stage (P = 0.009) significantly predicted decreased 5-year overall survival. However, multivariate Cox regression analysis showed that only low THOP1 expression retained its significance as an independent prognostic factor for unfavorable 5-year disease-free survival (P = 0.046) and overall survival (P = 0.021). CONCLUSIONS: THOP1 may have clinical potentials to be employed as a promising biomarker to identify individuals with better prognosis and a novel antitumor agent for therapy of patients with NSCLC. Public Library of Science 2014-09-02 /pmc/articles/PMC4152321/ /pubmed/25180910 http://dx.doi.org/10.1371/journal.pone.0106665 Text en © 2014 Qi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Qi, Lei Li, Shu-hai Si, Li-bo Lu, Ming Tian, Hui Expression of THOP1 and Its Relationship to Prognosis in Non-Small Cell Lung Cancer |
title | Expression of THOP1 and Its Relationship to Prognosis in Non-Small Cell Lung Cancer |
title_full | Expression of THOP1 and Its Relationship to Prognosis in Non-Small Cell Lung Cancer |
title_fullStr | Expression of THOP1 and Its Relationship to Prognosis in Non-Small Cell Lung Cancer |
title_full_unstemmed | Expression of THOP1 and Its Relationship to Prognosis in Non-Small Cell Lung Cancer |
title_short | Expression of THOP1 and Its Relationship to Prognosis in Non-Small Cell Lung Cancer |
title_sort | expression of thop1 and its relationship to prognosis in non-small cell lung cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4152321/ https://www.ncbi.nlm.nih.gov/pubmed/25180910 http://dx.doi.org/10.1371/journal.pone.0106665 |
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