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Skin corrosion and irritation test of sunscreen nanoparticles using reconstructed 3D human skin model
OBJECTIVES: Effects of nanoparticles including zinc oxide nanoparticles, titanium oxide nanoparticles, and their mixtures on skin corrosion and irritation were investigated by using in vitro 3D human skin models (KeraSkin((TM))) and the results were compared to those of an in vivo animal test. METHO...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Environmental Health and Toxicology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4152941/ https://www.ncbi.nlm.nih.gov/pubmed/25116366 http://dx.doi.org/10.5620/eht.2014.29.e2014004 |
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author | Choi, Jonghye Kim, Hyejin Choi, Jinhee Oh, Seung Min Park, Jeonggue Park, Kwangsik |
author_facet | Choi, Jonghye Kim, Hyejin Choi, Jinhee Oh, Seung Min Park, Jeonggue Park, Kwangsik |
author_sort | Choi, Jonghye |
collection | PubMed |
description | OBJECTIVES: Effects of nanoparticles including zinc oxide nanoparticles, titanium oxide nanoparticles, and their mixtures on skin corrosion and irritation were investigated by using in vitro 3D human skin models (KeraSkin((TM))) and the results were compared to those of an in vivo animal test. METHODS: Skin models were incubated with nanoparticles for a definite time period and cell viability was measured by the 3-(4, 5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide method. Skin corrosion and irritation were identified by the decreased viability based on the pre-determined threshold. RESULTS: Cell viability after exposure to nanomaterial was not decreased to the pre-determined threshold level, which was 15% after 60 minutes exposure in corrosion test and 50% after 45 minutes exposure in the irritation test. IL-1α release and histopathological findings support the results of cell viability test. In vivo test using rabbits also showed non-corrosive and non-irritant results. CONCLUSIONS: The findings provide the evidence that zinc oxide nanoparticles, titanium oxide nanoparticles and their mixture are ‘non corrosive’ and ‘non-irritant’ to the human skin by a globally harmonized classification system. In vivo test using animals can be replaced by an alternative in vitro test. |
format | Online Article Text |
id | pubmed-4152941 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The Korean Society of Environmental Health and Toxicology |
record_format | MEDLINE/PubMed |
spelling | pubmed-41529412014-09-03 Skin corrosion and irritation test of sunscreen nanoparticles using reconstructed 3D human skin model Choi, Jonghye Kim, Hyejin Choi, Jinhee Oh, Seung Min Park, Jeonggue Park, Kwangsik Environ Health Toxicol Original Article OBJECTIVES: Effects of nanoparticles including zinc oxide nanoparticles, titanium oxide nanoparticles, and their mixtures on skin corrosion and irritation were investigated by using in vitro 3D human skin models (KeraSkin((TM))) and the results were compared to those of an in vivo animal test. METHODS: Skin models were incubated with nanoparticles for a definite time period and cell viability was measured by the 3-(4, 5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide method. Skin corrosion and irritation were identified by the decreased viability based on the pre-determined threshold. RESULTS: Cell viability after exposure to nanomaterial was not decreased to the pre-determined threshold level, which was 15% after 60 minutes exposure in corrosion test and 50% after 45 minutes exposure in the irritation test. IL-1α release and histopathological findings support the results of cell viability test. In vivo test using rabbits also showed non-corrosive and non-irritant results. CONCLUSIONS: The findings provide the evidence that zinc oxide nanoparticles, titanium oxide nanoparticles and their mixture are ‘non corrosive’ and ‘non-irritant’ to the human skin by a globally harmonized classification system. In vivo test using animals can be replaced by an alternative in vitro test. The Korean Society of Environmental Health and Toxicology 2014-07-21 /pmc/articles/PMC4152941/ /pubmed/25116366 http://dx.doi.org/10.5620/eht.2014.29.e2014004 Text en Copyright © 2014 The Korean Society of Environmental Health and Toxicology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Choi, Jonghye Kim, Hyejin Choi, Jinhee Oh, Seung Min Park, Jeonggue Park, Kwangsik Skin corrosion and irritation test of sunscreen nanoparticles using reconstructed 3D human skin model |
title | Skin corrosion and irritation test of sunscreen nanoparticles using reconstructed 3D human skin model |
title_full | Skin corrosion and irritation test of sunscreen nanoparticles using reconstructed 3D human skin model |
title_fullStr | Skin corrosion and irritation test of sunscreen nanoparticles using reconstructed 3D human skin model |
title_full_unstemmed | Skin corrosion and irritation test of sunscreen nanoparticles using reconstructed 3D human skin model |
title_short | Skin corrosion and irritation test of sunscreen nanoparticles using reconstructed 3D human skin model |
title_sort | skin corrosion and irritation test of sunscreen nanoparticles using reconstructed 3d human skin model |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4152941/ https://www.ncbi.nlm.nih.gov/pubmed/25116366 http://dx.doi.org/10.5620/eht.2014.29.e2014004 |
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