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Linking DNRA community structure and activity in a shallow lagoonal estuarine system
Dissimilatory nitrate reduction to ammonium (DNRA) and denitrification are two nitrate respiration pathways in the microbial nitrogen cycle. Diversity and abundance of denitrifying bacteria have been extensively examined in various ecosystems. However, studies on DNRA bacterial diversity are limited...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153293/ https://www.ncbi.nlm.nih.gov/pubmed/25232351 http://dx.doi.org/10.3389/fmicb.2014.00460 |
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author | Song, Bongkeun Lisa, Jessica A. Tobias, Craig R. |
author_facet | Song, Bongkeun Lisa, Jessica A. Tobias, Craig R. |
author_sort | Song, Bongkeun |
collection | PubMed |
description | Dissimilatory nitrate reduction to ammonium (DNRA) and denitrification are two nitrate respiration pathways in the microbial nitrogen cycle. Diversity and abundance of denitrifying bacteria have been extensively examined in various ecosystems. However, studies on DNRA bacterial diversity are limited, and the linkage between the structure and activity of DNRA communities has yet to be discovered. We examined the composition, diversity, abundance, and activities of DNRA communities at five sites along a salinity gradient in the New River Estuary, North Carolina, USA, a shallow temporal/lagoonal estuarine system. Sediment slurry incubation experiments with (15)N-nitrate were conducted to measure potential DNRA rates, while the abundance of DNRA communities was calculated using quantitative PCR of nrfA genes encoding cytochrome C nitrite reductase, commonly found in DNRA bacteria. A pyrosequencing method targeting nrfA genes was developed using an Ion Torrent sequencer to examine the diversity and composition of DNRA communities within the estuarine sediment community. We found higher levels of nrfA gene abundance and DNRA activities in sediments with higher percent organic content. Pyrosequencing analysis of nrfA genes revealed spatial variation of DNRA communities along the salinity gradient of the New River Estuary. Percent abundance of dominant populations was found to have significant influence on overall activities of DNRA communities. Abundance of dominant DNRA bacteria and organic carbon availability are important regulators of DNRA activities in the eutrophic New River Estuary. |
format | Online Article Text |
id | pubmed-4153293 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-41532932014-09-17 Linking DNRA community structure and activity in a shallow lagoonal estuarine system Song, Bongkeun Lisa, Jessica A. Tobias, Craig R. Front Microbiol Microbiology Dissimilatory nitrate reduction to ammonium (DNRA) and denitrification are two nitrate respiration pathways in the microbial nitrogen cycle. Diversity and abundance of denitrifying bacteria have been extensively examined in various ecosystems. However, studies on DNRA bacterial diversity are limited, and the linkage between the structure and activity of DNRA communities has yet to be discovered. We examined the composition, diversity, abundance, and activities of DNRA communities at five sites along a salinity gradient in the New River Estuary, North Carolina, USA, a shallow temporal/lagoonal estuarine system. Sediment slurry incubation experiments with (15)N-nitrate were conducted to measure potential DNRA rates, while the abundance of DNRA communities was calculated using quantitative PCR of nrfA genes encoding cytochrome C nitrite reductase, commonly found in DNRA bacteria. A pyrosequencing method targeting nrfA genes was developed using an Ion Torrent sequencer to examine the diversity and composition of DNRA communities within the estuarine sediment community. We found higher levels of nrfA gene abundance and DNRA activities in sediments with higher percent organic content. Pyrosequencing analysis of nrfA genes revealed spatial variation of DNRA communities along the salinity gradient of the New River Estuary. Percent abundance of dominant populations was found to have significant influence on overall activities of DNRA communities. Abundance of dominant DNRA bacteria and organic carbon availability are important regulators of DNRA activities in the eutrophic New River Estuary. Frontiers Media S.A. 2014-09-03 /pmc/articles/PMC4153293/ /pubmed/25232351 http://dx.doi.org/10.3389/fmicb.2014.00460 Text en Copyright © 2014 Song, Lisa and Tobias. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Song, Bongkeun Lisa, Jessica A. Tobias, Craig R. Linking DNRA community structure and activity in a shallow lagoonal estuarine system |
title | Linking DNRA community structure and activity in a shallow lagoonal estuarine system |
title_full | Linking DNRA community structure and activity in a shallow lagoonal estuarine system |
title_fullStr | Linking DNRA community structure and activity in a shallow lagoonal estuarine system |
title_full_unstemmed | Linking DNRA community structure and activity in a shallow lagoonal estuarine system |
title_short | Linking DNRA community structure and activity in a shallow lagoonal estuarine system |
title_sort | linking dnra community structure and activity in a shallow lagoonal estuarine system |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153293/ https://www.ncbi.nlm.nih.gov/pubmed/25232351 http://dx.doi.org/10.3389/fmicb.2014.00460 |
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