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Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies
BACKGROUND: With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples o...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153446/ https://www.ncbi.nlm.nih.gov/pubmed/25141858 http://dx.doi.org/10.1186/1472-6750-14-78 |
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author | Sharma, Harshita Kinoshita, Yasunori Fujiu, Seiichi Nomura, Shota Sawada, Mizuho Ahmed, Shamim Shibuya, Masaki Shirai, Kosaku Takamatsu, Syota Watanabe, Tsuyoshi Yamazaki, Hitoshi Kamiyama, Ryohei Kobayashi, Tetsuya Arai, Hidenao Suzuki, Miho Nemoto, Naoto Ando, Ki Uchida, Hidekazu Kitamura, Koichiro Takei, Osamu Nishigaki, Koichi |
author_facet | Sharma, Harshita Kinoshita, Yasunori Fujiu, Seiichi Nomura, Shota Sawada, Mizuho Ahmed, Shamim Shibuya, Masaki Shirai, Kosaku Takamatsu, Syota Watanabe, Tsuyoshi Yamazaki, Hitoshi Kamiyama, Ryohei Kobayashi, Tetsuya Arai, Hidenao Suzuki, Miho Nemoto, Naoto Ando, Ki Uchida, Hidekazu Kitamura, Koichiro Takei, Osamu Nishigaki, Koichi |
author_sort | Sharma, Harshita |
collection | PubMed |
description | BACKGROUND: With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip. RESULTS: In the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications. CONCLUSIONS: The reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies. |
format | Online Article Text |
id | pubmed-4153446 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41534462014-09-12 Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies Sharma, Harshita Kinoshita, Yasunori Fujiu, Seiichi Nomura, Shota Sawada, Mizuho Ahmed, Shamim Shibuya, Masaki Shirai, Kosaku Takamatsu, Syota Watanabe, Tsuyoshi Yamazaki, Hitoshi Kamiyama, Ryohei Kobayashi, Tetsuya Arai, Hidenao Suzuki, Miho Nemoto, Naoto Ando, Ki Uchida, Hidekazu Kitamura, Koichiro Takei, Osamu Nishigaki, Koichi BMC Biotechnol Methodology Article BACKGROUND: With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip. RESULTS: In the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications. CONCLUSIONS: The reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies. BioMed Central 2014-08-21 /pmc/articles/PMC4153446/ /pubmed/25141858 http://dx.doi.org/10.1186/1472-6750-14-78 Text en Copyright © 2014 Sharma et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Methodology Article Sharma, Harshita Kinoshita, Yasunori Fujiu, Seiichi Nomura, Shota Sawada, Mizuho Ahmed, Shamim Shibuya, Masaki Shirai, Kosaku Takamatsu, Syota Watanabe, Tsuyoshi Yamazaki, Hitoshi Kamiyama, Ryohei Kobayashi, Tetsuya Arai, Hidenao Suzuki, Miho Nemoto, Naoto Ando, Ki Uchida, Hidekazu Kitamura, Koichiro Takei, Osamu Nishigaki, Koichi Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies |
title | Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies |
title_full | Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies |
title_fullStr | Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies |
title_full_unstemmed | Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies |
title_short | Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies |
title_sort | establishment of a reborn mmv-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies |
topic | Methodology Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153446/ https://www.ncbi.nlm.nih.gov/pubmed/25141858 http://dx.doi.org/10.1186/1472-6750-14-78 |
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