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Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies

BACKGROUND: With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples o...

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Autores principales: Sharma, Harshita, Kinoshita, Yasunori, Fujiu, Seiichi, Nomura, Shota, Sawada, Mizuho, Ahmed, Shamim, Shibuya, Masaki, Shirai, Kosaku, Takamatsu, Syota, Watanabe, Tsuyoshi, Yamazaki, Hitoshi, Kamiyama, Ryohei, Kobayashi, Tetsuya, Arai, Hidenao, Suzuki, Miho, Nemoto, Naoto, Ando, Ki, Uchida, Hidekazu, Kitamura, Koichiro, Takei, Osamu, Nishigaki, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153446/
https://www.ncbi.nlm.nih.gov/pubmed/25141858
http://dx.doi.org/10.1186/1472-6750-14-78
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author Sharma, Harshita
Kinoshita, Yasunori
Fujiu, Seiichi
Nomura, Shota
Sawada, Mizuho
Ahmed, Shamim
Shibuya, Masaki
Shirai, Kosaku
Takamatsu, Syota
Watanabe, Tsuyoshi
Yamazaki, Hitoshi
Kamiyama, Ryohei
Kobayashi, Tetsuya
Arai, Hidenao
Suzuki, Miho
Nemoto, Naoto
Ando, Ki
Uchida, Hidekazu
Kitamura, Koichiro
Takei, Osamu
Nishigaki, Koichi
author_facet Sharma, Harshita
Kinoshita, Yasunori
Fujiu, Seiichi
Nomura, Shota
Sawada, Mizuho
Ahmed, Shamim
Shibuya, Masaki
Shirai, Kosaku
Takamatsu, Syota
Watanabe, Tsuyoshi
Yamazaki, Hitoshi
Kamiyama, Ryohei
Kobayashi, Tetsuya
Arai, Hidenao
Suzuki, Miho
Nemoto, Naoto
Ando, Ki
Uchida, Hidekazu
Kitamura, Koichiro
Takei, Osamu
Nishigaki, Koichi
author_sort Sharma, Harshita
collection PubMed
description BACKGROUND: With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip. RESULTS: In the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications. CONCLUSIONS: The reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies.
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spelling pubmed-41534462014-09-12 Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies Sharma, Harshita Kinoshita, Yasunori Fujiu, Seiichi Nomura, Shota Sawada, Mizuho Ahmed, Shamim Shibuya, Masaki Shirai, Kosaku Takamatsu, Syota Watanabe, Tsuyoshi Yamazaki, Hitoshi Kamiyama, Ryohei Kobayashi, Tetsuya Arai, Hidenao Suzuki, Miho Nemoto, Naoto Ando, Ki Uchida, Hidekazu Kitamura, Koichiro Takei, Osamu Nishigaki, Koichi BMC Biotechnol Methodology Article BACKGROUND: With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip. RESULTS: In the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications. CONCLUSIONS: The reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies. BioMed Central 2014-08-21 /pmc/articles/PMC4153446/ /pubmed/25141858 http://dx.doi.org/10.1186/1472-6750-14-78 Text en Copyright © 2014 Sharma et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Methodology Article
Sharma, Harshita
Kinoshita, Yasunori
Fujiu, Seiichi
Nomura, Shota
Sawada, Mizuho
Ahmed, Shamim
Shibuya, Masaki
Shirai, Kosaku
Takamatsu, Syota
Watanabe, Tsuyoshi
Yamazaki, Hitoshi
Kamiyama, Ryohei
Kobayashi, Tetsuya
Arai, Hidenao
Suzuki, Miho
Nemoto, Naoto
Ando, Ki
Uchida, Hidekazu
Kitamura, Koichiro
Takei, Osamu
Nishigaki, Koichi
Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies
title Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies
title_full Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies
title_fullStr Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies
title_full_unstemmed Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies
title_short Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies
title_sort establishment of a reborn mmv-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153446/
https://www.ncbi.nlm.nih.gov/pubmed/25141858
http://dx.doi.org/10.1186/1472-6750-14-78
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