Cargando…

Effects of Surface Asymmetry on Neuronal Growth

Detailed knowledge of how the surface physical properties, such as mechanics, topography and texture influence axonal outgrowth and guidance is essential for understanding the processes that control neuron development, the formation of functional neuronal connections and nerve regeneration. Here we...

Descripción completa

Detalles Bibliográficos
Autores principales: Spedden, Elise, Wiens, Matthew R., Demirel, Melik C., Staii, Cristian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153665/
https://www.ncbi.nlm.nih.gov/pubmed/25184796
http://dx.doi.org/10.1371/journal.pone.0106709
_version_ 1782333322794369024
author Spedden, Elise
Wiens, Matthew R.
Demirel, Melik C.
Staii, Cristian
author_facet Spedden, Elise
Wiens, Matthew R.
Demirel, Melik C.
Staii, Cristian
author_sort Spedden, Elise
collection PubMed
description Detailed knowledge of how the surface physical properties, such as mechanics, topography and texture influence axonal outgrowth and guidance is essential for understanding the processes that control neuron development, the formation of functional neuronal connections and nerve regeneration. Here we synthesize asymmetric surfaces with well-controlled topography and texture and perform a systematic experimental and theoretical investigation of axonal outgrowth on these substrates. We demonstrate unidirectional axonal bias imparted by the surface ratchet-based topography and quantify the topographical guidance cues that control neuronal growth. We describe the growth cone dynamics using a general stochastic model (Fokker-Planck formalism) and use this model to extract two key dynamical parameters: diffusion (cell motility) coefficient and asymmetric drift coefficient. The drift coefficient is identified with the torque caused by the asymmetric ratchet topography. We relate the observed directional bias in axonal outgrowth to cellular contact guidance behavior, which results in an increase in the cell-surface coupling with increased surface anisotropy. We also demonstrate that the disruption of cytoskeletal dynamics through application of Taxol (stabilizer of microtubules) and Blebbistatin (inhibitor of myosin II activity) greatly reduces the directional bias imparted by these asymmetric surfaces. These results provide new insight into the role played by topographical cues in neuronal growth and could lead to new methods for stimulating neuronal regeneration and the engineering of artificial neuronal tissue.
format Online
Article
Text
id pubmed-4153665
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-41536652014-09-05 Effects of Surface Asymmetry on Neuronal Growth Spedden, Elise Wiens, Matthew R. Demirel, Melik C. Staii, Cristian PLoS One Research Article Detailed knowledge of how the surface physical properties, such as mechanics, topography and texture influence axonal outgrowth and guidance is essential for understanding the processes that control neuron development, the formation of functional neuronal connections and nerve regeneration. Here we synthesize asymmetric surfaces with well-controlled topography and texture and perform a systematic experimental and theoretical investigation of axonal outgrowth on these substrates. We demonstrate unidirectional axonal bias imparted by the surface ratchet-based topography and quantify the topographical guidance cues that control neuronal growth. We describe the growth cone dynamics using a general stochastic model (Fokker-Planck formalism) and use this model to extract two key dynamical parameters: diffusion (cell motility) coefficient and asymmetric drift coefficient. The drift coefficient is identified with the torque caused by the asymmetric ratchet topography. We relate the observed directional bias in axonal outgrowth to cellular contact guidance behavior, which results in an increase in the cell-surface coupling with increased surface anisotropy. We also demonstrate that the disruption of cytoskeletal dynamics through application of Taxol (stabilizer of microtubules) and Blebbistatin (inhibitor of myosin II activity) greatly reduces the directional bias imparted by these asymmetric surfaces. These results provide new insight into the role played by topographical cues in neuronal growth and could lead to new methods for stimulating neuronal regeneration and the engineering of artificial neuronal tissue. Public Library of Science 2014-09-03 /pmc/articles/PMC4153665/ /pubmed/25184796 http://dx.doi.org/10.1371/journal.pone.0106709 Text en © 2014 Spedden et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Spedden, Elise
Wiens, Matthew R.
Demirel, Melik C.
Staii, Cristian
Effects of Surface Asymmetry on Neuronal Growth
title Effects of Surface Asymmetry on Neuronal Growth
title_full Effects of Surface Asymmetry on Neuronal Growth
title_fullStr Effects of Surface Asymmetry on Neuronal Growth
title_full_unstemmed Effects of Surface Asymmetry on Neuronal Growth
title_short Effects of Surface Asymmetry on Neuronal Growth
title_sort effects of surface asymmetry on neuronal growth
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153665/
https://www.ncbi.nlm.nih.gov/pubmed/25184796
http://dx.doi.org/10.1371/journal.pone.0106709
work_keys_str_mv AT speddenelise effectsofsurfaceasymmetryonneuronalgrowth
AT wiensmatthewr effectsofsurfaceasymmetryonneuronalgrowth
AT demirelmelikc effectsofsurfaceasymmetryonneuronalgrowth
AT staiicristian effectsofsurfaceasymmetryonneuronalgrowth