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Modulation of Cerrena unicolor laccase and manganese peroxidase production
Among seven carbon sources tested, glycerol and glucose favored the Cerrena unicolor laccase production (18.8-20.3 U/mL); in addition, glycerol ensured the highest manganese peroxidase (MnP) activity (2 U/mL). Substitution of glycerol with the ethanol production residue (EPR) gave the highest laccas...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153879/ https://www.ncbi.nlm.nih.gov/pubmed/25191637 http://dx.doi.org/10.1186/2193-1801-3-463 |
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author | Kachlishvili, Eva Metreveli, Eka Elisashvili, Vladimir |
author_facet | Kachlishvili, Eva Metreveli, Eka Elisashvili, Vladimir |
author_sort | Kachlishvili, Eva |
collection | PubMed |
description | Among seven carbon sources tested, glycerol and glucose favored the Cerrena unicolor laccase production (18.8-20.3 U/mL); in addition, glycerol ensured the highest manganese peroxidase (MnP) activity (2 U/mL). Substitution of glycerol with the ethanol production residue (EPR) gave the highest laccase (90.1 U/mL) activity, while the walnut pericarp provided the highest MnP activity (7.4 U/mL). Supplementation of medium with 1 mM copper and 1 mM xylidine at appropriate time caused significant additive effect on laccase expression (333.2 U/mL) in shake-flask experiments. Overproduction of laccase activity (507 U/mL) and secretion of MnP activity was obtained when C. unicolor was cultivated in stirred-tank fermenter. C. unicolor showed several distinctive and attractive technological features: it is capable to synthesize high levels of oxidases under high carbon and high nitrogen conditions and it secretes high laccase activity during trophophase. |
format | Online Article Text |
id | pubmed-4153879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-41538792014-09-04 Modulation of Cerrena unicolor laccase and manganese peroxidase production Kachlishvili, Eva Metreveli, Eka Elisashvili, Vladimir Springerplus Research Among seven carbon sources tested, glycerol and glucose favored the Cerrena unicolor laccase production (18.8-20.3 U/mL); in addition, glycerol ensured the highest manganese peroxidase (MnP) activity (2 U/mL). Substitution of glycerol with the ethanol production residue (EPR) gave the highest laccase (90.1 U/mL) activity, while the walnut pericarp provided the highest MnP activity (7.4 U/mL). Supplementation of medium with 1 mM copper and 1 mM xylidine at appropriate time caused significant additive effect on laccase expression (333.2 U/mL) in shake-flask experiments. Overproduction of laccase activity (507 U/mL) and secretion of MnP activity was obtained when C. unicolor was cultivated in stirred-tank fermenter. C. unicolor showed several distinctive and attractive technological features: it is capable to synthesize high levels of oxidases under high carbon and high nitrogen conditions and it secretes high laccase activity during trophophase. Springer International Publishing 2014-08-26 /pmc/articles/PMC4153879/ /pubmed/25191637 http://dx.doi.org/10.1186/2193-1801-3-463 Text en © Kachlishvili et al.; licensee Springer. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
spellingShingle | Research Kachlishvili, Eva Metreveli, Eka Elisashvili, Vladimir Modulation of Cerrena unicolor laccase and manganese peroxidase production |
title | Modulation of Cerrena unicolor laccase and manganese peroxidase production |
title_full | Modulation of Cerrena unicolor laccase and manganese peroxidase production |
title_fullStr | Modulation of Cerrena unicolor laccase and manganese peroxidase production |
title_full_unstemmed | Modulation of Cerrena unicolor laccase and manganese peroxidase production |
title_short | Modulation of Cerrena unicolor laccase and manganese peroxidase production |
title_sort | modulation of cerrena unicolor laccase and manganese peroxidase production |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153879/ https://www.ncbi.nlm.nih.gov/pubmed/25191637 http://dx.doi.org/10.1186/2193-1801-3-463 |
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