Ultra Scale-Down Characterization of the Impact of Conditioning Methods for Harvested Cell Broths on Clarification by Continuous Centrifugation—Recovery of Domain Antibodies from rec E. coli

The processing of harvested E. coli cell broths is examined where the expressed protein product has been released into the extracellular space. Pre-treatment methods such as freeze–thaw, flocculation, and homogenization are studied. The resultant suspensions are characterized in terms of the particl...

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Detalles Bibliográficos
Autores principales: Chatel, Alex, Kumpalume, Peter, Hoare, Mike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2014
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153950/
https://www.ncbi.nlm.nih.gov/pubmed/24284936
http://dx.doi.org/10.1002/bit.25164

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153950/
https://www.ncbi.nlm.nih.gov/pubmed/24284936
http://dx.doi.org/10.1002/bit.25164

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