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Ultra Scale-Down Characterization of the Impact of Conditioning Methods for Harvested Cell Broths on Clarification by Continuous Centrifugation—Recovery of Domain Antibodies from rec E. coli
The processing of harvested E. coli cell broths is examined where the expressed protein product has been released into the extracellular space. Pre-treatment methods such as freeze–thaw, flocculation, and homogenization are studied. The resultant suspensions are characterized in terms of the particl...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Blackwell Publishing Ltd
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153950/ https://www.ncbi.nlm.nih.gov/pubmed/24284936 http://dx.doi.org/10.1002/bit.25164 |