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MRC5 and QU-DB bystander cells can produce bystander factors and induce radiation bystander effect

Radiation damages initiated by radiation-induced bystander effect (RIBE) are not limited to the first or immediate neighbors of the irradiated cells, but the effects have been observed in the cells far from the irradiation site. It has been postulated that bystander cells, by producing bystander fac...

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Autores principales: Toossi, Mohammad Taghi Bahreyni, Mohebbi, Shokoufeh, Samani, Roghayeh Kamran, Soleymanifard, Shokouhozaman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4154187/
https://www.ncbi.nlm.nih.gov/pubmed/25190998
http://dx.doi.org/10.4103/0971-6203.139011
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author Toossi, Mohammad Taghi Bahreyni
Mohebbi, Shokoufeh
Samani, Roghayeh Kamran
Soleymanifard, Shokouhozaman
author_facet Toossi, Mohammad Taghi Bahreyni
Mohebbi, Shokoufeh
Samani, Roghayeh Kamran
Soleymanifard, Shokouhozaman
author_sort Toossi, Mohammad Taghi Bahreyni
collection PubMed
description Radiation damages initiated by radiation-induced bystander effect (RIBE) are not limited to the first or immediate neighbors of the irradiated cells, but the effects have been observed in the cells far from the irradiation site. It has been postulated that bystander cells, by producing bystander factors, are actively involved in the propagation of bystander effect in the regions beyond the initial irradiated site. Current study was planned to test the hypothesis. MRC5 and QU-DB cell lines were irradiated, and successive medium transfer technique was performed to induce bystander effects in two bystander cell groups. Conditioned medium extracted from the target cells was transferred to the bystander cells (first bystander cells). After one hour, conditioned medium was substituted by fresh medium. Two hours later, the fresh medium was transferred to a second group of non-irradiated cells (second bystander cells). Micronucleated cells (MC) were counted to quantify damages induced in the first and second bystander cell groups. Radiation effect was observed in the second bystander cells as well as in the first ones. Statistical analyses revealed that the number of MC in second bystander subgroups was significantly more than the corresponding value observed in control groups, but in most cases it was equal to the number of MC observed in the first bystander cells. MRC5 and QU-DB bystander cells can produce and release bystander signals in the culture medium and affect non-irradiated cells. Therefore, they may contribute to the RIBE propagation.
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spelling pubmed-41541872014-09-04 MRC5 and QU-DB bystander cells can produce bystander factors and induce radiation bystander effect Toossi, Mohammad Taghi Bahreyni Mohebbi, Shokoufeh Samani, Roghayeh Kamran Soleymanifard, Shokouhozaman J Med Phys Original Article Radiation damages initiated by radiation-induced bystander effect (RIBE) are not limited to the first or immediate neighbors of the irradiated cells, but the effects have been observed in the cells far from the irradiation site. It has been postulated that bystander cells, by producing bystander factors, are actively involved in the propagation of bystander effect in the regions beyond the initial irradiated site. Current study was planned to test the hypothesis. MRC5 and QU-DB cell lines were irradiated, and successive medium transfer technique was performed to induce bystander effects in two bystander cell groups. Conditioned medium extracted from the target cells was transferred to the bystander cells (first bystander cells). After one hour, conditioned medium was substituted by fresh medium. Two hours later, the fresh medium was transferred to a second group of non-irradiated cells (second bystander cells). Micronucleated cells (MC) were counted to quantify damages induced in the first and second bystander cell groups. Radiation effect was observed in the second bystander cells as well as in the first ones. Statistical analyses revealed that the number of MC in second bystander subgroups was significantly more than the corresponding value observed in control groups, but in most cases it was equal to the number of MC observed in the first bystander cells. MRC5 and QU-DB bystander cells can produce and release bystander signals in the culture medium and affect non-irradiated cells. Therefore, they may contribute to the RIBE propagation. Medknow Publications & Media Pvt Ltd 2014 /pmc/articles/PMC4154187/ /pubmed/25190998 http://dx.doi.org/10.4103/0971-6203.139011 Text en Copyright: © Journal of Medical Physics http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Toossi, Mohammad Taghi Bahreyni
Mohebbi, Shokoufeh
Samani, Roghayeh Kamran
Soleymanifard, Shokouhozaman
MRC5 and QU-DB bystander cells can produce bystander factors and induce radiation bystander effect
title MRC5 and QU-DB bystander cells can produce bystander factors and induce radiation bystander effect
title_full MRC5 and QU-DB bystander cells can produce bystander factors and induce radiation bystander effect
title_fullStr MRC5 and QU-DB bystander cells can produce bystander factors and induce radiation bystander effect
title_full_unstemmed MRC5 and QU-DB bystander cells can produce bystander factors and induce radiation bystander effect
title_short MRC5 and QU-DB bystander cells can produce bystander factors and induce radiation bystander effect
title_sort mrc5 and qu-db bystander cells can produce bystander factors and induce radiation bystander effect
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4154187/
https://www.ncbi.nlm.nih.gov/pubmed/25190998
http://dx.doi.org/10.4103/0971-6203.139011
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