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Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation

Amidst growing concerns over the spread of antibiotic-resistant Staphylococcus aureus strains, the identification of alternative therapeutic molecules has become paramount. Previously, we utilized a Caenorhabditis elegans–S. aureus screening platform to identify potential anti-infective agents from...

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Detalles Bibliográficos
Autores principales: Kong, Cin, Tan, Man-Wah, Nathan, Sheila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4154301/
https://www.ncbi.nlm.nih.gov/pubmed/24972867
http://dx.doi.org/10.1242/bio.20148334
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author Kong, Cin
Tan, Man-Wah
Nathan, Sheila
author_facet Kong, Cin
Tan, Man-Wah
Nathan, Sheila
author_sort Kong, Cin
collection PubMed
description Amidst growing concerns over the spread of antibiotic-resistant Staphylococcus aureus strains, the identification of alternative therapeutic molecules has become paramount. Previously, we utilized a Caenorhabditis elegans–S. aureus screening platform to identify potential anti-infective agents from a collection of natural extracts and synthetic compounds. One of the hits obtained from the screen was the aqueous extract of Orthosiphon stamineus leaves (UE-12) that enhanced the survival of infected nematodes without interfering with bacterial growth. In this study, we used a fluorescent transgenic reporter strain and observed that the repressed expression of the lys-7 defense gene in infected nematodes was restored in the presence of UE-12. Analysis of a selected panel of PMK-1 and DAF-16-regulated transcripts and loss-of-function mutants in these pathways indicates that the protective role of UE-12 is mediated via the p38 MAP kinase and insulin-like signaling pathways. Further analysis of a panel of known bioactive compounds of UE-12 proposed eupatorin (C(18)H(16)O(7)) as the possible candidate active molecule contributing to the anti-infective property of UE-12. Taken together, these findings strongly suggest that the O. stamineus leaf extract is a promising anti-infective agent that confers an advantage in survival against S. aureus infection by modulating the immune response of the infected host.
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spelling pubmed-41543012014-09-04 Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation Kong, Cin Tan, Man-Wah Nathan, Sheila Biol Open Research Article Amidst growing concerns over the spread of antibiotic-resistant Staphylococcus aureus strains, the identification of alternative therapeutic molecules has become paramount. Previously, we utilized a Caenorhabditis elegans–S. aureus screening platform to identify potential anti-infective agents from a collection of natural extracts and synthetic compounds. One of the hits obtained from the screen was the aqueous extract of Orthosiphon stamineus leaves (UE-12) that enhanced the survival of infected nematodes without interfering with bacterial growth. In this study, we used a fluorescent transgenic reporter strain and observed that the repressed expression of the lys-7 defense gene in infected nematodes was restored in the presence of UE-12. Analysis of a selected panel of PMK-1 and DAF-16-regulated transcripts and loss-of-function mutants in these pathways indicates that the protective role of UE-12 is mediated via the p38 MAP kinase and insulin-like signaling pathways. Further analysis of a panel of known bioactive compounds of UE-12 proposed eupatorin (C(18)H(16)O(7)) as the possible candidate active molecule contributing to the anti-infective property of UE-12. Taken together, these findings strongly suggest that the O. stamineus leaf extract is a promising anti-infective agent that confers an advantage in survival against S. aureus infection by modulating the immune response of the infected host. The Company of Biologists 2014-06-27 /pmc/articles/PMC4154301/ /pubmed/24972867 http://dx.doi.org/10.1242/bio.20148334 Text en © 2014. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Kong, Cin
Tan, Man-Wah
Nathan, Sheila
Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation
title Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation
title_full Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation
title_fullStr Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation
title_full_unstemmed Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation
title_short Orthosiphon stamineus protects Caenorhabditis elegans against Staphylococcus aureus infection through immunomodulation
title_sort orthosiphon stamineus protects caenorhabditis elegans against staphylococcus aureus infection through immunomodulation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4154301/
https://www.ncbi.nlm.nih.gov/pubmed/24972867
http://dx.doi.org/10.1242/bio.20148334
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