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Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers

Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker s...

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Autores principales: Cichorz, Sandra, Gośka, Maria, Litwiniec, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155165/
https://www.ncbi.nlm.nih.gov/pubmed/24880640
http://dx.doi.org/10.1007/s12033-014-9770-0
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author Cichorz, Sandra
Gośka, Maria
Litwiniec, Anna
author_facet Cichorz, Sandra
Gośka, Maria
Litwiniec, Anna
author_sort Cichorz, Sandra
collection PubMed
description Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker systems such as: inter simple sequence repeats (ISSRs), random amplified polymorphic DNA (RAPD), and by estimation of ploidy level based on flow cytometry. As a result, only one ISSR primer (ISSR1) and three RAPD primers (RAPD1, RAPD2, RAPD4) were required to identify all genotypes. Moreover, the use of the above mentioned molecular markers enable the proper species recognition of the interspecific hybrid M. × giganteus “Floridulus,” which has been previously mislabeled as M. floridulus. The highest genetic similarity coefficient (0.94) was observed between M. × giganteus clones, which indicates that the genetic diversity within this species was very low. Whereas M. sinensis genotypes represented a relatively wide diversity with similarity coefficient of 0.58. Cluster analysis using UPGMA grouped the 18 accessions in three clusters according to species affiliation including relabeled M. × giganteus “Floridulus,” which proved to be closely related to M.  × giganteus. Similar groupings were evident in the PCoA analysis.
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spelling pubmed-41551652014-09-08 Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers Cichorz, Sandra Gośka, Maria Litwiniec, Anna Mol Biotechnol Research Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker systems such as: inter simple sequence repeats (ISSRs), random amplified polymorphic DNA (RAPD), and by estimation of ploidy level based on flow cytometry. As a result, only one ISSR primer (ISSR1) and three RAPD primers (RAPD1, RAPD2, RAPD4) were required to identify all genotypes. Moreover, the use of the above mentioned molecular markers enable the proper species recognition of the interspecific hybrid M. × giganteus “Floridulus,” which has been previously mislabeled as M. floridulus. The highest genetic similarity coefficient (0.94) was observed between M. × giganteus clones, which indicates that the genetic diversity within this species was very low. Whereas M. sinensis genotypes represented a relatively wide diversity with similarity coefficient of 0.58. Cluster analysis using UPGMA grouped the 18 accessions in three clusters according to species affiliation including relabeled M. × giganteus “Floridulus,” which proved to be closely related to M.  × giganteus. Similar groupings were evident in the PCoA analysis. Springer US 2014-06-01 2014 /pmc/articles/PMC4155165/ /pubmed/24880640 http://dx.doi.org/10.1007/s12033-014-9770-0 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Research
Cichorz, Sandra
Gośka, Maria
Litwiniec, Anna
Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers
title Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers
title_full Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers
title_fullStr Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers
title_full_unstemmed Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers
title_short Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers
title_sort miscanthus: genetic diversity and genotype identification using issr and rapd markers
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155165/
https://www.ncbi.nlm.nih.gov/pubmed/24880640
http://dx.doi.org/10.1007/s12033-014-9770-0
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