Cargando…
Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers
Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker s...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155165/ https://www.ncbi.nlm.nih.gov/pubmed/24880640 http://dx.doi.org/10.1007/s12033-014-9770-0 |
_version_ | 1782333549282590720 |
---|---|
author | Cichorz, Sandra Gośka, Maria Litwiniec, Anna |
author_facet | Cichorz, Sandra Gośka, Maria Litwiniec, Anna |
author_sort | Cichorz, Sandra |
collection | PubMed |
description | Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker systems such as: inter simple sequence repeats (ISSRs), random amplified polymorphic DNA (RAPD), and by estimation of ploidy level based on flow cytometry. As a result, only one ISSR primer (ISSR1) and three RAPD primers (RAPD1, RAPD2, RAPD4) were required to identify all genotypes. Moreover, the use of the above mentioned molecular markers enable the proper species recognition of the interspecific hybrid M. × giganteus “Floridulus,” which has been previously mislabeled as M. floridulus. The highest genetic similarity coefficient (0.94) was observed between M. × giganteus clones, which indicates that the genetic diversity within this species was very low. Whereas M. sinensis genotypes represented a relatively wide diversity with similarity coefficient of 0.58. Cluster analysis using UPGMA grouped the 18 accessions in three clusters according to species affiliation including relabeled M. × giganteus “Floridulus,” which proved to be closely related to M. × giganteus. Similar groupings were evident in the PCoA analysis. |
format | Online Article Text |
id | pubmed-4155165 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-41551652014-09-08 Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers Cichorz, Sandra Gośka, Maria Litwiniec, Anna Mol Biotechnol Research Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker systems such as: inter simple sequence repeats (ISSRs), random amplified polymorphic DNA (RAPD), and by estimation of ploidy level based on flow cytometry. As a result, only one ISSR primer (ISSR1) and three RAPD primers (RAPD1, RAPD2, RAPD4) were required to identify all genotypes. Moreover, the use of the above mentioned molecular markers enable the proper species recognition of the interspecific hybrid M. × giganteus “Floridulus,” which has been previously mislabeled as M. floridulus. The highest genetic similarity coefficient (0.94) was observed between M. × giganteus clones, which indicates that the genetic diversity within this species was very low. Whereas M. sinensis genotypes represented a relatively wide diversity with similarity coefficient of 0.58. Cluster analysis using UPGMA grouped the 18 accessions in three clusters according to species affiliation including relabeled M. × giganteus “Floridulus,” which proved to be closely related to M. × giganteus. Similar groupings were evident in the PCoA analysis. Springer US 2014-06-01 2014 /pmc/articles/PMC4155165/ /pubmed/24880640 http://dx.doi.org/10.1007/s12033-014-9770-0 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Research Cichorz, Sandra Gośka, Maria Litwiniec, Anna Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers |
title | Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers |
title_full | Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers |
title_fullStr | Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers |
title_full_unstemmed | Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers |
title_short | Miscanthus: Genetic Diversity and Genotype Identification Using ISSR and RAPD Markers |
title_sort | miscanthus: genetic diversity and genotype identification using issr and rapd markers |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155165/ https://www.ncbi.nlm.nih.gov/pubmed/24880640 http://dx.doi.org/10.1007/s12033-014-9770-0 |
work_keys_str_mv | AT cichorzsandra miscanthusgeneticdiversityandgenotypeidentificationusingissrandrapdmarkers AT goskamaria miscanthusgeneticdiversityandgenotypeidentificationusingissrandrapdmarkers AT litwiniecanna miscanthusgeneticdiversityandgenotypeidentificationusingissrandrapdmarkers |