Detection of quinolone-resistance mutations of parC gene in clinical isolates of Acinetobacter baumannii in Iran

BACKGROUND: The purpose of this study was to screen of parC gene mutations in clinical isolates of Acinetobacter baumannii from intensive care units (ICUs) of Alzahra Hospital, Isfahan, Iran. MATERIALS AND METHODS: Seventy isolates of A. baumannii between March 2011 and June 2012 were studied. Susce...

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Autores principales: Vakili, Bahareh, Khorvash, Farzin, Fazeli, Hossein, Khaleghi, Moj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155714/
https://www.ncbi.nlm.nih.gov/pubmed/25197301
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author Vakili, Bahareh
Khorvash, Farzin
Fazeli, Hossein
Khaleghi, Moj
author_facet Vakili, Bahareh
Khorvash, Farzin
Fazeli, Hossein
Khaleghi, Moj
author_sort Vakili, Bahareh
collection PubMed
description BACKGROUND: The purpose of this study was to screen of parC gene mutations in clinical isolates of Acinetobacter baumannii from intensive care units (ICUs) of Alzahra Hospital, Isfahan, Iran. MATERIALS AND METHODS: Seventy isolates of A. baumannii between March 2011 and June 2012 were studied. Susceptibility test was established by E-test method. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing was performed for detection of parC gene mutation. RESULTS: 77.1% of specimens were highly resistant. Mutation at position 80 in parC was observed in 93% of isolates. CONCLUSION: High proportion of A. baumannii isolates had a mutation in parC that can play an important role in increased incidence of these isolates.
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spelling pubmed-41557142014-09-05 Detection of quinolone-resistance mutations of parC gene in clinical isolates of Acinetobacter baumannii in Iran Vakili, Bahareh Khorvash, Farzin Fazeli, Hossein Khaleghi, Moj J Res Med Sci Short Communication BACKGROUND: The purpose of this study was to screen of parC gene mutations in clinical isolates of Acinetobacter baumannii from intensive care units (ICUs) of Alzahra Hospital, Isfahan, Iran. MATERIALS AND METHODS: Seventy isolates of A. baumannii between March 2011 and June 2012 were studied. Susceptibility test was established by E-test method. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing was performed for detection of parC gene mutation. RESULTS: 77.1% of specimens were highly resistant. Mutation at position 80 in parC was observed in 93% of isolates. CONCLUSION: High proportion of A. baumannii isolates had a mutation in parC that can play an important role in increased incidence of these isolates. Medknow Publications & Media Pvt Ltd 2014-06 /pmc/articles/PMC4155714/ /pubmed/25197301 Text en Copyright: © Journal of Research in Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Vakili, Bahareh
Khorvash, Farzin
Fazeli, Hossein
Khaleghi, Moj
Detection of quinolone-resistance mutations of parC gene in clinical isolates of Acinetobacter baumannii in Iran
title Detection of quinolone-resistance mutations of parC gene in clinical isolates of Acinetobacter baumannii in Iran
title_full Detection of quinolone-resistance mutations of parC gene in clinical isolates of Acinetobacter baumannii in Iran
title_fullStr Detection of quinolone-resistance mutations of parC gene in clinical isolates of Acinetobacter baumannii in Iran
title_full_unstemmed Detection of quinolone-resistance mutations of parC gene in clinical isolates of Acinetobacter baumannii in Iran
title_short Detection of quinolone-resistance mutations of parC gene in clinical isolates of Acinetobacter baumannii in Iran
title_sort detection of quinolone-resistance mutations of parc gene in clinical isolates of acinetobacter baumannii in iran
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155714/
https://www.ncbi.nlm.nih.gov/pubmed/25197301
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