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Impurity of Stem Cell Graft by Murine Embryonic Fibroblasts – Implications for Cell-Based Therapy of the Central Nervous System

Stem cells have been demonstrated to possess a therapeutic potential in experimental models of various central nervous system disorders, including stroke. The types of implanted cells appear to play a crucial role. Previously, groups of the stem cell network NRW implemented a feeder-based cell line...

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Autores principales: Molcanyi, Marek, Mehrjardi, Narges Zare, Schäfer, Ute, Haj-Yasein, Nadia Nabil, Brockmann, Michael, Penner, Marina, Riess, Peter, Reinshagen, Clemens, Rieger, Bernhard, Hannes, Tobias, Hescheler, Jürgen, Bosche, Bert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155790/
https://www.ncbi.nlm.nih.gov/pubmed/25249934
http://dx.doi.org/10.3389/fncel.2014.00257
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author Molcanyi, Marek
Mehrjardi, Narges Zare
Schäfer, Ute
Haj-Yasein, Nadia Nabil
Brockmann, Michael
Penner, Marina
Riess, Peter
Reinshagen, Clemens
Rieger, Bernhard
Hannes, Tobias
Hescheler, Jürgen
Bosche, Bert
author_facet Molcanyi, Marek
Mehrjardi, Narges Zare
Schäfer, Ute
Haj-Yasein, Nadia Nabil
Brockmann, Michael
Penner, Marina
Riess, Peter
Reinshagen, Clemens
Rieger, Bernhard
Hannes, Tobias
Hescheler, Jürgen
Bosche, Bert
author_sort Molcanyi, Marek
collection PubMed
description Stem cells have been demonstrated to possess a therapeutic potential in experimental models of various central nervous system disorders, including stroke. The types of implanted cells appear to play a crucial role. Previously, groups of the stem cell network NRW implemented a feeder-based cell line within the scope of their projects, examining the implantation of stem cells after ischemic stroke and traumatic brain injury. Retrospective evaluation indicated the presence of spindle-shaped cells in several grafts implanted in injured animals, which indicated potential contamination by co-cultured feeder cells (murine embryonic fibroblasts – MEFs). Because feeder-based cell lines have been previously exposed to a justified criticism with regard to contamination by animal glycans, we aimed to evaluate the effects of stem cell/MEF co-transplantation. MEFs accounted for 5.3 ± 2.8% of all cells in the primary FACS-evaluated co-culture. Depending on the culture conditions and subsequent purification procedure, the MEF-fraction ranged from 0.9 to 9.9% of the cell suspensions in vitro. MEF survival and related formation of extracellular substances in vivo were observed after implantation into the uninjured rat brain. Impurity of the stem cell graft by MEFs interferes with translational strategies, which represents a threat to the potential recipient and may affect the graft microenvironment. The implications of these findings are critically discussed.
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spelling pubmed-41557902014-09-23 Impurity of Stem Cell Graft by Murine Embryonic Fibroblasts – Implications for Cell-Based Therapy of the Central Nervous System Molcanyi, Marek Mehrjardi, Narges Zare Schäfer, Ute Haj-Yasein, Nadia Nabil Brockmann, Michael Penner, Marina Riess, Peter Reinshagen, Clemens Rieger, Bernhard Hannes, Tobias Hescheler, Jürgen Bosche, Bert Front Cell Neurosci Neuroscience Stem cells have been demonstrated to possess a therapeutic potential in experimental models of various central nervous system disorders, including stroke. The types of implanted cells appear to play a crucial role. Previously, groups of the stem cell network NRW implemented a feeder-based cell line within the scope of their projects, examining the implantation of stem cells after ischemic stroke and traumatic brain injury. Retrospective evaluation indicated the presence of spindle-shaped cells in several grafts implanted in injured animals, which indicated potential contamination by co-cultured feeder cells (murine embryonic fibroblasts – MEFs). Because feeder-based cell lines have been previously exposed to a justified criticism with regard to contamination by animal glycans, we aimed to evaluate the effects of stem cell/MEF co-transplantation. MEFs accounted for 5.3 ± 2.8% of all cells in the primary FACS-evaluated co-culture. Depending on the culture conditions and subsequent purification procedure, the MEF-fraction ranged from 0.9 to 9.9% of the cell suspensions in vitro. MEF survival and related formation of extracellular substances in vivo were observed after implantation into the uninjured rat brain. Impurity of the stem cell graft by MEFs interferes with translational strategies, which represents a threat to the potential recipient and may affect the graft microenvironment. The implications of these findings are critically discussed. Frontiers Media S.A. 2014-09-05 /pmc/articles/PMC4155790/ /pubmed/25249934 http://dx.doi.org/10.3389/fncel.2014.00257 Text en Copyright © 2014 Molcanyi, Mehrjardi, Schäfer, Haj-Yasein, Brockmann, Penner, Riess, Reinshagen, Rieger, Hannes, Hescheler and Bosche. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Molcanyi, Marek
Mehrjardi, Narges Zare
Schäfer, Ute
Haj-Yasein, Nadia Nabil
Brockmann, Michael
Penner, Marina
Riess, Peter
Reinshagen, Clemens
Rieger, Bernhard
Hannes, Tobias
Hescheler, Jürgen
Bosche, Bert
Impurity of Stem Cell Graft by Murine Embryonic Fibroblasts – Implications for Cell-Based Therapy of the Central Nervous System
title Impurity of Stem Cell Graft by Murine Embryonic Fibroblasts – Implications for Cell-Based Therapy of the Central Nervous System
title_full Impurity of Stem Cell Graft by Murine Embryonic Fibroblasts – Implications for Cell-Based Therapy of the Central Nervous System
title_fullStr Impurity of Stem Cell Graft by Murine Embryonic Fibroblasts – Implications for Cell-Based Therapy of the Central Nervous System
title_full_unstemmed Impurity of Stem Cell Graft by Murine Embryonic Fibroblasts – Implications for Cell-Based Therapy of the Central Nervous System
title_short Impurity of Stem Cell Graft by Murine Embryonic Fibroblasts – Implications for Cell-Based Therapy of the Central Nervous System
title_sort impurity of stem cell graft by murine embryonic fibroblasts – implications for cell-based therapy of the central nervous system
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155790/
https://www.ncbi.nlm.nih.gov/pubmed/25249934
http://dx.doi.org/10.3389/fncel.2014.00257
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