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Knockout confirmation for Hurries: rapid genotype identification of Trypanosoma cruzi transfectants by polymerase chain reaction directly from liquid culture

Gene knockout is a widely used approach to evaluate loss-of-function phenotypes and it can be facilitated by the incorporation of a DNA cassette having a drug-selectable marker. Confirmation of the correct knockout cassette insertion is an important step in gene removal validation and has generally...

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Detalles Bibliográficos
Autores principales:	Alcantara, Monica Visnieski, Fragoso, Stenio Perdigão, Picchi/, Gisele Fernanda Assine
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Instituto Oswaldo Cruz, Ministério da Saúde 2014
Materias:	Technical Notes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155859/ https://www.ncbi.nlm.nih.gov/pubmed/24936912 http://dx.doi.org/10.1590/0074-0276140010

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