Cargando…

Knockout confirmation for Hurries: rapid genotype identification of Trypanosoma cruzi transfectants by polymerase chain reaction directly from liquid culture

Gene knockout is a widely used approach to evaluate loss-of-function phenotypes and it can be facilitated by the incorporation of a DNA cassette having a drug-selectable marker. Confirmation of the correct knockout cassette insertion is an important step in gene removal validation and has generally...

Descripción completa

Detalles Bibliográficos
Autores principales:	Alcantara, Monica Visnieski, Fragoso, Stenio Perdigão, Picchi/, Gisele Fernanda Assine
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Instituto Oswaldo Cruz, Ministério da Saúde 2014
Materias:	Technical Notes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4155859/ https://www.ncbi.nlm.nih.gov/pubmed/24936912 http://dx.doi.org/10.1590/0074-0276140010

Ejemplares similares

Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4-deficient Trypanosoma cruzi cells
por: Picchi-Constante, Gisele Fernanda Assine, et al.
Publicado: (2021)

Clathrin expression in Trypanosoma cruzi
por: Kalb, Ligia Cristina, et al.
Publicado: (2014)

In cellulo and in vivo assays for compound testing against Trypanosoma cruzi
por: Guerra-Slompo, Eloise Pavão, et al.
Publicado: (2023)

A novel nanoluciferase-based system to monitor Trypanosoma cruzi infection in mice by bioluminescence imaging
por: Silberstein, Erica, et al.
Publicado: (2018)

Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi
por: Picchi-Constante, Gisele Fernanda Assine, et al.
Publicado: (2022)

Knockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain
por: Moreira, Claudia Maria do Nascimento, et al.
Publicado: (2017)

Trypanosoma rangeli Transcriptome Project: Generation and analysis of expressed sequence tags
por: Snoeijer, Cristiane Quimelli, et al.
Publicado: (2004)

Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi
por: de Lima, Loyze P., et al.
Publicado: (2019)

The Influence of Recombinational Processes to Induce Dormancy in Trypanosoma cruzi
por: Resende, Bruno Carvalho, et al.
Publicado: (2020)

Expression and subcellular localization of kinetoplast-associated proteins in the different developmental stages of Trypanosoma cruzi
por: Cavalcanti, Danielle Pereira, et al.
Publicado: (2009)

Stage-Regulated GFP Expression in Trypanosoma cruzi: Applications from Host-Parasite Interactions to Drug Screening
por: Kessler, Rafael Luis, et al.
Publicado: (2013)

Knocking Down TcNTPDase-1 Gene Reduces in vitro Infectivity of Trypanosoma cruzi
por: Silva-Gomes, Natália Lins, et al.
Publicado: (2020)

Analysis of Proteasomal Proteolysis during the In Vitro Metacyclogenesis of Trypanosoma cruzi
por: Cardoso, Josiane, et al.
Publicado: (2011)

Hurry and Worry
Publicado: (1885)

In a Hurry
Publicado: (1876)

Hurrying to Death
Publicado: (1872)

Hurried Dinners
Publicado: (1893)

Replication Protein A Presents Canonical Functions and Is Also Involved in the Differentiation Capacity of Trypanosoma cruzi
por: Pavani, Raphael Souza, et al.
Publicado: (2016)

A high-throughput cloning system for reverse genetics in Trypanosoma cruzi
por: Batista, Michel, et al.
Publicado: (2010)

Evaluation of high efficiency gene knockout strategies for Trypanosoma cruzi
por: Xu, Dan, et al.
Publicado: (2009)

Hurry on down : a novel
por: Wain, John
Publicado: (1959)

Vaccines in a hurry
por: Søborg, Christian, et al.
Publicado: (2009)

pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation
por: Kugeratski, Fernanda G, et al.
Publicado: (2015)

International Enemy No. 1: Hurry
Publicado: (1948)

DNA Topoisomerase 3α Is Involved in Homologous Recombination Repair and Replication Stress Response in Trypanosoma cruzi
por: Costa-Silva, Héllida Marina, et al.
Publicado: (2021)

Astrophysics for young people in a hurry
por: Tyson, Neil Degrasse, et al.
Publicado: (2019)

Girls, Don’t Marry in a Hurry
Publicado: (1892)

Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent
por: Bodbin, Sara E., et al.
Publicado: (2020)

Overexpression of TcNTPDase-1 Gene Increases Infectivity in Mice Infected with Trypanosoma cruzi
por: da Silva-Gomes, Natália Lins, et al.
Publicado: (2022)

Geographical Distribution of Trypanosoma cruzi Genotypes in Venezuela
por: Carrasco, Hernán J., et al.
Publicado: (2012)

Collider may hurry age of 'new physics'
Publicado: (2008)

Inhibition of poly(ADP-ribose) Polymerase Interferes with Trypanosoma cruzi Infection and Proliferation of the Parasite
por: Vilchez Larrea, Salomé C., et al.
Publicado: (2012)

Retrospective distribution of Trypanosoma cruzi I genotypes in Colombia
por: León, Cielo M, et al.
Publicado: (2015)

Profiling the Trypanosoma cruzi Phosphoproteome
por: Marchini, Fabricio K., et al.
Publicado: (2011)

Endothelial Transmigration by Trypanosoma cruzi
por: Coates, Bria M., et al.
Publicado: (2013)

The N-myristoylome of Trypanosoma cruzi
por: Roberts, Adam J., et al.
Publicado: (2016)

Phenothiazinium Dyes Are Active against Trypanosoma cruzi In Vitro
por: Bulhões Portapilla, Gisele, et al.
Publicado: (2019)

Optimization and biological validation of an in vitro assay using the transfected Dm28c/pLacZ Trypanosoma cruzi strain
por: Gulin, Julián Ernesto Nicolás, et al.
Publicado: (2021)

Publish and Perish: The Dangers of Being Young and in a Hurry
por: Huntley, James S
Publicado: (2019)

Let's deconfine carefully for not having to reconfine in a hurry
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_oj0to1ck2ca786890rtuvt9f4l