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Quantitative histopathological assessment of ocular surface squamous neoplasia using digital image analysis

The aim of this retrospective pilot study was to evaluate the Aperio nuclear V9 algorithm as an image analysis tool to observe the histopathological changes of ocular surface squamous neoplasia (OSSN). A histopathological assessment, including the Ki-67 proliferative index (PI) of immunohistochemica...

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Autores principales: AŠOKLIS, RIMVYDAS, KADZIAUSKIENĖ, AISTĖ, PAULAVIČIENĖ, RASA, PETROŠKA, DONATAS, LAURINAVIČIUS, ARVYDAS
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4156187/
https://www.ncbi.nlm.nih.gov/pubmed/25202353
http://dx.doi.org/10.3892/ol.2014.2366
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author AŠOKLIS, RIMVYDAS
KADZIAUSKIENĖ, AISTĖ
PAULAVIČIENĖ, RASA
PETROŠKA, DONATAS
LAURINAVIČIUS, ARVYDAS
author_facet AŠOKLIS, RIMVYDAS
KADZIAUSKIENĖ, AISTĖ
PAULAVIČIENĖ, RASA
PETROŠKA, DONATAS
LAURINAVIČIUS, ARVYDAS
author_sort AŠOKLIS, RIMVYDAS
collection PubMed
description The aim of this retrospective pilot study was to evaluate the Aperio nuclear V9 algorithm as an image analysis tool to observe the histopathological changes of ocular surface squamous neoplasia (OSSN). A histopathological assessment, including the Ki-67 proliferative index (PI) of immunohistochemically-stained tumor conjunctiva (TC) and healthy conjunctiva (HC) tissues, was performed in six cases of OSSN. The Aperio V9 algorithm was applied to digital images of the tissue specimens to count the Ki-67 PI and to measure the nuclear area indices. This digital algorithm was validated using stereological and visual analysis methods. The visual scoring of Ki-67 PI ranged from 22 to 60% (mean, 38.5%), and from 5 to 20% (mean 9.5%) in TC and HC tissue, respectively. The computer-aided analysis, using the Aperio nuclear V9 algorithm, revealed that the Ki-67 PI ranged from 21.5 to 43.5% (mean, 33.6%), and from 1.9 to 21.0% (mean, 11.8%) in the TC and HC tissue, respectively. The stereological method demonstrated that the Ki-67 PI ranged from 30.1 to 51.5% (mean, 41.0%), and from 3.2 to 30.1% (mean, 15.1%) in the TC and HC tissues, respectively. The strongest association in the collinearity of regression analysis was observed between the Aperio nuclear V9 algorithm/stereological models in the TC tissue (r(2)=0.7; P=0.04) and the HC tissue (r(2)=0.7; P=0.03), and the visual/stereological models in the TC tissue (r(2)=0.7; P=0.04) and the visual/Aperio nuclear V9 algorithm models in the HC tissue (r(2)=0.7; P=0.04). A weak and statistically insignificant association was identified between the visual/Aperio nuclear V9 algorithm analysis in the TC tissue (r(2)=0.4; P=0.2) and the visual/stereological models in the HC tissue (r(2)=0.5; P=0.13). No significant difference was observed between the nuclear area of the TC (mean, 36.5 μm(2)) and HC (mean, 35.7 μm(2); P=0.88) tissues. It was concluded that the Aperio nuclear V9 algorithm is a useful tool for the reliable analysis of histopathological changes of OSSN. The results of this computer-aided algorithm correlate strongly with the stereological method when assessing the Ki-67 PI.
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spelling pubmed-41561872014-09-08 Quantitative histopathological assessment of ocular surface squamous neoplasia using digital image analysis AŠOKLIS, RIMVYDAS KADZIAUSKIENĖ, AISTĖ PAULAVIČIENĖ, RASA PETROŠKA, DONATAS LAURINAVIČIUS, ARVYDAS Oncol Lett Articles The aim of this retrospective pilot study was to evaluate the Aperio nuclear V9 algorithm as an image analysis tool to observe the histopathological changes of ocular surface squamous neoplasia (OSSN). A histopathological assessment, including the Ki-67 proliferative index (PI) of immunohistochemically-stained tumor conjunctiva (TC) and healthy conjunctiva (HC) tissues, was performed in six cases of OSSN. The Aperio V9 algorithm was applied to digital images of the tissue specimens to count the Ki-67 PI and to measure the nuclear area indices. This digital algorithm was validated using stereological and visual analysis methods. The visual scoring of Ki-67 PI ranged from 22 to 60% (mean, 38.5%), and from 5 to 20% (mean 9.5%) in TC and HC tissue, respectively. The computer-aided analysis, using the Aperio nuclear V9 algorithm, revealed that the Ki-67 PI ranged from 21.5 to 43.5% (mean, 33.6%), and from 1.9 to 21.0% (mean, 11.8%) in the TC and HC tissue, respectively. The stereological method demonstrated that the Ki-67 PI ranged from 30.1 to 51.5% (mean, 41.0%), and from 3.2 to 30.1% (mean, 15.1%) in the TC and HC tissues, respectively. The strongest association in the collinearity of regression analysis was observed between the Aperio nuclear V9 algorithm/stereological models in the TC tissue (r(2)=0.7; P=0.04) and the HC tissue (r(2)=0.7; P=0.03), and the visual/stereological models in the TC tissue (r(2)=0.7; P=0.04) and the visual/Aperio nuclear V9 algorithm models in the HC tissue (r(2)=0.7; P=0.04). A weak and statistically insignificant association was identified between the visual/Aperio nuclear V9 algorithm analysis in the TC tissue (r(2)=0.4; P=0.2) and the visual/stereological models in the HC tissue (r(2)=0.5; P=0.13). No significant difference was observed between the nuclear area of the TC (mean, 36.5 μm(2)) and HC (mean, 35.7 μm(2); P=0.88) tissues. It was concluded that the Aperio nuclear V9 algorithm is a useful tool for the reliable analysis of histopathological changes of OSSN. The results of this computer-aided algorithm correlate strongly with the stereological method when assessing the Ki-67 PI. D.A. Spandidos 2014-10 2014-07-22 /pmc/articles/PMC4156187/ /pubmed/25202353 http://dx.doi.org/10.3892/ol.2014.2366 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
AŠOKLIS, RIMVYDAS
KADZIAUSKIENĖ, AISTĖ
PAULAVIČIENĖ, RASA
PETROŠKA, DONATAS
LAURINAVIČIUS, ARVYDAS
Quantitative histopathological assessment of ocular surface squamous neoplasia using digital image analysis
title Quantitative histopathological assessment of ocular surface squamous neoplasia using digital image analysis
title_full Quantitative histopathological assessment of ocular surface squamous neoplasia using digital image analysis
title_fullStr Quantitative histopathological assessment of ocular surface squamous neoplasia using digital image analysis
title_full_unstemmed Quantitative histopathological assessment of ocular surface squamous neoplasia using digital image analysis
title_short Quantitative histopathological assessment of ocular surface squamous neoplasia using digital image analysis
title_sort quantitative histopathological assessment of ocular surface squamous neoplasia using digital image analysis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4156187/
https://www.ncbi.nlm.nih.gov/pubmed/25202353
http://dx.doi.org/10.3892/ol.2014.2366
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