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Ptaquiloside-induced cytotoxicity in Crandall feline kidney and HGC-27 cells

Ptaquiloside (PTA) is a potent genotoxic carcinogenic compound, which is found in bracken ferns and predominantly causes gastric tumors in humans, as well as bladder tumors and chronic enzootic hematuria in cattle. The underlying molecular mechanisms of PTA remain a topic for interdisciplinary inves...

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Autores principales: YURDAKOK, BEGUM, KISMALI, GORKEM, OZEN, DOGUKAN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4156249/
https://www.ncbi.nlm.nih.gov/pubmed/25202422
http://dx.doi.org/10.3892/ol.2014.2378
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author YURDAKOK, BEGUM
KISMALI, GORKEM
OZEN, DOGUKAN
author_facet YURDAKOK, BEGUM
KISMALI, GORKEM
OZEN, DOGUKAN
author_sort YURDAKOK, BEGUM
collection PubMed
description Ptaquiloside (PTA) is a potent genotoxic carcinogenic compound, which is found in bracken ferns and predominantly causes gastric tumors in humans, as well as bladder tumors and chronic enzootic hematuria in cattle. The underlying molecular mechanisms of PTA remain a topic for interdisciplinary investigation. The aim of the present study was to determine the possible cytotoxic effect of 24 h of PTA exposure in Crandall feline kidney (CrFK) and human gastric cells (the HGC-27 cell line) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and lactose dehydrogenase (LDH) analysis. The cytotoxic effects of PTA (0.0005–500 μg/ml) were found to increase in a dose-dependent manner, whereby the half maximal inhibitory concentration values were 11.17 and 11.86 μg/ml in the CrFK cells, and 2.03 and 2.56 μg/ml in the HGC-27 cells, by LDH and MTT assay, respectively. The results of the present study are consistent with those of previous studies associated with the cytotoxicity of PTA; however, cytotoxicity was identified to occur at significantly lower doses. This cytotoxic effect in vitro at particularly high doses may be linked to the initiation of carcinogenesis as a result of oxidative stress.
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spelling pubmed-41562492014-09-08 Ptaquiloside-induced cytotoxicity in Crandall feline kidney and HGC-27 cells YURDAKOK, BEGUM KISMALI, GORKEM OZEN, DOGUKAN Oncol Lett Articles Ptaquiloside (PTA) is a potent genotoxic carcinogenic compound, which is found in bracken ferns and predominantly causes gastric tumors in humans, as well as bladder tumors and chronic enzootic hematuria in cattle. The underlying molecular mechanisms of PTA remain a topic for interdisciplinary investigation. The aim of the present study was to determine the possible cytotoxic effect of 24 h of PTA exposure in Crandall feline kidney (CrFK) and human gastric cells (the HGC-27 cell line) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and lactose dehydrogenase (LDH) analysis. The cytotoxic effects of PTA (0.0005–500 μg/ml) were found to increase in a dose-dependent manner, whereby the half maximal inhibitory concentration values were 11.17 and 11.86 μg/ml in the CrFK cells, and 2.03 and 2.56 μg/ml in the HGC-27 cells, by LDH and MTT assay, respectively. The results of the present study are consistent with those of previous studies associated with the cytotoxicity of PTA; however, cytotoxicity was identified to occur at significantly lower doses. This cytotoxic effect in vitro at particularly high doses may be linked to the initiation of carcinogenesis as a result of oxidative stress. D.A. Spandidos 2014-10 2014-07-24 /pmc/articles/PMC4156249/ /pubmed/25202422 http://dx.doi.org/10.3892/ol.2014.2378 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
YURDAKOK, BEGUM
KISMALI, GORKEM
OZEN, DOGUKAN
Ptaquiloside-induced cytotoxicity in Crandall feline kidney and HGC-27 cells
title Ptaquiloside-induced cytotoxicity in Crandall feline kidney and HGC-27 cells
title_full Ptaquiloside-induced cytotoxicity in Crandall feline kidney and HGC-27 cells
title_fullStr Ptaquiloside-induced cytotoxicity in Crandall feline kidney and HGC-27 cells
title_full_unstemmed Ptaquiloside-induced cytotoxicity in Crandall feline kidney and HGC-27 cells
title_short Ptaquiloside-induced cytotoxicity in Crandall feline kidney and HGC-27 cells
title_sort ptaquiloside-induced cytotoxicity in crandall feline kidney and hgc-27 cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4156249/
https://www.ncbi.nlm.nih.gov/pubmed/25202422
http://dx.doi.org/10.3892/ol.2014.2378
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