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Hsa-microRNA-101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells

MicroRNAs (miRNAs) are 22- to 25-nucleotide non-coding RNA molecules that function as negative regulators of gene expression. In previous years, increasing evidence has arisen implicating the involvement of miRNAs in carcinogenesis. In previous studies, the role of miRNA-101 (miR-101) in tumors has...

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Autores principales: WANG, CHENGHAI, LU, SIJIA, JIANG, JIXIN, JIA, XIAOQIN, DONG, XIAOYUN, BU, PING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4156273/
https://www.ncbi.nlm.nih.gov/pubmed/25202416
http://dx.doi.org/10.3892/ol.2014.2361
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author WANG, CHENGHAI
LU, SIJIA
JIANG, JIXIN
JIA, XIAOQIN
DONG, XIAOYUN
BU, PING
author_facet WANG, CHENGHAI
LU, SIJIA
JIANG, JIXIN
JIA, XIAOQIN
DONG, XIAOYUN
BU, PING
author_sort WANG, CHENGHAI
collection PubMed
description MicroRNAs (miRNAs) are 22- to 25-nucleotide non-coding RNA molecules that function as negative regulators of gene expression. In previous years, increasing evidence has arisen implicating the involvement of miRNAs in carcinogenesis. In previous studies, the role of miRNA-101 (miR-101) in tumors has been identified as a tumor suppressor and, until now, the role of miR-101 and Rac1 in thyroid cancer has remained undefined. This study revealed that miR-101 is significantly downregulated in papillary thyroid carcinoma (PTC) tissue and thyroid cancer cell lines, and that the downregulated miR-101 is associated with lymph node metastasis. Infection with the miR-101 murine stem cell virus may markedly inhibit cell migration and invasion in TPC-1 and HTH83 thyroid cancer cell lines. Rac1 was demonstrated to be negatively regulated by miR-101 at the post-transcriptional level, via a specific target site within the 3′ untranslated region by dual-luciferase reporter assay. The expression of Rac1 was also observed to inversely correlate with miR-101 expression in PTC tissues; knockdown of Rac1 by shRNA inhibited thyroid cancer cell migration and invasion, resembling that of miR-101 overexpression. Thus, these findings suggested that miR-101 acts as a novel suppressor by targeting the Rac1 gene and inhibiting thyroid cancer cell migration and invasion.
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spelling pubmed-41562732014-09-08 Hsa-microRNA-101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells WANG, CHENGHAI LU, SIJIA JIANG, JIXIN JIA, XIAOQIN DONG, XIAOYUN BU, PING Oncol Lett Articles MicroRNAs (miRNAs) are 22- to 25-nucleotide non-coding RNA molecules that function as negative regulators of gene expression. In previous years, increasing evidence has arisen implicating the involvement of miRNAs in carcinogenesis. In previous studies, the role of miRNA-101 (miR-101) in tumors has been identified as a tumor suppressor and, until now, the role of miR-101 and Rac1 in thyroid cancer has remained undefined. This study revealed that miR-101 is significantly downregulated in papillary thyroid carcinoma (PTC) tissue and thyroid cancer cell lines, and that the downregulated miR-101 is associated with lymph node metastasis. Infection with the miR-101 murine stem cell virus may markedly inhibit cell migration and invasion in TPC-1 and HTH83 thyroid cancer cell lines. Rac1 was demonstrated to be negatively regulated by miR-101 at the post-transcriptional level, via a specific target site within the 3′ untranslated region by dual-luciferase reporter assay. The expression of Rac1 was also observed to inversely correlate with miR-101 expression in PTC tissues; knockdown of Rac1 by shRNA inhibited thyroid cancer cell migration and invasion, resembling that of miR-101 overexpression. Thus, these findings suggested that miR-101 acts as a novel suppressor by targeting the Rac1 gene and inhibiting thyroid cancer cell migration and invasion. D.A. Spandidos 2014-10 2014-07-18 /pmc/articles/PMC4156273/ /pubmed/25202416 http://dx.doi.org/10.3892/ol.2014.2361 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
WANG, CHENGHAI
LU, SIJIA
JIANG, JIXIN
JIA, XIAOQIN
DONG, XIAOYUN
BU, PING
Hsa-microRNA-101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells
title Hsa-microRNA-101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells
title_full Hsa-microRNA-101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells
title_fullStr Hsa-microRNA-101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells
title_full_unstemmed Hsa-microRNA-101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells
title_short Hsa-microRNA-101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells
title_sort hsa-microrna-101 suppresses migration and invasion by targeting rac1 in thyroid cancer cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4156273/
https://www.ncbi.nlm.nih.gov/pubmed/25202416
http://dx.doi.org/10.3892/ol.2014.2361
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