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Analysis of the effect of the active compound of green tea (EGCG) on the proliferation of peripheral blood mononuclear cells
BACKGROUND: Cancer immunotherapy requires proper manipulation of the immune system, lymphocytes in particular, in order to identify and destroy the cancer cells as non-self. In this study we investigated the effect of the flavonoid present in green tea, namely epigallocatechin-3-gallate (EGCG), on t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4156650/ https://www.ncbi.nlm.nih.gov/pubmed/25175005 http://dx.doi.org/10.1186/1472-6882-14-322 |
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author | Saleh, Farid Raghupathy, Raj Asfar, Sami Oteifa, Medhat Al-Saleh, Noha |
author_facet | Saleh, Farid Raghupathy, Raj Asfar, Sami Oteifa, Medhat Al-Saleh, Noha |
author_sort | Saleh, Farid |
collection | PubMed |
description | BACKGROUND: Cancer immunotherapy requires proper manipulation of the immune system, lymphocytes in particular, in order to identify and destroy the cancer cells as non-self. In this study we investigated the effect of the flavonoid present in green tea, namely epigallocatechin-3-gallate (EGCG), on the proliferation of, and IFN-γ production by, peripheral blood mononuclear cells (PBMC) from breast cancer patients stimulated with a mitogen, anti-CD3 and the common breast cancer peptides Her-2/neu, and p53. METHODS: Blood samples were collected from 25 patients with breast cancer at the Kuwait Cancer Control Centre (KCCC). The patients were newly diagnosed, and had not undergone any treatment or surgery at the time of sample collection. The control group consisted of 25 healthy women age-matched (±5 years) to the patients. PBMC were isolated from the patients and controls, and were cultured separately with the mitogen PHA, anti-CD3 antibodies, and Her-2/neu and p53 in the presence or absence of standardized doses of EGCG. The degree of proliferation and interferon-γ [IFN-γ) release were then analyzed. RESULTS: EGCG significantly suppressed the proliferation of PBMC in response to stimulation separately with (i) the mitogen, (ii) anti-CD3, and (iii) the cancer antigen peptides. IFN-γ production was also significantly suppressed by EGCG in vitro. CONCLUSIONS: EGCG appears to have an immunosuppressive effect on the proliferation of PBMC, indicating that EGCG is worth exploring for immunomodulatory effects in autoimmune diseases and tissue transplantation. |
format | Online Article Text |
id | pubmed-4156650 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41566502014-09-07 Analysis of the effect of the active compound of green tea (EGCG) on the proliferation of peripheral blood mononuclear cells Saleh, Farid Raghupathy, Raj Asfar, Sami Oteifa, Medhat Al-Saleh, Noha BMC Complement Altern Med Research Article BACKGROUND: Cancer immunotherapy requires proper manipulation of the immune system, lymphocytes in particular, in order to identify and destroy the cancer cells as non-self. In this study we investigated the effect of the flavonoid present in green tea, namely epigallocatechin-3-gallate (EGCG), on the proliferation of, and IFN-γ production by, peripheral blood mononuclear cells (PBMC) from breast cancer patients stimulated with a mitogen, anti-CD3 and the common breast cancer peptides Her-2/neu, and p53. METHODS: Blood samples were collected from 25 patients with breast cancer at the Kuwait Cancer Control Centre (KCCC). The patients were newly diagnosed, and had not undergone any treatment or surgery at the time of sample collection. The control group consisted of 25 healthy women age-matched (±5 years) to the patients. PBMC were isolated from the patients and controls, and were cultured separately with the mitogen PHA, anti-CD3 antibodies, and Her-2/neu and p53 in the presence or absence of standardized doses of EGCG. The degree of proliferation and interferon-γ [IFN-γ) release were then analyzed. RESULTS: EGCG significantly suppressed the proliferation of PBMC in response to stimulation separately with (i) the mitogen, (ii) anti-CD3, and (iii) the cancer antigen peptides. IFN-γ production was also significantly suppressed by EGCG in vitro. CONCLUSIONS: EGCG appears to have an immunosuppressive effect on the proliferation of PBMC, indicating that EGCG is worth exploring for immunomodulatory effects in autoimmune diseases and tissue transplantation. BioMed Central 2014-08-30 /pmc/articles/PMC4156650/ /pubmed/25175005 http://dx.doi.org/10.1186/1472-6882-14-322 Text en © Saleh et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Saleh, Farid Raghupathy, Raj Asfar, Sami Oteifa, Medhat Al-Saleh, Noha Analysis of the effect of the active compound of green tea (EGCG) on the proliferation of peripheral blood mononuclear cells |
title | Analysis of the effect of the active compound of green tea (EGCG) on the proliferation of peripheral blood mononuclear cells |
title_full | Analysis of the effect of the active compound of green tea (EGCG) on the proliferation of peripheral blood mononuclear cells |
title_fullStr | Analysis of the effect of the active compound of green tea (EGCG) on the proliferation of peripheral blood mononuclear cells |
title_full_unstemmed | Analysis of the effect of the active compound of green tea (EGCG) on the proliferation of peripheral blood mononuclear cells |
title_short | Analysis of the effect of the active compound of green tea (EGCG) on the proliferation of peripheral blood mononuclear cells |
title_sort | analysis of the effect of the active compound of green tea (egcg) on the proliferation of peripheral blood mononuclear cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4156650/ https://www.ncbi.nlm.nih.gov/pubmed/25175005 http://dx.doi.org/10.1186/1472-6882-14-322 |
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