Rapid Proton-Detected NMR Assignment for Proteins with Fast Magic Angle Spinning

[Image: see text] Using a set of six (1)H-detected triple-resonance NMR experiments, we establish a method for sequence-specific backbone resonance assignment of magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectra of 5–30 kDa proteins. The approach relies on perdeuteration, amide (2)...

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Autores principales: Barbet-Massin, Emeline, Pell, Andrew J., Retel, Joren S., Andreas, Loren B., Jaudzems, Kristaps, Franks, W. Trent, Nieuwkoop, Andrew J., Hiller, Matthias, Higman, Victoria, Guerry, Paul, Bertarello, Andrea, Knight, Michael J., Felletti, Michele, Le Marchand, Tanguy, Kotelovica, Svetlana, Akopjana, Inara, Tars, Kaspars, Stoppini, Monica, Bellotti, Vittorio, Bolognesi, Martino, Ricagno, Stefano, Chou, James J., Griffin, Robert G., Oschkinat, Hartmut, Lesage, Anne, Emsley, Lyndon, Herrmann, Torsten, Pintacuda, Guido
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4156866/
https://www.ncbi.nlm.nih.gov/pubmed/25102442
http://dx.doi.org/10.1021/ja507382j

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