Total Phenolic Contents and Antioxidant Activities of Different Extracts and Fractions from the Aerial Parts of Artemisia biennis Willd

Total phenolic contents (TPC) of five different extracts (petroleum ether, dichloromethane, ethyl acetate, ethanol and ethanol-water) of Artemisia biennis Willd were measured in this work. The antioxidant activity was investigated by three different methods: β-carotene bleaching (BCB) test, 2,2-diph...

Descripción completa

Detalles Bibliográficos
Autores principales: Hatami, Tayyebe, Emami, Sayyed Ahmad, Miraghaee, Sayyed Shahram, Mojarrab, Mahdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2014
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157030/
https://www.ncbi.nlm.nih.gov/pubmed/25237350
_version_ 1782333816946294784
author Hatami, Tayyebe
Emami, Sayyed Ahmad
Miraghaee, Sayyed Shahram
Mojarrab, Mahdi
author_facet Hatami, Tayyebe
Emami, Sayyed Ahmad
Miraghaee, Sayyed Shahram
Mojarrab, Mahdi
author_sort Hatami, Tayyebe
collection PubMed
description Total phenolic contents (TPC) of five different extracts (petroleum ether, dichloromethane, ethyl acetate, ethanol and ethanol-water) of Artemisia biennis Willd were measured in this work. The antioxidant activity was investigated by three different methods: β-carotene bleaching (BCB) test, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and ferrous ion chelating (FIC) assay. Among all the extracts analyzed, the hydroethanolic extract exhibited a significantly higher phenolic content and antioxidant activity than other samples. Vacuum liquid chromatography of this extract yielded seven fractions (A to G) which were subjected to all aforementioned experiments. The highest total phenolic content and free radical scavenging activites were present in the same sample (Fraction D) but the only statistically significant correlation between TPC and EC(50) values was observed for BCB.
format Online
Article
Text
id pubmed-4157030
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Shaheed Beheshti University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-41570302014-09-18 Total Phenolic Contents and Antioxidant Activities of Different Extracts and Fractions from the Aerial Parts of Artemisia biennis Willd Hatami, Tayyebe Emami, Sayyed Ahmad Miraghaee, Sayyed Shahram Mojarrab, Mahdi Iran J Pharm Res Original Article Total phenolic contents (TPC) of five different extracts (petroleum ether, dichloromethane, ethyl acetate, ethanol and ethanol-water) of Artemisia biennis Willd were measured in this work. The antioxidant activity was investigated by three different methods: β-carotene bleaching (BCB) test, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and ferrous ion chelating (FIC) assay. Among all the extracts analyzed, the hydroethanolic extract exhibited a significantly higher phenolic content and antioxidant activity than other samples. Vacuum liquid chromatography of this extract yielded seven fractions (A to G) which were subjected to all aforementioned experiments. The highest total phenolic content and free radical scavenging activites were present in the same sample (Fraction D) but the only statistically significant correlation between TPC and EC(50) values was observed for BCB. Shaheed Beheshti University of Medical Sciences 2014 /pmc/articles/PMC4157030/ /pubmed/25237350 Text en © 2014 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Hatami, Tayyebe
Emami, Sayyed Ahmad
Miraghaee, Sayyed Shahram
Mojarrab, Mahdi
Total Phenolic Contents and Antioxidant Activities of Different Extracts and Fractions from the Aerial Parts of Artemisia biennis Willd
title Total Phenolic Contents and Antioxidant Activities of Different Extracts and Fractions from the Aerial Parts of Artemisia biennis Willd
title_full Total Phenolic Contents and Antioxidant Activities of Different Extracts and Fractions from the Aerial Parts of Artemisia biennis Willd
title_fullStr Total Phenolic Contents and Antioxidant Activities of Different Extracts and Fractions from the Aerial Parts of Artemisia biennis Willd
title_full_unstemmed Total Phenolic Contents and Antioxidant Activities of Different Extracts and Fractions from the Aerial Parts of Artemisia biennis Willd
title_short Total Phenolic Contents and Antioxidant Activities of Different Extracts and Fractions from the Aerial Parts of Artemisia biennis Willd
title_sort total phenolic contents and antioxidant activities of different extracts and fractions from the aerial parts of artemisia biennis willd
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157030/
https://www.ncbi.nlm.nih.gov/pubmed/25237350
work_keys_str_mv AT hatamitayyebe totalphenoliccontentsandantioxidantactivitiesofdifferentextractsandfractionsfromtheaerialpartsofartemisiabienniswilld
AT emamisayyedahmad totalphenoliccontentsandantioxidantactivitiesofdifferentextractsandfractionsfromtheaerialpartsofartemisiabienniswilld
AT miraghaeesayyedshahram totalphenoliccontentsandantioxidantactivitiesofdifferentextractsandfractionsfromtheaerialpartsofartemisiabienniswilld
AT mojarrabmahdi totalphenoliccontentsandantioxidantactivitiesofdifferentextractsandfractionsfromtheaerialpartsofartemisiabienniswilld

Ejemplares similares