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Synergistic Activity of Econazole-Nitrate and Chelerythrine against Clinical Isolates of Candida albicans

The aim of this investigation was to assess the in-vitro interaction of two antifungal agents, econazole-nitrate and chelerythrine, against ten fluconazole-resistant clinical isolates and one ATCC type strain 10231 of Candida albicans. The checkerboard microdilution method was performed according to...

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Autores principales: Chen, Zhibao, Li, Xinran, Wu, Xiuping, Wang, Wei, Wang, Wendong, Xin, Mingxun, Shen, Fengge, Liu, Lihui, Liang, Junchao, Li, Lei, Yu, Lu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157032/
https://www.ncbi.nlm.nih.gov/pubmed/25237352
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author Chen, Zhibao
Li, Xinran
Wu, Xiuping
Wang, Wei
Wang, Wendong
Xin, Mingxun
Shen, Fengge
Liu, Lihui
Liang, Junchao
Li, Lei
Yu, Lu
author_facet Chen, Zhibao
Li, Xinran
Wu, Xiuping
Wang, Wei
Wang, Wendong
Xin, Mingxun
Shen, Fengge
Liu, Lihui
Liang, Junchao
Li, Lei
Yu, Lu
author_sort Chen, Zhibao
collection PubMed
description The aim of this investigation was to assess the in-vitro interaction of two antifungal agents, econazole-nitrate and chelerythrine, against ten fluconazole-resistant clinical isolates and one ATCC type strain 10231 of Candida albicans. The checkerboard microdilution method was performed according to the recommendations of the National Committee for Clinical Laboratory Standards, and the results were determined by visual examination. The interaction intensity was tested in all isolates using the fractional inhibitory concentration index (FICI). These experiments showed synergism between econazole-nitrate and chelerythrine in antifungal activity against C. albicans, and no antagonistic activity was observed in any of the strains tested. Moreover, time-kill curves were performed with selected strains to confirm the positive interactions. The similarity between the results of the FICI values and the time-kill curves revealed that chelerythrine greatly enhances the antifungal effects of econazole-nitrate against isolates of C. albicans. This synergistic effect may markedly reduce the dose of econazole-nitrate required to treat candidiasis, thereby decreasing the econazole-nitrate toxic side effects. This novel synergism might provide a potential combination treatment against fungal infections.
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spelling pubmed-41570322014-09-18 Synergistic Activity of Econazole-Nitrate and Chelerythrine against Clinical Isolates of Candida albicans Chen, Zhibao Li, Xinran Wu, Xiuping Wang, Wei Wang, Wendong Xin, Mingxun Shen, Fengge Liu, Lihui Liang, Junchao Li, Lei Yu, Lu Iran J Pharm Res Original Article The aim of this investigation was to assess the in-vitro interaction of two antifungal agents, econazole-nitrate and chelerythrine, against ten fluconazole-resistant clinical isolates and one ATCC type strain 10231 of Candida albicans. The checkerboard microdilution method was performed according to the recommendations of the National Committee for Clinical Laboratory Standards, and the results were determined by visual examination. The interaction intensity was tested in all isolates using the fractional inhibitory concentration index (FICI). These experiments showed synergism between econazole-nitrate and chelerythrine in antifungal activity against C. albicans, and no antagonistic activity was observed in any of the strains tested. Moreover, time-kill curves were performed with selected strains to confirm the positive interactions. The similarity between the results of the FICI values and the time-kill curves revealed that chelerythrine greatly enhances the antifungal effects of econazole-nitrate against isolates of C. albicans. This synergistic effect may markedly reduce the dose of econazole-nitrate required to treat candidiasis, thereby decreasing the econazole-nitrate toxic side effects. This novel synergism might provide a potential combination treatment against fungal infections. Shaheed Beheshti University of Medical Sciences 2014 /pmc/articles/PMC4157032/ /pubmed/25237352 Text en © 2014 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Chen, Zhibao
Li, Xinran
Wu, Xiuping
Wang, Wei
Wang, Wendong
Xin, Mingxun
Shen, Fengge
Liu, Lihui
Liang, Junchao
Li, Lei
Yu, Lu
Synergistic Activity of Econazole-Nitrate and Chelerythrine against Clinical Isolates of Candida albicans
title Synergistic Activity of Econazole-Nitrate and Chelerythrine against Clinical Isolates of Candida albicans
title_full Synergistic Activity of Econazole-Nitrate and Chelerythrine against Clinical Isolates of Candida albicans
title_fullStr Synergistic Activity of Econazole-Nitrate and Chelerythrine against Clinical Isolates of Candida albicans
title_full_unstemmed Synergistic Activity of Econazole-Nitrate and Chelerythrine against Clinical Isolates of Candida albicans
title_short Synergistic Activity of Econazole-Nitrate and Chelerythrine against Clinical Isolates of Candida albicans
title_sort synergistic activity of econazole-nitrate and chelerythrine against clinical isolates of candida albicans
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157032/
https://www.ncbi.nlm.nih.gov/pubmed/25237352
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