Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells

For the first time we show the effects of deuterium oxide on cell growth and vesicle transport in rat basophilic leukemia (RBL-2H3) cells. RBL-2H3 cells cultured with 15 moles/L deuterium showed decreased cell growth which was attributed to cells not doubling their DNA content. Experimental observat...

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Autores principales: Kalkur, Roshni S., Ballast, Andrew C., Triplett, Ashley R., Spendier, Kathrin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2014
Materias:
Biophysics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157235/
https://www.ncbi.nlm.nih.gov/pubmed/25237603
http://dx.doi.org/10.7717/peerj.553
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author Kalkur, Roshni S.
Ballast, Andrew C.
Triplett, Ashley R.
Spendier, Kathrin
author_facet Kalkur, Roshni S.
Ballast, Andrew C.
Triplett, Ashley R.
Spendier, Kathrin
author_sort Kalkur, Roshni S.
collection PubMed
description For the first time we show the effects of deuterium oxide on cell growth and vesicle transport in rat basophilic leukemia (RBL-2H3) cells. RBL-2H3 cells cultured with 15 moles/L deuterium showed decreased cell growth which was attributed to cells not doubling their DNA content. Experimental observations also showed an increase in vesicle speed for cells cultured in deuterium oxide. This increase in vesicle speed was not observed in deuterium oxide cultures treated with a microtubule-destabilizing drug, suggesting that deuterium oxide affects microtubule-dependent vesicle transport.
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spelling pubmed-41572352014-09-18 Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells Kalkur, Roshni S. Ballast, Andrew C. Triplett, Ashley R. Spendier, Kathrin PeerJ Biophysics For the first time we show the effects of deuterium oxide on cell growth and vesicle transport in rat basophilic leukemia (RBL-2H3) cells. RBL-2H3 cells cultured with 15 moles/L deuterium showed decreased cell growth which was attributed to cells not doubling their DNA content. Experimental observations also showed an increase in vesicle speed for cells cultured in deuterium oxide. This increase in vesicle speed was not observed in deuterium oxide cultures treated with a microtubule-destabilizing drug, suggesting that deuterium oxide affects microtubule-dependent vesicle transport. PeerJ Inc. 2014-09-02 /pmc/articles/PMC4157235/ /pubmed/25237603 http://dx.doi.org/10.7717/peerj.553 Text en © 2014 Kalkur et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biophysics
Kalkur, Roshni S.
Ballast, Andrew C.
Triplett, Ashley R.
Spendier, Kathrin
Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells
title Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells
title_full Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells
title_fullStr Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells
title_full_unstemmed Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells
title_short Effects of deuterium oxide on cell growth and vesicle speed in RBL-2H3 cells
title_sort effects of deuterium oxide on cell growth and vesicle speed in rbl-2h3 cells
topic Biophysics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157235/
https://www.ncbi.nlm.nih.gov/pubmed/25237603
http://dx.doi.org/10.7717/peerj.553
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