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Evaluation of a semiquantitative SNAP test for measurement of bile acids in dogs
Background. Serum bile acids (SBA) are used as a routine screening tool of liver function in dogs. Serum samples are usually shipped to a referral laboratory for quantitative analysis with an enzymatic chemistry analyzer. The canine SNAP Bile Acids Test (SNAP-BAT) provides an immediate, semi-quantit...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157295/ https://www.ncbi.nlm.nih.gov/pubmed/25210659 http://dx.doi.org/10.7717/peerj.539 |
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author | Seibert, Rachel L. Tobias, Karen M. Reed, Ann Snyder, Karl R. |
author_facet | Seibert, Rachel L. Tobias, Karen M. Reed, Ann Snyder, Karl R. |
author_sort | Seibert, Rachel L. |
collection | PubMed |
description | Background. Serum bile acids (SBA) are used as a routine screening tool of liver function in dogs. Serum samples are usually shipped to a referral laboratory for quantitative analysis with an enzymatic chemistry analyzer. The canine SNAP Bile Acids Test (SNAP-BAT) provides an immediate, semi-quantitative measurement of bile acid concentrations in-house. With the SNAP-BAT, bile acids concentrations of 5–30 µmol/L are quantified, and results outside of that range are classified as <5 or >30 µmol/L. Agreement of the SNAP-BAT with the enzymatic method has not been extensively investigated. Objectives. The purposes of this prospective clinical study were to assess the precision of the SNAP-BAT and determine agreement of SNAP-BAT with results from an in-house chemistry analyzer. Methods. After verifying intra-assay precision of the SNAP-BAT, a prospective analysis was performed using blood samples collected from 56 dogs suspected to have liver disease. Each sample was analyzed with an enzymatic, in-house chemistry analyzer and the SNAP-BAT. Agreement between the two methods was statistically assessed using the κ index of agreement. Results. Intra-assay variability was minimal. The κ index for agreement between the SNAP-BAT and routine chemistry analyzer was between 0.752 and 0.819, indicating substantial to near perfect agreement. Conclusions. The SNAP-BAT is a highly accurate, semi-quantitative test that yields immediate results, and has very little intra-assay variability, particularly for results >30 µmol/L. |
format | Online Article Text |
id | pubmed-4157295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-41572952014-09-10 Evaluation of a semiquantitative SNAP test for measurement of bile acids in dogs Seibert, Rachel L. Tobias, Karen M. Reed, Ann Snyder, Karl R. PeerJ Veterinary Medicine Background. Serum bile acids (SBA) are used as a routine screening tool of liver function in dogs. Serum samples are usually shipped to a referral laboratory for quantitative analysis with an enzymatic chemistry analyzer. The canine SNAP Bile Acids Test (SNAP-BAT) provides an immediate, semi-quantitative measurement of bile acid concentrations in-house. With the SNAP-BAT, bile acids concentrations of 5–30 µmol/L are quantified, and results outside of that range are classified as <5 or >30 µmol/L. Agreement of the SNAP-BAT with the enzymatic method has not been extensively investigated. Objectives. The purposes of this prospective clinical study were to assess the precision of the SNAP-BAT and determine agreement of SNAP-BAT with results from an in-house chemistry analyzer. Methods. After verifying intra-assay precision of the SNAP-BAT, a prospective analysis was performed using blood samples collected from 56 dogs suspected to have liver disease. Each sample was analyzed with an enzymatic, in-house chemistry analyzer and the SNAP-BAT. Agreement between the two methods was statistically assessed using the κ index of agreement. Results. Intra-assay variability was minimal. The κ index for agreement between the SNAP-BAT and routine chemistry analyzer was between 0.752 and 0.819, indicating substantial to near perfect agreement. Conclusions. The SNAP-BAT is a highly accurate, semi-quantitative test that yields immediate results, and has very little intra-assay variability, particularly for results >30 µmol/L. PeerJ Inc. 2014-08-26 /pmc/articles/PMC4157295/ /pubmed/25210659 http://dx.doi.org/10.7717/peerj.539 Text en © 2014 Seibert et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Veterinary Medicine Seibert, Rachel L. Tobias, Karen M. Reed, Ann Snyder, Karl R. Evaluation of a semiquantitative SNAP test for measurement of bile acids in dogs |
title | Evaluation of a semiquantitative SNAP test for measurement of bile acids in dogs |
title_full | Evaluation of a semiquantitative SNAP test for measurement of bile acids in dogs |
title_fullStr | Evaluation of a semiquantitative SNAP test for measurement of bile acids in dogs |
title_full_unstemmed | Evaluation of a semiquantitative SNAP test for measurement of bile acids in dogs |
title_short | Evaluation of a semiquantitative SNAP test for measurement of bile acids in dogs |
title_sort | evaluation of a semiquantitative snap test for measurement of bile acids in dogs |
topic | Veterinary Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157295/ https://www.ncbi.nlm.nih.gov/pubmed/25210659 http://dx.doi.org/10.7717/peerj.539 |
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