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Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin

Many studies have compared the genetic and epigenetic profiles of human induced pluripotent stem cells (hiPSCs) to human embryonic stem cells (hESCs) and yet the picture remains unclear. To address this, we derived a population of neural precursor cells (NPCs) from the H1 (WA01) hESC line and genera...

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Autores principales: Mallon, Barbara S., Hamilton, Rebecca S., Kozhich, Olga A., Johnson, Kory R., Fann, Yang C., Rao, Mahendra S., Robey, Pamela G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157340/
https://www.ncbi.nlm.nih.gov/pubmed/24374290
http://dx.doi.org/10.1016/j.scr.2013.11.010
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author Mallon, Barbara S.
Hamilton, Rebecca S.
Kozhich, Olga A.
Johnson, Kory R.
Fann, Yang C.
Rao, Mahendra S.
Robey, Pamela G.
author_facet Mallon, Barbara S.
Hamilton, Rebecca S.
Kozhich, Olga A.
Johnson, Kory R.
Fann, Yang C.
Rao, Mahendra S.
Robey, Pamela G.
author_sort Mallon, Barbara S.
collection PubMed
description Many studies have compared the genetic and epigenetic profiles of human induced pluripotent stem cells (hiPSCs) to human embryonic stem cells (hESCs) and yet the picture remains unclear. To address this, we derived a population of neural precursor cells (NPCs) from the H1 (WA01) hESC line and generated isogenic iPSC lines by reprogramming. The gene expression and methylation profile of three lines were compared to the parental line and intermediate NPC population. We found no gene probe with expression that differed significantly between hESC and iPSC samples under undifferentiated or differentiated conditions. Analysis of the global methylation pattern also showed no significant difference between the two PSC populations. Both undifferentiated populations were distinctly different from the intermediate NPC population in both gene expression and methylation profiles. One point to note is that H1 is a male line and so extrapolation to female lines should be cautioned. However, these data confirm our previous findings that there are no significant differences between hESCs and hiPSCs at the gene expression or methylation level.
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spelling pubmed-41573402015-03-01 Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin Mallon, Barbara S. Hamilton, Rebecca S. Kozhich, Olga A. Johnson, Kory R. Fann, Yang C. Rao, Mahendra S. Robey, Pamela G. Stem Cell Res Article Many studies have compared the genetic and epigenetic profiles of human induced pluripotent stem cells (hiPSCs) to human embryonic stem cells (hESCs) and yet the picture remains unclear. To address this, we derived a population of neural precursor cells (NPCs) from the H1 (WA01) hESC line and generated isogenic iPSC lines by reprogramming. The gene expression and methylation profile of three lines were compared to the parental line and intermediate NPC population. We found no gene probe with expression that differed significantly between hESC and iPSC samples under undifferentiated or differentiated conditions. Analysis of the global methylation pattern also showed no significant difference between the two PSC populations. Both undifferentiated populations were distinctly different from the intermediate NPC population in both gene expression and methylation profiles. One point to note is that H1 is a male line and so extrapolation to female lines should be cautioned. However, these data confirm our previous findings that there are no significant differences between hESCs and hiPSCs at the gene expression or methylation level. 2013-12-04 2014-03 /pmc/articles/PMC4157340/ /pubmed/24374290 http://dx.doi.org/10.1016/j.scr.2013.11.010 Text en © 2013 The Authors. Published by Elsevier B.V. All rights reserved. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Mallon, Barbara S.
Hamilton, Rebecca S.
Kozhich, Olga A.
Johnson, Kory R.
Fann, Yang C.
Rao, Mahendra S.
Robey, Pamela G.
Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin
title Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin
title_full Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin
title_fullStr Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin
title_full_unstemmed Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin
title_short Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin
title_sort comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157340/
https://www.ncbi.nlm.nih.gov/pubmed/24374290
http://dx.doi.org/10.1016/j.scr.2013.11.010
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