Cargando…

Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells

Periodontal repair is a complex process in which regeneration of alveolar bone is a vital component. The aim of this study was to develop a biodegradable scaffold with good biocompatibility and osteoinductive ability. Two types of composite fibrous scaffolds were produced by electrospinning, ie, typ...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Xiaonan, Miao, Leiying, Yao, Yingfang, Wu, Wenlei, Liu, Yu, Chen, Xiaofeng, Sun, Weibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157625/
https://www.ncbi.nlm.nih.gov/pubmed/25206304
http://dx.doi.org/10.2147/IJN.S65272
_version_ 1782333909715910656
author Wu, Xiaonan
Miao, Leiying
Yao, Yingfang
Wu, Wenlei
Liu, Yu
Chen, Xiaofeng
Sun, Weibin
author_facet Wu, Xiaonan
Miao, Leiying
Yao, Yingfang
Wu, Wenlei
Liu, Yu
Chen, Xiaofeng
Sun, Weibin
author_sort Wu, Xiaonan
collection PubMed
description Periodontal repair is a complex process in which regeneration of alveolar bone is a vital component. The aim of this study was to develop a biodegradable scaffold with good biocompatibility and osteoinductive ability. Two types of composite fibrous scaffolds were produced by electrospinning, ie, type I collagen/poly(ε-caprolactone) (COL/PCL) and type I collagen/poly(ε-caprolactone)/nanoscale hydroxyapatite (COL/PCL/nHA) with an average fiber diameter of about 377 nm. After a simulated body fluid (SBF) immersion test, the COL/PCL/nHA-SBF scaffold developed a rough surface because of the calcium phosphate deposited on the fibers, suggesting that the presence of nHA promoted the mineralization potential of the scaffold. Energy dispersive X-ray spectroscopy clearly showed the calcium and phosphorus content in the COL/PCL/nHA and COL/PCL/nHA-SBF scaffolds, confirming the findings of nHA and calcium phosphate precipitation on scanning electron micrographs. Water contact analysis revealed that nHA could improve the hydrophilic nature of the COL/PCL/nHA-SBF scaffold. The morphology of periodontal ligament cells cultured on COL/PCL-SBF and COL/PCL/nHA-SBF was evaluated by scanning electron microscopy. The results showed that cells adhered to either type of scaffold and were slightly spindle-shaped in the beginning, then extended gradually with stretched filopodia, indicating an ability to fill the fiber pores. A Cell Counting Kit-8 assay showed that both scaffolds supported cell proliferation. However, real-time quantitative polymerase chain reaction analysis showed that expression of the bone-related markers, alkaline phosphatase and osteocalcin, was upregulated only on the COL/PCL/nHA-SBF scaffold, indicating that this scaffold had the ability to induce osteogenic differentiation of periodontal ligament cells. In this study, COL/PCL/nHA-SBF produced by electrospinning followed by biomimetic mineralization had combined electrospun fibers with nHA in it. This scaffold has good biocompatibility and osteoinductive ability as a result of the characteristics of nHA, so could be innovatively applied to periodontal tissue engineering as a potential scaffold.
format Online
Article
Text
id pubmed-4157625
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-41576252014-09-09 Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells Wu, Xiaonan Miao, Leiying Yao, Yingfang Wu, Wenlei Liu, Yu Chen, Xiaofeng Sun, Weibin Int J Nanomedicine Original Research Periodontal repair is a complex process in which regeneration of alveolar bone is a vital component. The aim of this study was to develop a biodegradable scaffold with good biocompatibility and osteoinductive ability. Two types of composite fibrous scaffolds were produced by electrospinning, ie, type I collagen/poly(ε-caprolactone) (COL/PCL) and type I collagen/poly(ε-caprolactone)/nanoscale hydroxyapatite (COL/PCL/nHA) with an average fiber diameter of about 377 nm. After a simulated body fluid (SBF) immersion test, the COL/PCL/nHA-SBF scaffold developed a rough surface because of the calcium phosphate deposited on the fibers, suggesting that the presence of nHA promoted the mineralization potential of the scaffold. Energy dispersive X-ray spectroscopy clearly showed the calcium and phosphorus content in the COL/PCL/nHA and COL/PCL/nHA-SBF scaffolds, confirming the findings of nHA and calcium phosphate precipitation on scanning electron micrographs. Water contact analysis revealed that nHA could improve the hydrophilic nature of the COL/PCL/nHA-SBF scaffold. The morphology of periodontal ligament cells cultured on COL/PCL-SBF and COL/PCL/nHA-SBF was evaluated by scanning electron microscopy. The results showed that cells adhered to either type of scaffold and were slightly spindle-shaped in the beginning, then extended gradually with stretched filopodia, indicating an ability to fill the fiber pores. A Cell Counting Kit-8 assay showed that both scaffolds supported cell proliferation. However, real-time quantitative polymerase chain reaction analysis showed that expression of the bone-related markers, alkaline phosphatase and osteocalcin, was upregulated only on the COL/PCL/nHA-SBF scaffold, indicating that this scaffold had the ability to induce osteogenic differentiation of periodontal ligament cells. In this study, COL/PCL/nHA-SBF produced by electrospinning followed by biomimetic mineralization had combined electrospun fibers with nHA in it. This scaffold has good biocompatibility and osteoinductive ability as a result of the characteristics of nHA, so could be innovatively applied to periodontal tissue engineering as a potential scaffold. Dove Medical Press 2014-08-27 /pmc/articles/PMC4157625/ /pubmed/25206304 http://dx.doi.org/10.2147/IJN.S65272 Text en © 2014 Wu et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Wu, Xiaonan
Miao, Leiying
Yao, Yingfang
Wu, Wenlei
Liu, Yu
Chen, Xiaofeng
Sun, Weibin
Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells
title Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells
title_full Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells
title_fullStr Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells
title_full_unstemmed Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells
title_short Electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells
title_sort electrospun fibrous scaffolds combined with nanoscale hydroxyapatite induce osteogenic differentiation of human periodontal ligament cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4157625/
https://www.ncbi.nlm.nih.gov/pubmed/25206304
http://dx.doi.org/10.2147/IJN.S65272
work_keys_str_mv AT wuxiaonan electrospunfibrousscaffoldscombinedwithnanoscalehydroxyapatiteinduceosteogenicdifferentiationofhumanperiodontalligamentcells
AT miaoleiying electrospunfibrousscaffoldscombinedwithnanoscalehydroxyapatiteinduceosteogenicdifferentiationofhumanperiodontalligamentcells
AT yaoyingfang electrospunfibrousscaffoldscombinedwithnanoscalehydroxyapatiteinduceosteogenicdifferentiationofhumanperiodontalligamentcells
AT wuwenlei electrospunfibrousscaffoldscombinedwithnanoscalehydroxyapatiteinduceosteogenicdifferentiationofhumanperiodontalligamentcells
AT liuyu electrospunfibrousscaffoldscombinedwithnanoscalehydroxyapatiteinduceosteogenicdifferentiationofhumanperiodontalligamentcells
AT chenxiaofeng electrospunfibrousscaffoldscombinedwithnanoscalehydroxyapatiteinduceosteogenicdifferentiationofhumanperiodontalligamentcells
AT sunweibin electrospunfibrousscaffoldscombinedwithnanoscalehydroxyapatiteinduceosteogenicdifferentiationofhumanperiodontalligamentcells