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Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1

BACKGROUND: Brassinosteroid hormones regulate many aspects of plant growth and development. The membrane receptor BRI1 is a central player in the brassinosteroid signaling cascade. Semi-dwarf ‘uzu’ barley carries a mutation in a conserved domain of the kinase tail of BRI1 and this mutant allele is r...

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Autores principales: Ali, Shahin S, Gunupuru, Lokanadha R, Kumar, G B Sunil, Khan, Mojibur, Scofield, Steve, Nicholson, Paul, Doohan, Fiona M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4158134/
https://www.ncbi.nlm.nih.gov/pubmed/25135116
http://dx.doi.org/10.1186/s12870-014-0227-1
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author Ali, Shahin S
Gunupuru, Lokanadha R
Kumar, G B Sunil
Khan, Mojibur
Scofield, Steve
Nicholson, Paul
Doohan, Fiona M
author_facet Ali, Shahin S
Gunupuru, Lokanadha R
Kumar, G B Sunil
Khan, Mojibur
Scofield, Steve
Nicholson, Paul
Doohan, Fiona M
author_sort Ali, Shahin S
collection PubMed
description BACKGROUND: Brassinosteroid hormones regulate many aspects of plant growth and development. The membrane receptor BRI1 is a central player in the brassinosteroid signaling cascade. Semi-dwarf ‘uzu’ barley carries a mutation in a conserved domain of the kinase tail of BRI1 and this mutant allele is recognised for its positive contribution to both yield and lodging resistance. RESULTS: Here we show that uzu barley exhibits enhanced resistance to a range of pathogens. It was due to a combination of preformed, inducible and constitutive defence responses, as determined by a combination of transcriptomic and biochemical studies. Gene expression studies were used to determine that the uzu derivatives are attenuated in downstream brassinosteroid signaling. The reduction of BRI1 RNA levels via virus-induced gene silencing compromised uzu disease resistance. CONCLUSIONS: The pathogen resistance of uzu derivatives may be due to pleiotropic effects of BRI1 or the cascade effects of their repressed BR signaling. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-014-0227-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-41581342014-09-10 Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1 Ali, Shahin S Gunupuru, Lokanadha R Kumar, G B Sunil Khan, Mojibur Scofield, Steve Nicholson, Paul Doohan, Fiona M BMC Plant Biol Research Article BACKGROUND: Brassinosteroid hormones regulate many aspects of plant growth and development. The membrane receptor BRI1 is a central player in the brassinosteroid signaling cascade. Semi-dwarf ‘uzu’ barley carries a mutation in a conserved domain of the kinase tail of BRI1 and this mutant allele is recognised for its positive contribution to both yield and lodging resistance. RESULTS: Here we show that uzu barley exhibits enhanced resistance to a range of pathogens. It was due to a combination of preformed, inducible and constitutive defence responses, as determined by a combination of transcriptomic and biochemical studies. Gene expression studies were used to determine that the uzu derivatives are attenuated in downstream brassinosteroid signaling. The reduction of BRI1 RNA levels via virus-induced gene silencing compromised uzu disease resistance. CONCLUSIONS: The pathogen resistance of uzu derivatives may be due to pleiotropic effects of BRI1 or the cascade effects of their repressed BR signaling. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-014-0227-1) contains supplementary material, which is available to authorized users. BioMed Central 2014-08-20 /pmc/articles/PMC4158134/ /pubmed/25135116 http://dx.doi.org/10.1186/s12870-014-0227-1 Text en © Ali et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ali, Shahin S
Gunupuru, Lokanadha R
Kumar, G B Sunil
Khan, Mojibur
Scofield, Steve
Nicholson, Paul
Doohan, Fiona M
Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1
title Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1
title_full Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1
title_fullStr Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1
title_full_unstemmed Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1
title_short Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1
title_sort plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor bri1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4158134/
https://www.ncbi.nlm.nih.gov/pubmed/25135116
http://dx.doi.org/10.1186/s12870-014-0227-1
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