Dew inspired breathing-based detection of genetic point mutation visualized by naked eye

A novel label-free method based on breathing-induced vapor condensation was developed for detection of genetic point mutation. The dew-inspired detection was realized by integration of target-induced DNA ligation with rolling circle amplification (RCA). The vapor condensation induced by breathing tr...

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Autores principales: Xie, Liping, Wang, Tongzhou, Huang, Tianqi, Hou, Wei, Huang, Guoliang, Du, Yanan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4158330/
https://www.ncbi.nlm.nih.gov/pubmed/25199907
http://dx.doi.org/10.1038/srep06300
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author Xie, Liping
Wang, Tongzhou
Huang, Tianqi
Hou, Wei
Huang, Guoliang
Du, Yanan
author_facet Xie, Liping
Wang, Tongzhou
Huang, Tianqi
Hou, Wei
Huang, Guoliang
Du, Yanan
author_sort Xie, Liping
collection PubMed
description A novel label-free method based on breathing-induced vapor condensation was developed for detection of genetic point mutation. The dew-inspired detection was realized by integration of target-induced DNA ligation with rolling circle amplification (RCA). The vapor condensation induced by breathing transduced the RCA-amplified variances in DNA contents into visible contrast. The image could be recorded by a cell phone for further or even remote analysis. This green assay offers a naked-eye-reading method potentially applied for point-of-care liver cancer diagnosis in resource-limited regions.
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spelling pubmed-41583302014-09-10 Dew inspired breathing-based detection of genetic point mutation visualized by naked eye Xie, Liping Wang, Tongzhou Huang, Tianqi Hou, Wei Huang, Guoliang Du, Yanan Sci Rep Article A novel label-free method based on breathing-induced vapor condensation was developed for detection of genetic point mutation. The dew-inspired detection was realized by integration of target-induced DNA ligation with rolling circle amplification (RCA). The vapor condensation induced by breathing transduced the RCA-amplified variances in DNA contents into visible contrast. The image could be recorded by a cell phone for further or even remote analysis. This green assay offers a naked-eye-reading method potentially applied for point-of-care liver cancer diagnosis in resource-limited regions. Nature Publishing Group 2014-09-09 /pmc/articles/PMC4158330/ /pubmed/25199907 http://dx.doi.org/10.1038/srep06300 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Article
Xie, Liping
Wang, Tongzhou
Huang, Tianqi
Hou, Wei
Huang, Guoliang
Du, Yanan
Dew inspired breathing-based detection of genetic point mutation visualized by naked eye
title Dew inspired breathing-based detection of genetic point mutation visualized by naked eye
title_full Dew inspired breathing-based detection of genetic point mutation visualized by naked eye
title_fullStr Dew inspired breathing-based detection of genetic point mutation visualized by naked eye
title_full_unstemmed Dew inspired breathing-based detection of genetic point mutation visualized by naked eye
title_short Dew inspired breathing-based detection of genetic point mutation visualized by naked eye
title_sort dew inspired breathing-based detection of genetic point mutation visualized by naked eye
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4158330/
https://www.ncbi.nlm.nih.gov/pubmed/25199907
http://dx.doi.org/10.1038/srep06300
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